Mesenchymal stem cells: paradoxes of passaging

Javazon, Elisabeth H.; Beggs, Kirstin J.; Flake, Alan W.

doi:10.1016/j.exphem.2004.02.004

Cited by 464 publications

(359 citation statements)

References 185 publications

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“…In contrast to the increasing applications of MSCs in adult bone marrow, DA-MSC MIGRATION BY EMBRYONIC CIRCULATION 67 the understanding of embryonic MSC is lagging far behind, especially the ontogeny, developmental origin, and migration (Javazon et al, 2004). In this study, we examined the multi-potentiality and phenotypic properties of MSC populations derived from mouse E11.5 DA and E12.5-E13.5 embryonic circulation.…”

Section: Discussionmentioning

confidence: 99%

Migration of dorsal aorta mesenchymal stem cells induced by mouse embryonic circulation

Yan

Lan

Wang³

et al. 2010

Developmental Dynamics

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Mesenchymal stem cells (MSCs) represent powerful tools for regenerative medicine for their differentiation and migration capacity. However, ontogeny and migration of MSCs in mammalian mid-gestation conceptus is poorly understood. We identified canonical MSCs in the mouse embryonic day (E) 11.5 dorsal aorta (DA). They possessed homogenous immunophenotype (CD45 2 CD31 2 Flk-1 2 CD44 1 CD29 1 ), expressed perivascular markers (a-SMA 1 NG2 1 PDGFRb 1 PDGFRa 1 ), and had tri-lineage differentiation potential (osteoblasts, adipocytes, and chondrocytes). Of interest, MSCs were also detected in E12.5-E13.5 embryonic circulation, 24 hr later than in DA, suggesting migration like hematopoietic stem cells. Functionally, E12.5 embryonic blood could trigger efficient migration of DA-MSCs through platelet-derived growth factor (PDGF) receptor-, transforming growth factor-beta receptor-, but not basic fibroblast growth factor receptor-mediated signaling. Moreover, downstream JNK and AKT signaling pathway played important roles in embryonic blood-or PDGF-mediated migration of DA-derived MSCs. Taken together, these results revealed that clonal MSCs developed in the mouse DA. More importantly, the embryonic circulation, in addition to its conventional transporting roles, could modulate migration of MSC during early embryogenesis. Developmental Dynamics 240:65-74,

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Section: Discussionmentioning

confidence: 99%

Migration of dorsal aorta mesenchymal stem cells induced by mouse embryonic circulation

Yan

Lan

Wang³

et al. 2010

Developmental Dynamics

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“…Mesenchymal stromal cells (MSCs), originally isolated from adult bone marrow (BM), are multipotent cells that provide stromal micro-environmental support for hematopoietic stem cells (HSCs); as well as give rise to mesodermal tissues such as bone, fat and cartilage [1][2][3][4]. Over the last several years MSCs have been derived from a variety of other tissues of adult [5][6][7] or fetal origin [8,9].…”

Section: Introductionmentioning

confidence: 99%

Derivation and immunological characterization of mesenchymal stromal cells from human embryonic stem cells

Trivedi

Hematti

2008

Experimental Hematology

178

159

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Objective-We have previously shown the simultaneous generation of CD73+ mesenchymal stromal cells (MSCs) along with CD34+ hematopoietic cells from human embryonic stem cells (ESCs) when they are co-cultured with OP9 murine stromal cells. We investigated whether MSCs can be derived from human ESCs without co-culturing with OP9 cells, and if such cells exhibit immunological properties similar to MSCs derived from adult human bone marrow (BM).Materials and Methods-Our starting populations were undifferentiated ESCs cultured on matrigel-coated plates without feeder cells. The differentiated fibroblast-looking cells were tested for expression of MSC markers and their potential for multi-lineage differentiation. We investigated surface expression of HLA molecules on these MSCs before and after treatment with interferon gamma (IFN-γ). We also tested the proliferative response of T-lymphocytes towards MSCs and the effects of MSCs in mixed lymphocyte reaction (MLR) assays. Conclusions-MSCs can be derived from human ESCs without feeder cells. These human ESCderived MSCs have cell surface markers, differentiation potentials, and immunological properties in vitro that are similar to adult BM-derived MSCs. Results-We

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“…On another side a full characterization of MSCs biology in vitro is still not available and several doubts remain on the phenotypes they can develop and on the conditions required [Javazon et al, 2004]. At this regard recent results from Bayes-Genis et al [2005] report that human MSCs spontaneously express markers of cardiac phenotype in vitro.…”

mentioning

confidence: 99%

“…Thus, many issues remain to be clarified on the processes following MSCs culture [Javazon et al, 2004] and transplantation in the myocardium. In particular, the cellular mechanism involved in the interaction between MSCs and recipient's adult cardiomyocytes has not yet been fully described.…”

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confidence: 99%

Limited plasticity of mesenchymal stem cells cocultured with adult cardiomyocytes

Gallo¹,

Ramella²,

Alloatti³

et al. 2006

J of Cellular Biochemistry

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In order to assess, in a controlled in vitro model, the differentiation potential of adult bone marrow derived stem cells we have developed a coculture procedure using adult rat cardiomyocytes and mesenchymal stem cells (MSCs) from transgenic GFP positive rats. We investigated in the cocultured MSCs the time course of cellular processes that are difficult to monitor in in vivo experiments. Adult rat cardiomyocytes and adult rat MSCs were cocultured for up to 7 days and analyzed by confocal microscopy. Several markers were studied by immunofluorescence technique. The fluorescent ST-BODIPY-Dihydropyridine was used to label calcium channels in living cells. Intracellular calcium was monitored with the fluorescent probe X-Rhod-1. Immunofluorescence experiments showed the presence of connexin-43 between cardiomyocytes and MSCs and between MSCs, while no sarcomeric structures were observed at any time of the coculture. We looked at the expression of calcium channels and development of voltage-dependent calcium signaling in cocultured MSCs. MSCs showed a time-dependent increase of labeling of ST-BODIPY-Dihydropyridine, reaching a relatively strong level after 72 h of coculture. The treatment with a non-fluorescent DHP, Nifedipine, completely abolished ST-BODIPY labeling. We investigated whether depolarization could modulate intracellular calcium. Depolarization-induced calcium transients increased in MSCs in relation to the coculture time. We conclude that MSCs cocultured with adult cardiomyocytes present preliminary evidence of voltage-dependent calcium modulation uncoupled with the development of nascent or adult myofibrils, thus showing a limited lineage specification and a low plasticity to differentiate in a full cardiomyocyte-like phenotype.

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Mesenchymal stem cells: paradoxes of passaging

Cited by 464 publications

References 185 publications

Migration of dorsal aorta mesenchymal stem cells induced by mouse embryonic circulation

Migration of dorsal aorta mesenchymal stem cells induced by mouse embryonic circulation

Derivation and immunological characterization of mesenchymal stromal cells from human embryonic stem cells

Limited plasticity of mesenchymal stem cells cocultured with adult cardiomyocytes

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