Mesophilic inoculation enhances primary and secondary copper sulfide bioleaching altering the microbial &amp; mineralogical ore dynamics

Bobadilla-Fazzini, Roberto A.; Piña, Patricia; Gautier, V.; Brunel, Karen; Parada, Pilar

doi:10.1016/j.hydromet.2016.07.016

Cited by 9 publications

(2 citation statements)

References 21 publications

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“…Also noteworthy is the effect of microorganisms to mineral bioleaching. The microbiology of leaching environments-including physiology of the most common community members, microbial successions [7,58], the relationship of the population dynamics with environmental factors [59,60], and the influence of community composition on ecosystem functioning [61,62]-have always been the target for research on bioleaching processes and mechanisms. Next generation sequencing technology enables the comprehensive analysis of genomes, which can recover information about their general characteristics, especially their metabolic potential [3,63].…”

Section: Ngs For Genome Analysismentioning

confidence: 99%

Assessment of Bioleaching Microbial Community Structure and Function Based on Next-Generation Sequencing Technologies

et al. 2018

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It is widely known that bioleaching microorganisms have to cope with the complex extreme environment in which microbial ecology relating to community structure and function varies across environmental types. However, analyses of microbial ecology of bioleaching bacteria is still a challenge. To address this challenge, numerous technologies have been developed. In recent years, high-throughput sequencing technologies enabling comprehensive sequencing analysis of cellular RNA and DNA within the reach of most laboratories have been added to the toolbox of microbial ecology. The next-generation sequencing technology allowing processing DNA sequences can produce available draft genomic sequences of more bioleaching bacteria, which provides the opportunity to predict models of genetic and metabolic potential of bioleaching bacteria and ultimately deepens our understanding of bioleaching microorganism. High-throughput sequencing that focuses on targeted phylogenetic marker 16S rRNA has been effectively applied to characterize the community diversity in an ore leaching environment. RNA-seq, another application of high-throughput sequencing to profile RNA, can be for both mapping and quantifying transcriptome and has demonstrated a high efficiency in quantifying the changing expression level of each transcript under different conditions. It has been demonstrated as a powerful tool for dissecting the relationship between genotype and phenotype, leading to interpreting functional elements of the genome and revealing molecular mechanisms of adaption. This review aims to describe the high-throughput sequencing approach for bioleaching environmental microorganisms, particularly focusing on its application associated with challenges.

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Section: Ngs For Genome Analysismentioning

confidence: 99%

Assessment of Bioleaching Microbial Community Structure and Function Based on Next-Generation Sequencing Technologies

et al. 2018

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“…Several works have described the diversity of species inhabiting bioleaching systems and acid mine drainage environments and their relevance in the metal recovery process (Bond and Banfield, 2001; Demergasso et al, 2005; Xie et al, 2007; Remonsellez et al, 2009; Bobadilla-Fazzini et al, 2017). Leptospirillum ferriphilum is a Gram-negative and extreme acidophilic bacterium that can derive energy only from ferrous iron oxidization and can only use oxygen as final electron acceptor (Coram and Rawlings, 2002).…”

Section: Introductionmentioning

confidence: 99%

Validation of Genetic Markers Associated to Oxygen Availability in Low-Grade Copper Bioleaching Systems: An Industrial Application

et al. 2019

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Forced aeration is one of the major energy consumption factors of the bioleaching process of run-of-mine ore. The effect of aeration in the microbial community has scarcely been studied at industrial level. Leptospirillum ferriphilum is one of the most representative species of the Fe 3+ producing population in this kind of systems. We analyzed the effect of oxygen availability on L. ferriphilum by growth activity and transcriptional dynamics of its two terminal oxidases (cbb3 and bd complexes) under different experimental test: culture reactor, bioleaching column, and industrial heap tests. Relatively low O 2 availability triggered important changes in the microbial community composition, cell growth, microbial activity and cydAB genes transcription in all cases of study. We assessed the potential role of the terminal oxidases on the adaptation to variable aeration conditions in different lifestyles of L. ferriphilum and identified transcriptional markers associated to oxygen metabolism in an industrial system. An interesting hypothesis about the possible role of the cbb3 complex in the response to oxidative stress as well as their role as a high oxygen-affinity oxidase in L. ferriphilum is proposed and discussed. This study successfully proves the function of the cydAB genes as valid genetic markers for low-grade copper industrial bioleaching systems.

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Characterization and uranium bioleaching performance of mixed iron- and sulfur-oxidizers versus iron-oxidizers

Sun

Ding

et al. 2017

J Radioanal Nucl Chem

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Mesophilic inoculation enhances primary and secondary copper sulfide bioleaching altering the microbial & mineralogical ore dynamics

Cited by 9 publications

References 21 publications

Assessment of Bioleaching Microbial Community Structure and Function Based on Next-Generation Sequencing Technologies

Assessment of Bioleaching Microbial Community Structure and Function Based on Next-Generation Sequencing Technologies

Validation of Genetic Markers Associated to Oxygen Availability in Low-Grade Copper Bioleaching Systems: An Industrial Application

Characterization and uranium bioleaching performance of mixed iron- and sulfur-oxidizers versus iron-oxidizers

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