Mesenchymal stem cells transplantation reduces diabetic nephropathy

Kazmi, Abeer; Kazmi, Arsheema; Muhammad, Wali; Azhar, Muhammad Naufal

doi:10.15419/psc.v6i1.403

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…Failure of the insulin-producing pancreatic beta cells has been reported in both type 1 and 2 diabetes mellitus (T1D and T2D), which are originating from autoimmune abnormalities or other unknown mechanisms, respectively. Although the promising results of the MSC-based treatments in the T1D and T2D in animal models have promoted several clinical trials (Cho, D'Antuono, Glicksman, Wang, & Jonklaas, 2018), the success rate for the MSC-based regeneration of the pancreatic beta cells is determined by their ROS level, which is under the control of their intrinsic antioxidant system or external antioxidant supplementation (Kazmi, Kazmi, Muhammad, & Azhar, 2019;Kornicka, Houston, & Marycz, 2018;Palová Jelínková, Grohová, Dáňová, & Spisek, 2019).…”

Section: The Therapeutic Efficacy Of Different Antioxidants In Regenerative Medicine: An Overview Of Clinical Trials Involving Mscsmentioning

confidence: 99%

Cytoprotective effects of antioxidant supplementation on mesenchymal stem cell therapy

Panahi

Rahimi

et al. 2020

Journal Cellular Physiology

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Mesenchymal stem cells (MSCs) are earmarked as perfect candidates for cell therapy and tissue engineering due to their capacity to differentiate into different cell types. However, their potential for application in regenerative medicine declines when the levels of the reactive oxygen and nitrogen species (RONS) increase from the physiological levels, a phenomenon which is at least inevitable in ex vivo cultures and air-exposed damaged tissues. Increased levels of RONS can alter the

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Section: The Therapeutic Efficacy Of Different Antioxidants In Regenerative Medicine: An Overview Of Clinical Trials Involving Mscsmentioning

confidence: 99%

Cytoprotective effects of antioxidant supplementation on mesenchymal stem cell therapy

Panahi

Rahimi

et al. 2020

Journal Cellular Physiology

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“…Untargeted SCT supplies stem cells from peripheral circulation so that stem cells should pass lung capillary system to reach damaged organ. Some investigators believe that HSC and MSC have anti-inflammatory and immune-modulatory characteristics in a way that the stem cells find their niche in the damaged organ [ 69 , 70 ]. Thus, the route of stem cell transplantation should be considered as one of the main factors to design a clinical trial for SCT.…”

Section: Discussionmentioning

confidence: 99%

Investigating the safety and efficacy of hematopoietic and mesenchymal stem cell transplantation for treatment of T1DM: a systematic review and meta-analysis

et al. 2022

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Background Stem cell transplantation (SCT) has paved the way for treatment of autoimmune diseases. SCT has been investigated in type 1 diabetes mellitus (T1DM) as an autoimmune-based disorder, but previous studies have not presented a comprehensive view of its effect on treatment of T1DM. Methodology After registration of the present systematic review and meta-analysis in the PROSPERO, a search was done according to the Cochrane guidelines for evaluation of clinical trials to find eligible clinical trials that investigated the effect of SCT on T1DM (based on ADA® diagnostic criteria) from PubMed, Web of science, Scopus, etc, as well as registries of clinical trials from January 1, 2000, to September 31, 2019. A search strategy was designed using MeSH and EM-tree terms. Primary outcome included the changes in the insulin total daily dose (TDD) (U/kg) level, and secondary outcomes included the changes in the HbA1c, c-peptide, and adjusted HbA1c levels. The Q Cochrane test and I2 statistic were performed to assess the heterogeneity and its severity in primary clinical trials. The Cochrane ROB was used to determine risk of bias, and Cochrane Handbook for Systematic Reviews of Interventions was used in the full text papers. The meta-analysis was accomplished in the STATA software, and the results were shown on their forest plots. Confounders were evaluated by the meta-regression test. Results A total of 9452 studies were electronically screened, and 35 papers were included for data extraction. The results of this review study showed that 173 (26.5%) diabetic patients experienced insulin-free period (from 1 to 80 months), and 445 (68%) showed reduction in TDD of insulin after the SCT. Combination of hematopoietic stem cell (HSC) with mesenchymal stem cell (MSC) transplantation were significantly associated with improvement of the TDD (SMD: − 0.586, 95% CI: − 1.204/− 0.509, I2: 0%), HbA1c (SMD: − 0.736, 95% CI: − 1.107/− 0.365, I2: 0%), adjusted HbA1c (SMD: − 2.041, 95% CI: − 2.648/− 1.434, I2: 38.4%), and c-peptide (SMD: 1.917, 95% CI: 0.192/3.641, I2: 92.5%) on month 3 of follow-up, while its association had a growing trend from 3 to 12 months after the transplantation. Considering severe adverse events, HSC transplantation accompanied with conditioning could not be suggested as a safe treatment. Conclusion Most of the clinical trials of SCT in T1DM were single arm. Although meta-analysis illustrated the SCT is associated with T1DM improvement, well-designed randomized clinical trials are needed to clarify its efficacy. Recommendation Based on the results of this meta-analysis, the MSC and its combination with HSC could be considered as “Safe Cell” for SCT in T1DM. Furthermore, to evaluate the SCT efficacy, calculation of insulin TDD (U/kg/day), AUC of c-peptide, and adjusted HbA1c are highly recommended.

