Mesenchymal Stem Cells Derived from CD133‐Positive Cells in Mobilized Peripheral Blood and Cord Blood: Proliferation, Oct4 Expression, and Plasticity

Tondreau, Tatiana; Meuleman, Nathalie; Delforge, Alain; Dejeneffe, Marielle; Leroy, Rita; Massy, Martine; Mortier, Christine; Bron, Dominique; Lagneaux, Laurence

doi:10.1634/stemcells.2004-0330

Cited by 405 publications

(287 citation statements)

References 42 publications

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“…However, similar to MSCs, these cells were able to differentiate into adipocytes and osteocytes, suggesting that they are a higher cell lineage than cartilagecommitted progenitor cells, which are predetermined to form chondrocytes of elastic cartilage. In line with these expectations, CSPCs expressed CD44 and CD90, similar to MSCs, whereas they did not express the MSC markers CD133, CD140a, CD146, or CD271 (24,33,34). Thus, our isolated stem/progenitor cells are closely related to MSCs, yet are a distinct cartilage stem/ progenitor cell population.…”

Section: Discussionsupporting

confidence: 82%

Reconstruction of human elastic cartilage by a CD44 ⁺ CD90 ⁺ stem cell in the ear perichondrium

Kobayashi

Takebe²,

Inui³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Despite the great demands for treating craniofacial injuries or abnormalities, effective treatments are currently lacking. One promising approach involves human elastic cartilage reconstruction using autologous stem/progenitor populations. Nevertheless, definitive evidence of the presence of stem cells in human auricular cartilage remains to be established. Here, we demonstrate that human auricular perichondrium, which can be obtained via a minimally invasive approach, harbors a unique cell population, termed as cartilage stem/progenitor cells (CSPCs). The clonogenic progeny of a single CD44 + CD90 + CSPC displays a number of features characteristic of stem cells. Highly chondrogenic CSPCs were shown to reconstruct large (>2 cm) elastic cartilage after extended expansion and differentiation. CSPC-derived cartilage was encapsulated by a perichondrium layer, which contains a CD44 + CD90 + self-renewing stem/progenitor population and was maintained without calcification or tumor formation even after 10 mo. This is a unique report demonstrating the presence of stem cells in auricular cartilage. Utilization of CSPCs will provide a promising reconstructive material for treating craniofacial defects with successful long-term tissue restoration.

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Section: Discussionsupporting

confidence: 82%

Reconstruction of human elastic cartilage by a CD44 ⁺ CD90 ⁺ stem cell in the ear perichondrium

Kobayashi

Takebe²,

Inui³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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“…However, several differences between human and murine systems cannot be ignored; these differences include the expression patterns of surface antigens in MPCs, for instance (7,44,51,53). Moreover, human cells are difficult to transform in vitro, and the transformed cells of mice seem to produce a more aggressive tumor than those of hu-* Corresponding author.…”

mentioning

confidence: 99%

Inducible Expression of Chimeric EWS/ETS Proteins Confers Ewing's Family Tumor-Like Phenotypes to Human Mesenchymal Progenitor Cells

