Mesoporous materials for encapsulating enzymes

Lee, Chia‐Hung; Lin, Tien‐Sung; Mou, Chung‐Yuan

doi:10.1016/j.nantod.2009.02.001

Cited by 434 publications

(292 citation statements)

References 119 publications

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“…Due to the large sizes, some of the module ChPs are in the surface while the others in the center of NPs. Only the module particles on the surface of NPs change their structures when nanoparticles interact with each other or with their environment [14]. The internal module particles do not directly take part in the interactions between NPs or between NPs and their environment.…”

Section: The Interactions Between Nps In the Fourth Levelmentioning

confidence: 99%

Relationship between Size and Function of Natural Substance Particles

Zhang¹

2011

Nano BioMed ENG

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All of the natural substances can be viewed as particles. According to the sizes of particles, the natural substances may be classified at six levels from small to large, and heir functional spectra can be established in the order from simple to complex. They are respectively point particles (?ppspPs), fundamental structural particles (FSPs), chemical particles (ChPs), nanoparticles (NPs), macroparticles (MPs) and celestial particles (CePs). The particles in one of these levels have their independent functional activities and interaction patterns different from those in the levels lower than In conclusion,the establishent of the size-function spectrum will be helpful to investigate the substance world as a whole, and reveal the interaction principles of the particles and biological essential fundamentals.Keywords: Size-function relations, Fundamental particles, Chemical particles, Nanoparticles, Macroparticles, Celestial particles Citation: Y. Zhang.Classification of substances based on use of nanoparticle as basical component.

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Section: The Interactions Between Nps In the Fourth Levelmentioning

confidence: 99%

Relationship between Size and Function of Natural Substance Particles

Zhang¹

2011

Nano BioMed ENG

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“…[14][15][16] Furthermore, the silanol-containing surface can be readily functionalized, [17][18][19][20][21] enabling modification with targeting molecules such as folate or hyaluronic acid to enhance cellular uptake, [22][23][24] and permitting the adsorption of various proteins with different isoelectric points (pI). 18,25 Due to their structure, MSNs protect proteins from premature degradation in body fluids, thereby increasing the efficiency of protein delivery in vivo, thus reducing renal filtration.…”

Section: Introductionmentioning

confidence: 99%

“…7,39 Proteins adsorbed at the MSNs' outer surface do not make use of the protective environment inside the MSNs, nor do they utilize the large internal surface area presented by the pores. 16,40 Thus, limitations in generating small (< 200 nm in diameter) MSNs with sufficient pore sizes (> 5 nm) to encapsulate proteins or other biomacromolecules is one of the major hurdles for "comfortably" hosting large molecules. 26,[41][42][43] In order to solve this protein inaccessibility issue, MSNs with a large pore size have been synthesized.…”

Section: Introductionmentioning

confidence: 99%

Mesoporous Silica Nanoparticles with Large Pores for the Encapsulation and Release of Proteins

Tu¹,

Boyle²,

Friedrich

et al. 2016

ACS Appl. Mater. Interfaces

120

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Mesoporous silica nanoparticles (MSNs) have been explored extensively as solid supports for proteins in biological and medical applications. Small (< 200 nm) MSNs with ordered large pores (> 5 nm), capable of encapsulating therapeutic small molecules suitable for delivery applications in vivo, are rare however. Here we present small, elongated, cuboidal, MSNs with average dimensions of 90 × 43 nm that possess disk-shaped cavities, stacked on top of each other, which run parallel to the short axis of the particle. Amine-functionalization was achieved by modifying the MSN surface with 3-aminopropyltriethoxysilane or 3-[2-(2-aminoethylamino)ethylamino] propyltrimethoxysilane (AP-MSNs and AEP-MSNs) and were shown to have similar dimensions to the non-functionalized MSNs. The dimensions of these particles, and their large surface areas as measured by nitrogen adsorption-desorption isotherms, make them ideal scaffolds for protein encapsulation and delivery. We therefore investigated the encapsulation and release behavior for seven model proteins (α-lactalbumin, ovalbumin, bovine serum albumin, catalase, hemoglobin, lysozyme and cytochrome c). It was discovered that all types of MSNs used in this study allow rapid encapsulation, with a high loading capacity, for all proteins studied. Furthermore, the release profiles of the proteins were tunable. The variation in both rate and amount of protein uptake and release was found to be determined by the surface chemistry of the MSNs, together with the isoelectric point (pI), and molecular weight of the proteins, as well as by the ionic strength of the buffer. These MSNs with their large surface area and optimal dimensions, provide a scaffold with a high encapsulation efficiency and controllable release profiles for a variety of proteins, enabling potential applications in fields such as drug delivery and protein therapy.

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“…Enzyme immobilization inside inorganic nanopores is a simple and effective way to maintain catalytic activity. [2][3][4] Surfactanttemplated mesoporous silica is a powerful candidate for an ideal host of biomolecules due to its uniform pore size of dimensions of biomolecules and tunable surface chemical properties. [4][5][6] The size-matching between the enzyme and pore size has been proposed to stabilize structure and function of the enzyme immobilized within mesoporous silica.…”

Section: Introductionmentioning

confidence: 99%

Nanoporous Waveguide Spectroscopy for the Estimation of Enzyme Adsorption on Mesoporous Silica

et al. 2017

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Mesoporous silica is considered as promising host material for enzymes due to its uniform pore size of enzyme dimensions and tunable surface chemical properties. In this study, we applied nanoporous waveguide (NPWG) spectroscopy to observe adsorption dynamics of heme proteins with different molecular size within mesoporous silica film modified with different surface functional groups. Since NPWG spectroscopy provides kinetic information and rough quantification of adsorption amount, it is useful to study the adsorption process of enzymes within inorganic nanoporous materials.

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Mesoporous materials for encapsulating enzymes

Cited by 434 publications

References 119 publications

Relationship between Size and Function of Natural Substance Particles

Relationship between Size and Function of Natural Substance Particles

Mesoporous Silica Nanoparticles with Large Pores for the Encapsulation and Release of Proteins

Nanoporous Waveguide Spectroscopy for the Estimation of Enzyme Adsorption on Mesoporous Silica

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