Mesenchymal stem cells increase antioxidant capacity in intestinal ischemia/reperfusion damage

İnan, Mustafa; Bakar, Elvan; Cerkezkayabekir, Aysegul; Sanal, Filiz; Uluçam, Enis; Subaşı, Cansu; Karaöz, Erdal

doi:10.1016/j.jpedsurg.2016.12.024

Cited by 38 publications

(30 citation statements)

References 30 publications

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“…Plasma cytokine profiles might reflect dynamics of organ injury and recruited repair mechanisms after partial hepatectomy. Others have already demonstrated the antioxidant capacity of MSC treatment in intestinal ischemia reperfusion damage, 53 myocardial infarction, 54 and diabetic rats. 55 Malondialdehyde as a marker for antioxidant capacity was significantly lower after MSC treatment, and the authors suggest a support of the antioxidant defense system because of stem cell treatment.…”

Section: Discussionmentioning

confidence: 99%

Human Stem Cells Promote Liver Regeneration After Partial Hepatectomy in BALB/C Nude Mice

Wabitsch

Benzing

Krenzien

et al. 2019

Journal of Surgical Research

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Background: Mesenchymal stem cells (MSCs) have been suggested to augment liver regeneration after surgically and pharmacologically induced liver failure. To further investigate this we processed human bone marrow-derived MSC according to good manufacturing practice (GMP) and tested those cells for their modulatory capacities of metabolic alterations and liver regeneration after partial hepatectomy in BALB/c nude mice. Methods: Human MSCs were obtained by bone marrow aspiration of healthy donors as in a previously described GMP process. Transgenic GFP-MSCs were administered i.p. 24 h after 70% hepatectomy in BALB/c nude mice, whereas control mice received phosphate-buffered saline. Mice were sacrificed 2, 3, and 5 d after partial hepatectomy. Blood and organs were harvested and metabolic alterations as well as liver regeneration subsequently assessed by liver function tests, multianalyte profiling immunoassays, histology, and immunostaining. Results: Hepatocyte and sinusoidal endothelial cell proliferation were significantly increased after partial hepatectomy in mice receiving MSC compared to control mice (Hepatocyte postoperative day 3, P < 0.01; endothelial cell postoperative day 5, P < 0.05). Hepatocyte fat accumulation correlated inversely with hepatocyte proliferation (r 2 ¼ 0.4064, P < 0.01) 2 d after partial hepatectomy, with mice receiving MSC being protected from severe fat accumulation. No GFP-positive cells could be detected in the samples. Serum levels of IL-6, HGF, and IL-10 were significantly decreased at day 3 in mice receiving MSC when compared to control mice (P < 0.05). Relative body weight loss was significantly attenuated after partial hepatectomy in mice receiving MSC (2 d and 3 d, both P < 0.001) with a trend toward a faster relative restoration of liver weight, when compared to control mice. Conclusions: Human bone marrow-derived MSC attenuate metabolic alterations and improve liver regeneration after partial hepatectomy in BALB/c nude mice. Obtained

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Section: Discussionmentioning

confidence: 99%

Human Stem Cells Promote Liver Regeneration After Partial Hepatectomy in BALB/C Nude Mice

Wabitsch

Benzing

Krenzien

et al. 2019

Journal of Surgical Research

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“…MSC treatment led to a slight increase in TPO activity, which is different from neither control nor DM groups. Mesenchymal stem cells have been shown to regulate other peroxidases, such as myeloperoxidase and glutathione peroxidase [37]. This slight change in TPO activity, however, was not great enough to normalize thyroid hormone synthesis, which remained similar in DM and DM + MSC.…”

Section: Discussionmentioning

confidence: 97%

Influence of Stem Cell Therapy on Thyroid Function and Reactive Oxygen Species Production in Diabetic Rats

Silva

Freitas

Monnerat³

et al. 2018

Horm Metab Res

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Cell therapy with mesenchymal stem cells (MSC) has been proposed for the treatment of diabetes mellitus (DM). It is known that the prevalence of thyroid disease is higher among diabetic patients than in general population. Therefore, our aim was to investigate the effect of the treatment with MSC on thyroid function and ROS generation in an experimental model of type 1 DM. Adult male Wistar rats were divided into the following groups: control, DM (80 mg/kg BW streptozotocin, iv.) and DM+MSC. MSC treatment occurred 4 weeks after DM induction and the animals were euthanized 4 weeks after MSC administration. We also evaluated the effect of co-culture with MSC or extracellular vesicles (EV) obtained from these cells on the rat thyroid cell line PCCL3 exposed to high glucose. Thyroid HO generation was increased in DM, which was reversed by MSC treatment. These changes paralled a significant DuOx1 mRNA increase. The incubation of PCCL3 with high glucose increased extracellular HO generation, which was reversed by both the co-culture with MSC and EV. Even though MSC treatment normalized thyroid ROS generation, serum thyroid hormone (TH) concentration remained low, along with increased serum TSH concentrations. Thyroperoxidase (TPO) activity, was reduced in DM, and MSC treatment did not normalize TPO. Therefore, we conclude that the treatment with MSC was able to reverse the increased thyroid HO generation in diabetic animals and in PCCL3 cells exposed to high glucose, an effect probably mediated by EV produced by these cells, acting in a paracrine fashion.

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“…When the efficacy of the stem cell applications in ischemic reperfusion injury is examined, it is observed that Inan et al have shown that the levels of interleukin 1β (IL-1β), transforming growth factor-1β, tumor necrosis factor-α and IL-6, which are pro-inflammatory cytokines, decreased in the stem cell treatment groups after the application of MSCs in ischemic reperfusion injury. In the same study, it was shown that MSCs could promote antioxidant enzymes, which can decrease oxidative stress by reducing MDA levels (12). Yin et al reported that administration of MSCs in a spinal cord ischemia-reperfusion injury showed an anti-apoptotic activity on the medulla spinalis and that it might protect the spinal cord against ischemic-reperfusion injury (40).…”

Section: █ Discussionmentioning

confidence: 98%

Effect of systemic application of bone marrow-derived mesenchymal stem cells on healing of peripheral nerve injury in an experimental sciatic nerve injury model

Kızılay

Aktaş²,

Çetin³

et al. 2017

Turkish Neurosurgery

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Mesenchymal stem cells increase antioxidant capacity in intestinal ischemia/reperfusion damage

Cited by 38 publications

References 30 publications

Human Stem Cells Promote Liver Regeneration After Partial Hepatectomy in BALB/C Nude Mice

Human Stem Cells Promote Liver Regeneration After Partial Hepatectomy in BALB/C Nude Mice

Influence of Stem Cell Therapy on Thyroid Function and Reactive Oxygen Species Production in Diabetic Rats

Effect of systemic application of bone marrow-derived mesenchymal stem cells on healing of peripheral nerve injury in an experimental sciatic nerve injury model

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