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“…MSCs have the ability of self-renewal and differentiation into a variety of cell types. MSCs have fibroblastic appearance with multipotent nature and can be isolated from a wide range of tissues but bone marrow and gingiva are the key sources of MSCs 13,14 . There, MSCs are responsible for the maintenance of the microenvironment (niche) and provide support to HSCs in the bone marrow 15 .…”

Section: Introductionmentioning

confidence: 99%

Therapeutic role of bone marrow-derived stem cells and zinc sulfate for reduction of liver fibrosis

Kazmi

Shams

et al. 2019

Prog. Stem Cell

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Introduction: Hepatic fibrosis is considered as the initial state of any chronic hepatic disease; progression causes accumulation of extracellular matrix (ECM) and develops into cirrhosis. In case of liver failure, transplantation was considered as the only suitable therapy but due to the hurdles in transplantation, therapy using stem cells was promoted. Mesenchymal stem cells (MSCs) are widely used for the treatment of a variety of diseases. Oxidative stress at the damage site causes poor MSC proliferation and engraftment. Thus, it is necessary to induce antioxidants with MSCs to enhance potency. Methods: To explore the therapeutic potential of zinc sulfate (ZnSO4) and MSCs on carbon tetra chloride (CCl4)-induced hepatic toxicity, 6-8 week-old female albino mice (BALB/c) were used. Initially, mice were infected with CCl4 and then intra-peritoneally injected with MSCs only, ZnSO4 only, or a combination of MSCs and ZnSO4. MSCs were isolated from femur and tibia of mice, and were cultured under control conditions. Results: The morphological results revealed that in contrast to MSC-only therapy, ZnSO4 enhanced the therapeutic potential of MSCs when administered to CCl4-injured mice. Biochemically, level of serum Alanine Transaminase (ALT) and total bilirubin was found to be significantly decreased in ZnSO4 + MSC transplanted mice. Histopathological examination also revealed that ZnSO4 + MSC transplantation induced a strong anti-apoptotic effect on CCl4-injured liver. Reverse transcription polymerase chain reaction (RT-PCR) detected a noteworthy anti-fibrotic effect of MSCs in the presence of ZnSO4, down-regulation of apoptotic marker (Bax), and up-regulation of anti-apoptotic (Bcl-xl) and hepatic (Albumin) markers. Conclusion: Thus, it was concluded that the presence of ZnSO4 reduced oxidative stress, enhanced the proliferation rate of MSCs, and significantly attenuated hepatic fibrosis.

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Mesenchymal stem cells transplantation reduces diabetic nephropathy

Cited by 3 publications

References 25 publications

Cytoprotective effects of antioxidant supplementation on mesenchymal stem cell therapy

Cytoprotective effects of antioxidant supplementation on mesenchymal stem cell therapy

Investigating the safety and efficacy of hematopoietic and mesenchymal stem cell transplantation for treatment of T1DM: a systematic review and meta-analysis

Therapeutic role of bone marrow-derived stem cells and zinc sulfate for reduction of liver fibrosis

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