Mesenchymal stem versus stromal cells: International Society for Cell &amp; Gene Therapy (ISCT®) Mesenchymal Stromal Cell committee position statement on nomenclature

Viswanathan, Sowmya; Shi, Yufang; Galipeau, Jacques; Krampera, Mauro; LeBlanc, Katarina; Martin, Ivan; Nolta, Jan A.; Phinney, Donald G.; Sensebé, Luc

doi:10.1016/j.jcyt.2019.08.002

Cited by 561 publications

(472 citation statements)

References 60 publications

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“…1), suggesting that the cells must have undergone e Quantitative analysis of the relative protein expression (n = 3). *P < 0.05, **P < 0.01 vs. the control group; # P < 0.05, ## P < 0.01 vs. the H-EV group some changes in response to inflammation [48,49]. Indeed, the periodontium closely connects to the pulpdentin complex by three interconnected routes, namely, the apical foramen, the dentinal tubules, and the lateral canals, which means that once periodontitis involves a certain tooth, the dental pulp may be negatively affected by bacteria, especially by their byproducts residing in the periodontitis pocket [50,51].…”

Section: Discussionmentioning

confidence: 90%

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

Zhou

Yin

et al. 2020

Stem Cell Res Ther

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Background: Although dental pulp stem cells (DPSCs) isolated from periodontally compromised teeth (P-DPSCs) have been demonstrated to retain pluripotency and regenerative potential, their use as therapeutics remains largely unexplored. In this study, we investigated the proangiogenic effects of extracellular vesicles (EVs) secreted by P-DPSCs using in vitro and in vivo testing models. Methods: Patient-matched DPSCs derived from periodontally healthy teeth (H-DPSCs) were used as the control for P-DPSCs. Conditioned media (CMs) derived from H-DPSCs and P-DPSCs (H-CM and P-CM), CMs derived from both cell types pretreated with the EV secretion blocker GW4869 (H-GW and P-GW), and EVs secreted by H-DPSCs and P-DPSCs (H-EVs and P-EVs) were prepared to test their proangiogenic effects on endothelial cells (ECs). Cell proliferation, migration, and tube formation were assessed using the Cell Counting Kit-8 (CCK-8), transwell/scratch wound healing, and Matrigel assays, respectively. Specifically, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and western blot analysis were used to examine the expression levels of angiogenesis-related genes/proteins in ECs in response to EV-based incubation. Finally, a full-thickness skin defect model was applied to test the effects of EVs on wound healing and new vessel formation.

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Section: Discussionmentioning

confidence: 90%

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

Zhou

Yin

et al. 2020

Stem Cell Res Ther

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“…Nowadays, MSCs can be isolated from a variety of other tissues including periosteum, trabecular bone, adipose tissue, synovial membrane, skin, or skeletal muscles [3]. They can also be found in fetal tissues like the placenta or the conjunctive tissue of the umbilical cord matrix, named Wharton's jelly (WJ) [5]. Although Wharton's jelly mesenchymal stem/stromal cells (WJ-MSCs) share similar characteristics with MSCs from adult tissues, they have higher proliferation potential, can differentiate in a large number of cells [6], and seem more immature based on their immunological properties.…”

Section: Introductionmentioning

confidence: 99%

Are the Immune Properties of Mesenchymal Stem Cells from Wharton’s Jelly Maintained during Chondrogenic Differentiation?

Voisin

Cauchois

Reppel

et al. 2020

JCM

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Background: Umbilical mesenchymal stem/stromal cells (MSCs), and especially those derived from Wharton’s jelly (WJ), are a promising engineering tool for tissue repair in an allogeneic context. This is due to their differentiation capacity and immunological properties, like their immunomodulatory potential and paracrine activity. Hence, these cells may be considered an Advanced Therapy Medicinal Product (ATMP). The purpose of this work was to differentiate MSCs from WJ (WJ-MSCs) into chondrocytes using a scaffold and to evaluate, in vitro, the immunomodulatory capacities of WJ-MSCs in an allogeneic and inflammatory context, mimicked by IFN-γ and TNF-α priming during the chondrogenic differentiation. Methods: Scaffolds were made from hydrogel composed by alginate enriched in hyaluronic acid (Alg/HA). Chondrogenic differentiation, immunological function, phenotype expression, but also secreted soluble factors were the different parameters followed during 28 days of culture. Results: During chondrocyte differentiation, even in an allogeneic context, WJ-MSCs remained unable to establish the immunological synapse or to induce T cell alloproliferation. Moreover, interestingly, paracrine activity and functional immunomodulation were maintained during cell differentiation. Conclusion: These results show that WJ-MSCs remained hypoimmunogenic and retained immunomodulatory properties even when they had undergone chondrocyte differentiation.

