Mesenchymal stromal cell injection protects against oxidative stress in Escherichia coli–induced acute lung injury in mice

Shalaby, Sally M.; El‐Shal, Amal S.; Abd-Allah, Somia H.; Selim, Assmaa O.; Selim, Sally A.; Gouda, Zienab A.; Motteleb, Dalia M. Abd El; Zanfaly, Hala E; EL-Assar, Heba M.; Abdelazim, Shymaa

doi:10.1016/j.jcyt.2013.12.006

Cited by 59 publications

(51 citation statements)

References 41 publications

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“…LPS group, #vs. LPS+ MSCs group (one-way ANOVA followed by Tukey-Kramer multiple comparisons test; p < 0.05).Where, BM-MSCs; bone marrow mesenchymal stem cells, LPS; lipopolysaccharide, Dex; dexamethasone, NaHCO 3 ; sodium bicarbonate. This data is in line with the finding of Shalaby et al, (2014) who reported an increased survival rate, reduced pulmonary edema and attenuated lung injuries in ALI mice through an increase of anti-oxidant enzyme activities and a decrease of myeloperoxidase activity and malondialdehyde levels in the MSC recipient groups versus the ALI group. These was accompanied by weak +ve inducible nitric oxide synthase immuno-expression in that groups received MSCs tratment.…”

Section: Oxidative Stress Biomarkers In Lung Tissuesupporting

confidence: 92%

Impact of Mesenchymal Stem Cells on Lipopolysaccharide-Induced Apoptosis in Acute Lung Injury Rat Model

Mansour¹,

Soliman²,

Hasan³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Section: Oxidative Stress Biomarkers In Lung Tissuesupporting

confidence: 92%

Impact of Mesenchymal Stem Cells on Lipopolysaccharide-Induced Apoptosis in Acute Lung Injury Rat Model

Mansour¹,

Soliman²,

Hasan³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…Thus, treatment with CD34þ cells significantly decreased the pulmonary edema and improved the lung wet/dry ratio. Likewise, pulmonary edema is relieved after administration of MSCs derived from bone marrow [4,9] or adipose tissue [35]. We also found that CD34þ cells transplantation reduces hypoxemia at 6 and 24 h after OA challenge.…”

Section: Discussionsupporting

confidence: 53%

“…Harvesting a patient's bone marrow to isolate and culture autologous CD34þ cells cannot be achieved quickly enough to provide emergency treatment for acute disease such as ALI. Therefore, human peripheral blood may be an ideal and practical source for HSCs (such as CD34þ cells and MSCs) because of its accessibility (they do not require invasive bone marrow biopsies), lower risk of viral contamination, immunosuppressive properties and homing to sites of tissue injury [9].…”

Section: Discussionmentioning

confidence: 99%

Human peripheral blood CD34+ cells attenuate oleic acid–induced acute lung injury in rats

et al. 2015

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“…MSCs are capable of selfrenewal and can be obtained from multiple sources, including bone marrow, adipose tissue, umbilical cord blood, skin, tendon, muscle, and dental pulp [6,[12][13][14]. Their relative ease of isolation and enormous expansion potential in culture make them attractive therapeutic candidates [15]. Bone marrow-derived mesenchymal stem cells (BM-MSCs) are one of several types of stem cells, and considerable evidence has demonstrated that BM-MSCs hold promise for the treatment of ALI/ARDS [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cells Modified with Heme Oxygenase-1 Have Enhanced Paracrine Function and Attenuate Lipopolysaccharide-Induced Inflammatory and Oxidative Damage in Pulmonary Microvascular Endothelial Cells

Chen

Zhang

Wang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation has therapeutic effects on endothelial damage during acute lung injury (ALI). Heme oxygenase-1 (HO-1) can restore homeostasis and implement cytoprotective defense functions in many pathologic states. Therefore, we explored whether transduction of HO-1 into BM-MSCs (MSCs-HO-1) would have an increased beneficial effect on lipopolysaccharide (LPS)-induced inflammatory and oxidative damage in human pulmonary microvascular endothelial cells (PVECs). Methods: MSCs were isolated from rat bone marrow and transfected with the HO-1 gene by a lentivirus vector. The phenotype and multilineage differentiation of MSCs were assessed. MSCs or MSCs-HO-1 were co-cultured with PVECs using a transwell system, and LPS was added to induce PVEC injury. The production of reactive oxygen species (ROS), and the activities of lipid peroxide (LPO), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in PVECs were determined by flow cytometry and colorimetric assays, respectively. The levels of human PVEC-derived tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in the supernatants of the co-culture system, and the activity of nuclear transcription factor-κB and NF-E2-related factor 2 (Nrf2) in PVECs were examined by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of TNF-α, IL-1β and IL-6 in PVECs was detected by quantitative real-time polymerase chain reaction (qRT-PCR), HO-1 expression and enzymatic activity in PVECs and the influence of zinc protoporphyrin (ZnPP) or HO-1 small interfering RNA on the above inflammatory and oxidative stress markers were evaluated. In addition, the expression of rat MSC-derived hepatocyte growth factor (HGF) and IL-10 was determined by ELISA and qRT-PCR. Results: MSCs showed no significant changes in phenotype or multilineage differentiation after transduction. LPS strongly increased the production of inflammatory and oxidative stress indicators, as well as decreased the levels of antioxidant components and the activity of Nrf2 in PVECs. MSC co-cultivation ameliorated these detrimental effects in PVECs and MSCs-HO-1 further improved the damage to PVECs induced by LPS when compared with MSCs alone. The beneficial effects of MSCs-HO-1 were dependent on HO-1 overexpression and may be attributed to the enhanced paracrine production of HGF and IL-10. Conclusion: MSCs-HO-1 have an enhanced ability to improve LPS-induced inflammatory and oxidative damage in PVECs, and the mechanism may be partially associated with the enhanced paracrine function of the stem cells. These data encourage further testing of the beneficial effects of MSCs-HO-1 in ALI animal models.

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Mesenchymal stromal cell injection protects against oxidative stress in Escherichia coli–induced acute lung injury in mice

Cited by 59 publications

References 41 publications

Impact of Mesenchymal Stem Cells on Lipopolysaccharide-Induced Apoptosis in Acute Lung Injury Rat Model

Impact of Mesenchymal Stem Cells on Lipopolysaccharide-Induced Apoptosis in Acute Lung Injury Rat Model

Human peripheral blood CD34+ cells attenuate oleic acid–induced acute lung injury in rats

Mesenchymal Stem Cells Modified with Heme Oxygenase-1 Have Enhanced Paracrine Function and Attenuate Lipopolysaccharide-Induced Inflammatory and Oxidative Damage in Pulmonary Microvascular Endothelial Cells

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