Mesophilic Fermentation of Renewable Biomass: Does Hydraulic Retention Time Regulate Methanogen Diversity?

Krakat, Niclas; Schmidt, Štefan; Scherer, Paul

doi:10.1128/aem.00927-10

Cited by 37 publications

(21 citation statements)

References 47 publications

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“…Methanogenic Archaea are in the focus of many studies because they play a key role in the biogas cascade, methanogenesis, although their abundance and diversity are significantly lower than those of the Bacteria [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Biphasic Study to Characterize Agricultural Biogas Plants by High-Throughput 16S rRNA Gene Amplicon Sequencing and Microscopic Analysis

Maus

Kim

Wibberg

et al. 2017

Journal of Microbiology and Biotechnology

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Section: Introductionmentioning

confidence: 99%

Biphasic Study to Characterize Agricultural Biogas Plants by High-Throughput 16S rRNA Gene Amplicon Sequencing and Microscopic Analysis

Maus

Kim

Wibberg

et al. 2017

Journal of Microbiology and Biotechnology

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“…Furthermore, anaerobic fungi are known to form co-cultures with ruminal methanogenic archaea which utilize the fungal hydrogen production [13]. The role of ruminal fungi in the degradation of plant fibres has been studied extensively [9,10,[14][15][16][17]. The fungi can attach to the most lignified plant tissues [18] and are in turn followed by the ingress of cellulolytic bacteria which then gain access to the interior of otherwise less fermentable plant material.…”

Section: Introductionmentioning

confidence: 99%

Fungi open new possibilities for anaerobic fermentation of organic residues

2014

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Background: Large amounts of fibre-rich organic waste material from public green and private gardens have to be treated environmentally friendly; however, this fibre-rich biomass has low biogas yields. This study investigated the presence of fungi in full-scale biogas plants as well as in laboratory reactors and elucidated the importance of fungi for the biogas process. Methods: The dominating members of the eukaryotic community were identified by analyzing 18S rRNA gene and internal transcribed spacer 1 (ITS1) region fragments of clone libraries. These identifications were accompanied by diverse microscopic techniques such as fluorescence microscopy and conventional scanning electron microscopy. Results: Cells of presumably fungal origin were characterized by intensive fluorescence and were about 1 order of magnitude larger than prokaryotic cells. Molecular techniques enabled to identify fungi from the subphyla Agaricomycotina, Mucoromycotina, Pezizomycotina, Pucciniomycotina and Saccharomycotina and from the class Neocallimastigomycetes. Members of these groups can be important for microbial degradation of complex compounds, due to the ability to penetrate cell walls, and thus open the cells for the influx of bacteria, further enhancing degradation.

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“…NMDS analysis of biodiversity data, which is a powerful tool to study the spatiotemporal microbial population dynamics, has been applied for different microbial communities, such as those in compost (47), anaerobic sludge (30), and mangrove roots (9). In this study, the NMDS approach to the PCR-DGGE and quinone-profile data sets revealed a marked population shift in the low Mw PLLA-acclimated and PHBVacclimated reactors.…”

Section: Discussionmentioning

confidence: 98%

Nitrate Removal Efficiency and Bacterial Community Dynamics in Denitrification Processes Using Poly (<sc>L</sc>-lactic acid) as the Solid Substrate

et al. 2011

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Laboratory-scale solid-phase denitrification (SPD) reactors for nitrate removal were constructed by acclimating activated sludge with poly (L-lactic acid) (PLLA) having weight-average molecular weights (Mw) of 9,900, 12,000, and 45,100 g mol −1 . A good nitrate removal rate (3.5-5.3 mg NO3 − -N g [dry wt] −1 h −1 ) was found in the reactor containing PLLA of 9,900 g mol −1 , whereas the other two reactors with the higher Mw PLLA showed low nitrate removal efficiency. Microbial community dynamics in the low Mw PLLA-acclimated reactor were studied by 16S rRNA gene-targeted PCR-denaturing gradient gel electrophoresis and quinone profiling. Nonmetric multidimensional scaling analyses of these data sets revealed a marked population shift during acclimation of the SPD reactor with low Mw PLLA. The 16S rRNA gene clone library and culture-dependent analyses showed that bacteria belonging to the family Comamonadaceae predominated and played the primary role in denitrification in the PLLA-using reactor; however, none of the bacterial isolates from the reactor degraded PLLA. These results suggest that the nitrate removal property of the PLLA-using SPD reactor is attained through the bioavailability of hydrolysates released abiotically from the solid substrate.

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Mesophilic Fermentation of Renewable Biomass: Does Hydraulic Retention Time Regulate Methanogen Diversity?

Cited by 37 publications

References 47 publications

Biphasic Study to Characterize Agricultural Biogas Plants by High-Throughput 16S rRNA Gene Amplicon Sequencing and Microscopic Analysis

Biphasic Study to Characterize Agricultural Biogas Plants by High-Throughput 16S rRNA Gene Amplicon Sequencing and Microscopic Analysis

Fungi open new possibilities for anaerobic fermentation of organic residues

Nitrate Removal Efficiency and Bacterial Community Dynamics in Denitrification Processes Using Poly (<sc>L</sc>-lactic acid) as the Solid Substrate

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