Mesophyll-localized phytochromes gate stress- and light-inducible anthocyanin accumulation in<i>Arabidopsis thaliana</i>

Oh, Sookyung; Warnasooriya, Sankalpi N.; Montgomery, Beronda L.

doi:10.4161/psb.28013

Cited by 11 publications

(10 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We initially proposed to increase the anthocyanin content of purple carrot roots and achieving high root yields by reducing N application to 33% of the recommended level and by extending the harvesting period towards the Central European winter. Indeed, later harvests increased the anthocyanin concentration in both carrot leaves and roots, albeit there were no effects due to the reduced N supply which contradicts other studies (e.g., [23]). The root yield was neither significantly affected by later harvests nor by reduced N supply.…”

Section: Discussioncontrasting

confidence: 85%

“…As the N concentrations in roots and leaves of carrots from all treatments ( Figure 2) were within the range of those reported for orange carrot varieties [18,37], they were not a limiting factor for plant performance. Thus, a reduced N supply did not result in increased synthesis of anthocyanins in leaves and roots (Figure 4), contrary to our expectations based on reports for various other plant species [15,[22][23][24][25][26]. It was shown that the concentration of phenolic compounds increased in well-fertilized compared to non-fertilized orange carrot plants [29] suggesting a positive relationship of N supply and concentration of phenolics in carrots.…”

Section: Reduced N Supply Has Little Effects On Purple Carrot Yield Acontrasting

confidence: 91%

“…However, the results of the non-invasive measurements are not entirely reflected by the chemical analyses where the anthocyanin content increased when the carrots were fertilized with the recommended N level, but had no effects under reduced N supply (Figure 4c). This is surprising as the N limitation in combination with low temperatures is known to increase the concentration of anthocyanins in leaves [23], but this was apparently not true for carrot roots.…”

Section: Later Harvests Do Not Increase the Yield But Alter The Root mentioning

confidence: 97%

“…Indeed, for a wide range of plant species, including model plants such as Arabidopsis thaliana [15,23,24] and agricultural or horticultural crops [24][25][26], it was shown that N deficiency results in the accumulation of foliar anthocyanins. For orange carrots, Seljasen et al [8,27] proposed a relatively low level of N fertilization, having benefits for their composition and their sensory properties.…”

Section: Introductionmentioning

confidence: 99%

“…Extending the harvest period of purple carrots from autumn into early winter, when light intensity is still relatively high while the temperatures drop, may, thus, enhance the accumulation of anthocyanins in the roots. The combination of low temperatures and N limitation is known to stimulate the accumulation of anthocyanins [23]. Moreover, a prolonged growth period may increase root yield as well [30].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Do Extended Cultivation Periods and Reduced Nitrogen Supply Increase Root Yield and Anthocyanin Content of Purple Carrots?

et al. 2018

View full text Add to dashboard Cite

Purple carrots are rich in anthocyanins which are interesting as natural dyes in food and beverages. It is, thus, relevant to increase the concentration of anthocyanins by agricultural practices. We tested whether the combination of reduced nitrogen (N) supply and extended harvesting periods maximized the anthocyanin concentration of purple carrot roots, ideally without reducing their yield. The carrot variety 'Deep Purple' was grown with total N supplies of 220 kg N ha −1 (controls) and 73 kg N ha −1 (reduced N), respectively. Upon harvests in September, October and November, root yield and quality were assessed. Concentrations of chlorophylls (leaves) and anthocyanins (roots and leaves) were determined by spectroscopic and chemical analyses, and carbon and N content were quantified. Reduced N supply neither affected leaf or root biomass nor their chemical composition. Later harvests did not impact the yield of roots, but increased their diameter by 8.5-20%. Additionally, the anthocyanin concentrations of the roots increased by 40-50% in the controls, but not in N-limited plants, at late harvests. Consequently, extending the harvesting period might increase the anthocyanin concentration in roots of 'Deep Purple'. Moreover, N supply might be reduced for this carrot variety without negative effects on root yield.

show abstract

Section: Discussioncontrasting

confidence: 85%

Section: Reduced N Supply Has Little Effects On Purple Carrot Yield Acontrasting

confidence: 91%

Section: Later Harvests Do Not Increase the Yield But Alter The Root mentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Do Extended Cultivation Periods and Reduced Nitrogen Supply Increase Root Yield and Anthocyanin Content of Purple Carrots?

et al. 2018

View full text Add to dashboard Cite

show abstract

Overexpression of LOV KELCH PROTEIN 2 confers dehydration tolerance and is associated with enhanced expression of dehydration-inducible genes in Arabidopsis thaliana

et al. 2015

View full text Add to dashboard Cite

The overexpression of LKP2 confers dehydration tolerance in Arabidopsis thaliana ; this is likely due to enhanced expression of dehydration-inducible genes and reduced stomatal opening. LOV KELCH protein 2 (LKP2) modulates the circadian rhythm and flowering time in plants. In this study, we observed that LKP2 overexpression enhanced dehydration tolerance in Arabidopsis. Microarray analysis demonstrated that expression of water deprivation-responsive genes was higher in the absence of dehydration stress in transgenic Arabidopsis plants expressing green fluorescent protein-tagged LKP2 (GFP-LKP2) than in control transgenic plants expressing GFP. After dehydration followed by rehydration, GFP-LKP2 plants developed more leaves and roots and exhibited higher survival rates than control plants. In the absence of dehydration stress, four dehydration-inducible genes, namely DREB1A, DREB1B, DREB1C, and RD29A, were expressed in GFP-LKP2 plants, whereas they were not expressed or were expressed at low levels in control plants. Under dehydration stress, the expression of DREB2B and RD29A peaked faster in the GFP-LKP2 plants than in control plants. The stomatal aperture of GFP-LKP2 plants was smaller than that of control plants. These results suggest that the dehydration tolerance of GFP-LKP2 plants is caused by upregulation of DREB1A-C/CBF1-3 and their downstream targets; restricted stomatal opening in the absence of dehydration stress also appears to contribute to the phenotype. The rapid and high expression of DREB2B and its downstream target genes also likely accounts for some features of the GFP-LKP2 phenotype. Our results suggest that LKP2 can be used for biotechnological applications not only to adjust the flowering time control but also to enhance dehydration tolerance.

show abstract

Gene Expression Characteristics in Response to Abscisic Acid Under Shade

et al. 2021

View full text Add to dashboard Cite

Mesophyll-localized phytochromes gate stress- and light-inducible anthocyanin accumulation inArabidopsis thaliana

Cited by 11 publications

References 40 publications

Do Extended Cultivation Periods and Reduced Nitrogen Supply Increase Root Yield and Anthocyanin Content of Purple Carrots?

Do Extended Cultivation Periods and Reduced Nitrogen Supply Increase Root Yield and Anthocyanin Content of Purple Carrots?

Overexpression of LOV KELCH PROTEIN 2 confers dehydration tolerance and is associated with enhanced expression of dehydration-inducible genes in Arabidopsis thaliana

Gene Expression Characteristics in Response to Abscisic Acid Under Shade

Contact Info

Product

Resources

About