Mesophyll distribution of ‘antioxidant’ flavonoid glycosides in Ligustrum vulgare leaves under contrasting sunlight irradiance

Agati, Giovanni; Stefano, Giovanni; Biricolti, Stefano; Tattini, Massimiliano

doi:10.1093/aob/mcp177

Cited by 170 publications

(124 citation statements)

References 57 publications

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“…Flavonoids indeed may protect membranes by enhancing membrane rigidity and therefore preserving them against oxidative damage [38,39]. In our case, there was no vacuolar fluorescence in the palisade cells of the grapevine, while Agati [40,41] successfully detected NS-conjugated flavonoids in the vacuoles of mesophyll cells in Ligustrum vulgare under 100% natural sunlight, but failed to visualize flavonoids in other cellular compartments. They detected light-responsive quercetin and luteolin derivatives, while hydroxycinnamates, which serve specific UV-B screening functions [42], and monohydroxy-B-ring flavonoids were unresponsive to excess light.…”

Section: Changes In Leaf Flavonoid Distributionmentioning

confidence: 56%

Main Leaf Polyphenolic Components of Berry Color Variant Grapevines and Their Acclimative Responses to Sunlight Exposure

Kocsis

Abrankó²,

Ayaydin³

et al. 2015

Applied Sciences

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Grapevine leaf synthesizes a wide variety of bioactive secondary metabolites, including polyphenols, which are also key components in ensuring development and growth of the whole plant even under adverse environmental conditions. Our study evaluates the nonanthocyanin polyphenolic composition in grapevine leaves of three varieties of Gohér conculta (Vitis vinifera L.) native to Hungary. A high performance liquid chromatography (HPLC) system including a diode array detector (DAD) coupled to a time-of-flight mass spectrometer (q-TOFMS) was successfully applied to profile intact glycoconjugate forms in samples. In-source fragmentation was utilized in order to provide structural information on the compounds. Using this method, the presence of 16 polyphenolic metabolites were confirmed, and eight of them were subjected to further quantification in sun acclimated and half shaded leaves. Intracellular microimaging detected accumulation of flavonols in cell nuclei, cell wall and chloroplasts. Our findings demonstrated that Gohér conculta-a special grapevine taxon of our viticultural heritage with berry color variants-is a suitable model to OPEN ACCESSAppl. Sci. 2015Sci. , 5 1956 study the interaction between genetic and environmental factors in determination of grapevine phenolic composition.

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Section: Changes In Leaf Flavonoid Distributionmentioning

confidence: 56%

Main Leaf Polyphenolic Components of Berry Color Variant Grapevines and Their Acclimative Responses to Sunlight Exposure

Kocsis

Abrankó²,

Ayaydin³

et al. 2015

Applied Sciences

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“…Under realistic spectral regimes in field experiments there is a species-specific plant response, including alterations in plant morphology and architecture (Kakani et al, 2003), DNA damage and antioxidant response (Hollósy, 2002), and an effect on photosynthetic efficiency (Burger & Edwards, 1996). Still, perhaps the most common plant response to ambient and elevated UV-B radiation is the production of UV-absorbing compounds, such as phenolic compounds (Agati et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Effect of Ambient Sunlight Intensity on the Temporal Phenolic Profiles of Vitis Vinifera L. Cv. Chardonnay During the Ripening Season – A Field Study

Šebela

Turóczy

Olejníčková

et al. 2017

SAJEV

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Grape phenolics are considered to have a significant impact on wine quality, with their quantity and dynamics being strongly influenced by environmental conditions. We investigated the effect of ambient sunlight on the temporal dynamics of phenolics in cv. Chardonnay under field conditions during the 2012 ripening season, from véraison until harvest. The phenolic profiles of the grapes were monitored at predefined time intervals by using high-pressure liquid chromatography (HPLC) and spectrophotometric (OD) analysis. The obtained concentrations were correlated with the average sunlight intensities preceding the sampling. No significant correlations were found between the hydroxycinnamic acid, hydroxybenzoic acid and stilbene content, in contrast with the strong relationships found between: (i) OD 280 nm , (ii) catechin, (iii) flavanols and (iv) total polyphenols and the fluctuating dose of ambient sunlight reaching the grapes throughout the ripening season. The light-dependent dynamics of several main phenolic compounds in cv. Chardonnay during the ripening period could help to establish correlation models that increase the applicability of meteorological data in the assessment of optimal phenolic ripeness in modern viticulture.

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“…In broad-leaved plant species the chloroplasts of the mesophyll tissues are readily protected against UV-radiation by yellow compounds from the flavonol group [7,8]. For example, quercetin derivatives as wide-spread flavonols can be bound to proteins, so triggering significant antioxidant effects against oxygen-induced cytotoxicity [9,10].…”

Section: Introductionmentioning

confidence: 99%

Acclimation Changes of Flavonoids in Needles of Conifers during Heat and Drought Stress 2015

Feucht

Schmid

Treutter

2016

Climate

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Abstract:The long-term harsh climate conditions in 2015 distorted already from June up to November in all study trees of Tsuga and Taxus the intracellular organization of the needles. Intimately involved in these repressive processes were the flavanols, a small subgroup of the flavonoids. They were not only deposited in vacuoles of conifer needles but also in the nuclei and chromosomes. Among the many flavonoids the small group of catechin derivatives and polymers named flavanols can exclusively be stained blue with DMACA (dimethylaminocinnamaldehyde). From mid-July onward, the vacuolar flavanols of the epidermal cell layers were gradually diminished as evidenced by decreasing blue staining of nuclei and vacuoles. Subsequently, in August also the large spongy mesophyll cells showed the flavanols decreasing progressively. Apparently, the antioxidant flavanols operate as oxygen radical scavengers. (ROS) were used up during the harsh environmental stress conditions. Both, Tsuga and Taxus reacted in this way. However, it is quite surprising that in all study trees the palisade cells did not contain such vacuolar flavanols. Only these cells were in June the first to show a loss of chlorophyll from chloroplasts as well as an efflux of flavanols from the nuclei. Conversely, from September onward another group of phenols, the yellow-staining flavanols were newly formed in the palisade cells and later on also in the mesophyll cells. Obviously, they were assembled finally to stabilize finally the fragile cell sites. Summing up, the present study shows by cytological studies that the climatic conditions in 2015 produced the worst disturbance of subcellular structures observed since 2000 when our studies on nuclear phenols in needles of conifers were initiated.

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Mesophyll distribution of ‘antioxidant’ flavonoid glycosides in Ligustrum vulgare leaves under contrasting sunlight irradiance

Abstract: The above findings lead to the hypothesis that flavonoids play a key role in countering light-induced oxidative stress, and not only in avoiding the penetration of short solar wavelengths in the leaf.

Cited by 170 publications

References 57 publications

Main Leaf Polyphenolic Components of Berry Color Variant Grapevines and Their Acclimative Responses to Sunlight Exposure

Main Leaf Polyphenolic Components of Berry Color Variant Grapevines and Their Acclimative Responses to Sunlight Exposure

Effect of Ambient Sunlight Intensity on the Temporal Phenolic Profiles of Vitis Vinifera L. Cv. Chardonnay During the Ripening Season – A Field Study

Acclimation Changes of Flavonoids in Needles of Conifers during Heat and Drought Stress 2015

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