Miyagawa

Okita

Nakaijima

et al. 2008

Molecular and Cellular Biology

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Ewing's family tumor (EFT) is a rare pediatric tumor of unclear origin that occurs in bone and soft tissue. Specific chromosomal translocations found in EFT cause EWS to fuse to a subset of ets transcription factor genes (ETS), generating chimeric EWS/ETS proteins. These proteins are believed to play a crucial role in the onset and progression of EFT. However, the mechanisms responsible for the EWS/ETS-mediated onset remain unclear. Here we report the establishment of a tetracycline-controlled EWS/ETS-inducible system in human bone marrow-derived mesenchymal progenitor cells (MPCs). Ectopic expression of both EWS/FLI1 and EWS/ERG proteins resulted in a dramatic change of morphology, i.e., from a mesenchymal spindle shape to a small round-to-polygonal cell, one of the characteristics of EFT. EWS/ETS also induced immunophenotypic changes in MPCs, including the disappearance of the mesenchyme-positive markers CD10 and CD13 and the up-regulation of the EFT-positive markers CD54, CD99, CD117, and CD271. Furthermore, a prominent shift from the gene expression profile of MPCs to that of EFT was observed in the presence of EWS/ETS. Together with the observation that EWS/ETS enhances the ability of cells to invade Matrigel, these results suggest that EWS/ETS proteins contribute to alterations of cellular features and confer an EFT-like phenotype to human MPCs.Ewing's family tumor (EFT) is a rare childhood cancer arising mainly in bone and soft tissue. Since EFT has a poor prognosis, it is important to elucidate the underlying pathogenic mechanisms for establishing a more effective therapeutic strategy. EFT is characterized by the presence of chimeric genes composed of EWS and ets transcription factor genes (ETS) formed by specific chromosomal translocations, i.e., EWS/FLI1, t(11;22)(q24;q12); EWS/ERG, t(21;22)(q12;q12); EWS/ETV1, t(7;22)(p22;q12); EWS/E1AF, t(17;22)(q12;q12); and EWS/FEV, t(2;22)(q33;q12) (26). The products of these chimeric genes behave as aberrant transcriptional regulators and are believed to play a crucial role in the onset and progression of EFT (3,36). Indeed, recent studies have revealed that the induction of EWS/FLI1 proteins can trigger transformation in certain cell types, including NIH 3T3 cells (36), C2C12 myoblasts (12), and murine primary bone marrow-derived mesenchymal progenitor cells (MPCs) (6, 45, 52). However, studies have also indicated that overexpression of EWS/ FLI1 provokes apoptosis and growth arrest in mouse normal embryonic fibroblasts and primary human fibroblasts (10, 31), hence hampering understanding of the precise role of EWS/ ETS proteins in the development of EFT. The function of EWS/ETS proteins would be greatly influenced by cell type, and thus the cells that can originate EFTs might be more susceptible to the tumorigenic effects of EWS/ETS.Although the cell origin of EFT is still unknown, the expression of neuronal markers in spite of the occurrence in bone and soft tissues has kept open the debate as to a potential mesenchymal or neuroectodermal origin. As described ...

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“…There have been many suggestions that MNCs might also be used for tissue regeneration because it was found that MSCs could be cultured and isolated from the MNC fractions of BM, CB, and mPB [7][8][9]. The effects of MSCs and MNCs have been compared in various experimental settings ( [19][20][21].…”

Section: Mscs Vs Mncs For Cell Therapymentioning

confidence: 99%

Regenerative Potential of Intravenous Infusion with Mononuclear Cells in Cord Blood and G-CSF-Mobilized Peripheral Blood

Lee¹

2014

J Stem Cell Res Ther

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Mesenchymal Stem Cells Derived from CD133‐Positive Cells in Mobilized Peripheral Blood and Cord Blood: Proliferation, Oct4 Expression, and Plasticity

Abstract: In this study, we used a common procedure to assess the potential of mobilized peripheral blood (MPB) and umbilical cord blood (

Cited by 405 publications

References 42 publications

Reconstruction of human elastic cartilage by a CD44 ⁺ CD90 ⁺ stem cell in the ear perichondrium

Reconstruction of human elastic cartilage by a CD44 ⁺ CD90 ⁺ stem cell in the ear perichondrium

Inducible Expression of Chimeric EWS/ETS Proteins Confers Ewing's Family Tumor-Like Phenotypes to Human Mesenchymal Progenitor Cells

Regenerative Potential of Intravenous Infusion with Mononuclear Cells in Cord Blood and G-CSF-Mobilized Peripheral Blood

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Mesenchymal Stem Cells Derived from CD133‐Positive Cells in Mobilized Peripheral Blood and Cord Blood: Proliferation, Oct4 Expression, and Plasticity

Abstract: In this study, we used a common procedure to assess the potential of mobilized peripheral blood (MPB) and umbilical cord blood (

Cited by 405 publications

References 42 publications

Reconstruction of human elastic cartilage by a CD44 + CD90 + stem cell in the ear perichondrium

Reconstruction of human elastic cartilage by a CD44 + CD90 + stem cell in the ear perichondrium

Inducible Expression of Chimeric EWS/ETS Proteins Confers Ewing's Family Tumor-Like Phenotypes to Human Mesenchymal Progenitor Cells

Regenerative Potential of Intravenous Infusion with Mononuclear Cells in Cord Blood and G-CSF-Mobilized Peripheral Blood

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Reconstruction of human elastic cartilage by a CD44 ⁺ CD90 ⁺ stem cell in the ear perichondrium

Reconstruction of human elastic cartilage by a CD44 ⁺ CD90 ⁺ stem cell in the ear perichondrium