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“…The stem cell character of hPDLCs was verified by investigating the expression of mesenchymal and hematopoietic stem cell surface markers (Table ), according to the International Society for Cell and Gene Therapy (Dominici et al, 2006; Viswanathan et al, 2019). Our single‐parameter analysis showed that more than 95% of hPDLCs were positively stained for CD29, CD73, CD90 and CD105.…”

Section: Resultsmentioning

confidence: 99%

“…Human periodontal ligament cells (hPDLCs) fulfil the minimal criteria of mesenchymal stem cells (MSCs) (Seo et al, 2004), including the expression of specific surface markers and a multilineage differentiation potential (Viswanathan et al., 2019). This heterogeneous population of fibroblast‐like cells participates in tissue regeneration by cell proliferation, differentiation into tissue‐specific cells and modulating immune and inflammatory responses (Racz et al, 2014; Wada, Gronthos, & Bartold, 2013; Xiao & Nasu, 2014).…”

Section: Introductionmentioning

confidence: 99%

Pleiotropic effects of vitamin D₃on CD4⁺T lymphocytes mediated by human periodontal ligament cells and inflammatory environment

Behm

Blufstein

Gahn

et al. 2020

J Clinic Periodontology

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Aims: Both, vitamin D 3 and human periodontal ligament cells (hPDLCs) possess immunosuppressive properties, but their combined effect on immune cells has never been investigated. Here, we analysed the impact of vitamin D 3 on the immunosuppressive properties of hPDLCs towards CD4 + T lymphocytes. Material and Methods:Allogenic CD4 + T lymphocytes were activated by phytohemagglutinin either in monoculture or co-culture with hPDLCs, in the presence or absence of IFN-γ and 1,25(OH) 2 D 3 . After 5 days, CD4 + T-lymphocyte proliferation, CD4 + CD25 + FoxP3 + regulatory T lymphocytes (T regs ) proportion and IL-10, TGF-β1 and IL-17A production were analysed. Results:In monoculture, 1,25(OH) 2 D 3 suppressed CD4 + T-lymphocyte proliferation, increased the percentage of CD4 + FoxP3 + CD25 + FoxP3 + T regs and enhanced IL-10 and TGF-β1 production. In the presence of IFN-γ treated hPDLCs, 1,25(OH) 2 D 3 significantly increased CD4 + T-lymphocyte proliferation and decreased the percentage of CD4 + CD25 + FoxP3 + T regs . IL-10 and IL-17A expression was significantly diminished by 1,25(OH) 2 D 3 , whereas TGF-β1 was slightly increased. The effects of 1,25(OH) 2 D 3 in co-culture were reversed by inhibition of indoleamine-2,3-dioxygenase-1, prostaglandin-endoperoxide synthase and programmed cell death 1 ligand 1. 1,25(OH) 2 D 3 also suppressed the expression of these proteins in hPDLCs. Conclusion:Effects of vitamin D 3 on CD4 + T lymphocyte are modified by hPDLCs depending on the microenvironment. K E Y W O R D SCD4-Positive T Lymphocytes, co-culture, Immunomodulation, Periodontal Ligament, Vitamin D

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Mesenchymal stem versus stromal cells: International Society for Cell & Gene Therapy (ISCT®) Mesenchymal Stromal Cell committee position statement on nomenclature

Cited by 561 publications

References 60 publications

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

Are the Immune Properties of Mesenchymal Stem Cells from Wharton’s Jelly Maintained during Chondrogenic Differentiation?

Pleiotropic effects of vitamin D₃on CD4⁺T lymphocytes mediated by human periodontal ligament cells and inflammatory environment

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Mesenchymal stem versus stromal cells: International Society for Cell & Gene Therapy (ISCT®) Mesenchymal Stromal Cell committee position statement on nomenclature

Cited by 561 publications

References 60 publications

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

Are the Immune Properties of Mesenchymal Stem Cells from Wharton’s Jelly Maintained during Chondrogenic Differentiation?

Pleiotropic effects of vitamin D3on CD4+T lymphocytes mediated by human periodontal ligament cells and inflammatory environment

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Pleiotropic effects of vitamin D₃on CD4⁺T lymphocytes mediated by human periodontal ligament cells and inflammatory environment