Mesonia aestuariivivens sp. nov., isolated from a tidal flat

Abstract

A Gram-negative, aerobic, non-agellated and ovoid or rod-shaped bacterial strain (JHPTF-M18 T ), which was isolated from a tidal at sediment in Republic of Korea, was taxonomically characterized. 16S rRNA gene sequence analysis showed that strain JHPTF-M18 T forms phylogenetic lineage within the radiation comprising type strains of Mesonia species. The 16S rRNA gene of strain JHPTF-M18 T shared sequence similarities of 97.7% with that of type strain of M. mobilis and 92.9-96.8% with those of type strains of eight other Mesonia species. The DNA G+C content was 33.1% based on its genomic sequence. ANI and dDDH values between strain JHPTF-M18 T and the type strains of M. mobilis, M. oceanica, M. phycicola and M. algae were 73.1-79.7% and 18.5-22.8%, respectively. Strain JHPTF-M18 T contained MK-6 as the predominant menaquinone and iso-C 15:0 , iso-C 17:0 3-OH and summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c) as its major fatty acids. Major polar lipids of strain JHPTF-M18 T were phosphatidylethanolamine and two unidenti ed lipids. Strain JHPTF-M18 T was separated from recognized Mesonia species by its phenotypic properties together with the phylogenetic and genetic distinctiveness. Based on data presented in this study, strain JHPTF-M18 T is considered to represent a novel species of the genus Mesonia. The name Mesonia aestuariivivens sp. nov. is proposed for JHPTF-M18 T (= KACC 22185 T = NBRC 115119 T ).

Characteristics and Application of a Novel Cold-Adapted and Salt-Tolerant Protease EK4-1 Produced by an Arctic Bacterium Mesonia algae K4-1

“…The genus Mesonia was created by Nedashkovskaya et al with Mesonia algae as the type species and belonged to the family Flavobacterium of the phylum Bacteroidetes. The genus Mesonia currently consists of ten species with validly published names, as of July 2022 (https://lpsn.dsmz.de/genus/mesonia (accessed on 10 March 2023)) [12]. In this study, the major extracellular protease EK4-1 secreted by Mesonia algae K4-1 was isolated and purified, and its cold adaptation and salt tolerance properties were analyzed based on the amino acid composition and three-dimensional structure characteristics.…”