Mesoporous Hollow Manganese Doped Ceria Nanoparticle for Effectively Prevention of Hepatic Ischemia Reperfusion Injury

Abstract

Introduction Hepatic ischemia-reperfusion injury (HIRI) is the main reason for liver dysfunction or failure after liver resection and liver transplantation. As excess accumulation of reactive oxygen species (ROS) is the leading factor, ceria nanoparticle, a cyclic reversible antioxidant, is an excellent candidate for HIRI. Methods Manganese doped mesoporous hollow ceria nanoparticles (MnO x -CeO 2 NPs) were prepared, and the physicochemical characteristics, such as particle size, morphology, microstructure, etc. were elucidated. The in vivo safety and liver targeting effect were examined after i.v. injection. The anti-HIRI was determined by a mouse HIRI model. Results MnO x -CeO 2 NPs with 0.40% Mn doped exhibited the strongest ROS-scavenging capability, which may due to the increased specific surface area and surface oxygen concentration. The nanoparticles accumulated in the liver after i.v. injection and exhibited good biocompatibility. In the HIRI mice model, MnO x -CeO 2 NPs significantly reduced the serum ALT and AST level, decreased the MDA level and increased the SOD level in the liver, prevent pathological damages in the liver. Conclusion MnO x -CeO 2 NPs were successfully prepared and it could significantly inhibit the HIRI after i.v. injection.

Reactive Oxygen Species Scavenging Nanozymes: Emerging Therapeutics for Acute Liver Injury Alleviation

“…In order to better study the mechanism and treatment of acute liver injury, different kinds of acute liver injury animal models have been established, including drug-induced liver injury (eg APAP-induced liver injury, [58][59][60] anthracyclineinduced liver injury 61 ), chemical-induced liver injury (eg CCl 4 -induced liver injury 62,63 ), immune-induced liver injury (eg lipopolysaccharide (LPS)-induced liver injury, 64,65 D-galactosamine-induced liver injury, 66 concanavalin A-induced liver injury 67,68 ), alcohol-induced liver injury, 69,70 and hepatic ischemia-reperfusion injury. 7,35,71 Transition Metal-Based Nanozymes…”

“…These nanoparticles were further investigated for their potential in vivo application for inhibiting HIRI. 71 The administration of MnO x -CeO 2 NPs led to a notable decline in serum levels of alanine transaminase (ALT) and aspartate transaminase (AST), a reduction in malondialdehyde (MDA) level, an elevation in superoxide dismutase (SOD) level in the liver, and a mitigation of the pathological alterations induced by HIRI.…”

“… 80 Manganese-based composite nanozymes, such as mesoporous hollow doped ceria nanoparticles (MnOx-CeO 2 NPs) and BSA-functionalized CeO 2 and MnO 2 composite nanoparticles (CM NCs), also have been developed to alleviate the hepatic ischemia-reperfusion injury ( Figures 2C ). 71 , 75 The CM NCs can scavenge and eliminate •O 2 − , H 2 O 2 , and •OH during the reperfusion process, subsequently suppressing the activation of Kupffer cells and neutrophils, and reducing the secretion of inflammatory factors.

Figure 5 Mn-based nanozymes for ALI alleviation.

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“…71,75 The CM NCs can scavenge and eliminate •O 2 − , H 2 O 2 , and •OH during the reperfusion process, subsequently suppressing the activation of Kupffer cells and neutrophils, and reducing the secretion of inflammatory factors.Other Transition Metal-Based NanozymesBesides Ce, Fe, Mn, and Cu-based nanozymes, other transition metal-based nanoparticles, such as zinc-based (Zn-based), nickel-based (Ni-based), molybdenum-based (Mo-based) and tungsten-based (W-based) nanoparticles, also show the enzyme catalytic activities of scavenging ROS due to their diversity of valence states and adjustability of crystal defects. https://doi.org/10.2147/IJN.S435544 DovePress International Journal of Nanomedicine 2023:18 7910…”