Mesenchymal Stem Cells

Gazit, Zulma; Pelled, Gadi; Sheyn, Dima; Kimelman, Nadav; Gazit, Dan

doi:10.1016/b978-0-12-385942-6.00045-7

Cited by 1 publication

(1 citation statement)

References 240 publications

(179 reference statements)

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“…Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells that exhibit a high potential for pulp regeneration as they can regenerate a dentin/pulp-like structure in vivo (Tatullo et al 2015). hDPSCs have the mesenchymal stem cell (MSCs) characteristics, for example self-renew ability and multipotential differentiation ability toward odontoblast, osteoblast, chondrocytes, adipocyte, and neural cell (Kabir et al 2014;Gazit et al 2013). hDPSCs express numerous MSCs surface markers, including CD29, CD44, CD59, CD73, CD90, CD146, and STRO-1, but these cells do not express surface maker of hematopoietic and endothelial stem cells such as CD14, CD34, CD45, CD133, and CD11b (Rastegar et al 2010).…”

Section: Human Dental Pulp Stem Cellsmentioning

confidence: 99%

The Effects of Decellularized Extracellular Matrix Derived from Jagged1-treated Human Dental Pulp Stem Cells on Biological Responses of Stem Cells Isolated from Apical Papilla

Phothichailert

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Objective: Indirect Jagged1 immobilization efficiently activates canonical Notch signaling in hDPSCs. This study aimed to investigate the characteristic of the Jagged1-treated hDPSCs-derived dECM and its biological activity on odonto/osteogenic differentiation SCAPs. Methods: Bioinformatic database of Jagged1-treated hDPSCs was analyzed by NetworkAnalyst. hDPSCs seeded on Jagged1 immobilized surface were maintained with N�or OM�followed by decellularization procedure, dECM-N or dECM-OM, respectively. SCAPs were reseeded on each dECM with either normal medium or OM. Cell viability was determined by MTT assay. Characteristics of dECMs and SCAPs were evaluated by SEM, EDX, immunofluorescent staining and alcian blue staining. A Multipotential differentiation assay. Statistical significance was considered at p<0.05. Results: RNA-seq database revealed upregulation of several genes involved in ECM organization, ECM-receptor interaction, and focal adhesion in Jagged1-treated hDPSCs. Immobilized Jagged1 increased the osteogenesis of hDPSCs culture with OM. dECMs showed fibrillar-like network structure and major ECM proteins,�as well as glycosaminoglycans. A decrease in calcium and phosphate components was observed in dECMs after the decellularized process. Cell viability on dECMs did not alter by 7 days. Cell attachment and f-actin cytoskeletal organization of SCAPs proliferated on Jagged1-treated dECMs were comparable to those of the control dECMs. SCAPs exhibited significantly higher mineralization on dECM-N in OM and markedly enhanced on dECM-OM with normal medium or OM conditions. Jagged1-treated hDPSCs-derived dECMs have biocompatible and increase odonto/osteogenic differentiation of SCAPs. The results suggested the potential of Jagged1 dECMs, which could be further developed into ECM scaffolds for application in regenerative medicine.

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Section: Human Dental Pulp Stem Cellsmentioning

confidence: 99%

The Effects of Decellularized Extracellular Matrix Derived from Jagged1-treated Human Dental Pulp Stem Cells on Biological Responses of Stem Cells Isolated from Apical Papilla

Phothichailert

View full text Add to dashboard Cite

show abstract

Mesenchymal Stem Cells

Cited by 1 publication

References 240 publications

The Effects of Decellularized Extracellular Matrix Derived from Jagged1-treated Human Dental Pulp Stem Cells on Biological Responses of Stem Cells Isolated from Apical Papilla

The Effects of Decellularized Extracellular Matrix Derived from Jagged1-treated Human Dental Pulp Stem Cells on Biological Responses of Stem Cells Isolated from Apical Papilla

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