Mesenchymal stem cells modified with angiopoietin-1 improve remodeling in a rat model of acute myocardial infarction

Sun, Lijie; Cui, Ming; Wang, Zuoyan; Feng, Xing; Mao, Jiandong; Chen, Ping; Ma, Ke; Chen, Fengrong; Zhou, Chunyan

doi:10.1016/j.bbrc.2007.04.010

Cited by 69 publications

(35 citation statements)

References 15 publications

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“…Twenty sections per heart were captured as digital image, and five fields per section were calculated and the collagen-delegated infarction percentage was analyzed by a blinded investigator. The calculation formula used for the infracted size was: % infract size = infarct areas/total left ventricle area Â 100% [16].…”

Section: Histologymentioning

confidence: 99%

Effects of combined mesenchymal stem cells and heme oxygenase-1 therapy on cardiac performance☆

Zeng

Chen

Zhu

et al. 2008

European Journal of Cardio-Thoracic Surgery

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Section: Histologymentioning

confidence: 99%

Effects of combined mesenchymal stem cells and heme oxygenase-1 therapy on cardiac performance☆

Zeng

Chen

Zhu

et al. 2008

European Journal of Cardio-Thoracic Surgery

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“…Ang-1 gene has also been reported to improve the blood flow in ischemic heart or limb diseases [7][8][9]. Therefore, the combination of Ang-1 and MSCs has become an alternative method to enhance angiogenesis [10,11]. However, when performing the combination of gene and MSCs therapy, a vector that has high transductive efficiency to MSCs is needed.…”

Section: Introductionmentioning

confidence: 97%

Transplantation of sendai viral angiopoietin-1-modified mesenchymal stem cells for ischemic limb disease

et al. 2010

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Sendai viral vector (SeV) is emerging as a promising vector for gene therapy. However, little information is available regarding the combination of SeV-mediated gene and mesenchymal stem cell (MSC) therapy in dealing with ischemic diseases. In this study, we infected SeV to the MSCs in vitro; and injected MSCs modified with SeV harboring human angiopoietin-1 gene (SeVhAng-1) into the ischemic limb of rats in vivo. We found SeV had high transductive efficiency to the MSCs. Both MSCs and SeVhAng-1-modified MSCs improved the blood flow recovery and increased the capillary density of the ischemic limb, compared with the control. However, in contrast to MSCs, SeVhAng-1-modified MSCs had a better improvement of blood flow recovery in the ischemic limb. We further found the ischemic limb injected with SeVhAng-1-modified MSCs had strong expression of p-Akt, which improved survival of MSCs injected into the ischemic limb. This indicated SeVhAng-1 modification enhanced angiogenetic effect of MSCs by both angiogenesis and cell protection. We conclude that SeVhAng-1-modified MSCs may serve as a more effective tool in dealing with ischemic limb disease.

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“…BMSCs are strongly adherent, highly proliferative and readily available; therefore, they have become the ideal seed cells to use in tissue engineering [Kemp et al, 2005;Janssens et al, 2006;Lunde et al, 2006;Sun et al, 2007]. Target tissues used for tissue engineering repair (for example, bone and cartilage) are weight-bearing organs, and mechanical stimulation plays an important role in the morphology, development and function of cells from these tissues.…”

Section: Introductionmentioning

confidence: 99%

Estrogen and Its Receptor Enhance Mechanobiological Effects in Compressed Bone Mesenchymal Stem Cells

Zhang

Chen²,

Wang³

et al. 2011

Cells Tissues Organs

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Mechanical stimulation and estrogen have been proven to be two important factors in promoting mesenchymal stem cell activity, which is closely associated with bone formation, mass maintenance and remodeling. However, the superposition effects of mechanical stimulation and estrogen on stem cells remain unknown. It is also unclear if the estrogen receptor (ER) plays only a key role in estrogen signaling or if it is also involved in the mechanotransduction of stem cells. To investigate the role of estrogen and its receptors in the mechanobiological effects in bone mesenchymal stem cells (BMSCs), isolated mesenchymal stem cells from bone marrow were exposed to mechanical pressure under additional estrogen treatment or ER blockade. Cell proliferation was examined using an MTT assay and alkaline phosphatase (ALP) activity was determined by a modified enzyme kinetic method. Alignment of the cytoskeleton was observed by Coomassie brilliant blue staining and F-actin fluorescent staining. Cellular ultrastructure was observed under transmission electron microscope. Expression of ERα was investigated using Western blot analysis. Results indicated that mechanical pressure promoted cell proliferation, ALP activity, ERα expression and F-actin stress fiber formation. Overall, this effect was enhanced by the addition of estrogen and inhibited by ER blockade. We concluded that pressure stimulated proliferation and differentiation capability via F-actin transduction in BMSCs. The effects were enhanced by the addition of estrogen, and the ER plays an important role in regulating mechanobiological effects and the mechanotransduction processes of BMSCs.

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Mesenchymal stem cells modified with angiopoietin-1 improve remodeling in a rat model of acute myocardial infarction

Cited by 69 publications

References 15 publications

Effects of combined mesenchymal stem cells and heme oxygenase-1 therapy on cardiac performance☆

Effects of combined mesenchymal stem cells and heme oxygenase-1 therapy on cardiac performance☆

Transplantation of sendai viral angiopoietin-1-modified mesenchymal stem cells for ischemic limb disease

Estrogen and Its Receptor Enhance Mechanobiological Effects in Compressed Bone Mesenchymal Stem Cells

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