Mesoporous polydopamine nanoparticles carrying peptide RL-QN15 show potential for skin wound therapy

Abstract

Background Skin wound healing remains a considerable clinical challenge, thus stressing the urgent need for the development of new interventions to promote repair. Recent researches indicate that both peptides and nanoparticles may be potential therapies for the treatment of skin wounds. Methods In the current study, the mesoporous polydopamine (MPDA) nanoparticles were prepared and the peptide RL-QN15 that was previously identified from amphibian skin secretions and exhibited significant potential as a novel prohealing agent was successfully loaded onto the MPDA nanoparticles, which was confirmed by results of analysis of scanning electron microscopy and fourier transform infrared spectroscopy. The encapsulation efficiency and sustained release rate of RL-QN15 from the nanocomposites were determined. The prohealing potency of nanocomposites were evaluated by full-thickness injured wounds in both mice and swine and burn wounds in mice. Results Our results indicated that, compared with RL-QN15 alone, the prohealing potency of nanocomposites of MPDA and RL-QN15 in the full-thickness injured wounds and burn wounds in mice was increased by up to 50 times through the slow release of RL-QN15. Moreover, the load on the MPDA obviously increased the prohealing activities of RL-QN15 in full-thickness injured wounds in swine. In addition, the obvious increase in the prohealing potency of nanocomposites of MPDA and RL-QN15 was also proved by the results from histological analysis. Conclusions Based on our knowledge, this is the first research to report that the load of MPDA nanoparticles could significantly increase the prohealing potency of peptide and hence highlighted the promising potential of MPDA nanoparticles-carrying peptide RL-QN15 for skin wound therapy. Graphic abstract

Amphibian-derived peptide homodimer OA-GL17d promotes skin wound regeneration through the miR-663a/TGF-β1/Smad axis

“…To observe the effects of peptide OA-GL17d on the healing of full-thickness injury skin wounds, mice were anesthetized (1% pentobarbital sodium, 0.1 ml/20 g body weight) and two standard round cortical wounds (8 × 8 mm) were created on their dorsal skins [ 31 ]. The wounds were locally treated with 20 μl of vehicle, EGF (50 nM) or OA-GL17d (0.5, 5 and 50 nM) twice a day.…”

“…Two dorsal-skin scald wounds were created by using a 5-ml centrifuge tube (inner diameter 12 mm) with the bottom cut off and flattened. The device was pressed onto the backs of the mice and boiling water was injected into the tube with a 2-ml syringe for 20 s to form skin scald wounds [ 23 , 31 ]. From days 0 to 14, scald skin wounds were topically treated with 20 μl of vehicle, OA-GL17d (0.5, 5 and 50 nM) or EGF (50 nM).…”

Short Hexapeptide Optimized from Rice-Derived Peptide 1 Shows Promising Anti-hyperuricemia Activities

“…Histological Analysis. The removed kidney and liver tissues were fixed in 4% paraformaldehyde for 24 h. 19 Pathological changes in the kidney and liver tissue were observed via light microscopy (Zeiss, Germany) after 5 μm paraffin sectioning and hematoxylin and eosin (HE) staining for analysis of model and drug treatment of HUA in mice.…”

Mesoporous polydopamine nanoparticles for sustained release of rapamycin and reactive oxygen species scavenging to synergistically accelerate neurogenesis after spinal cord injury

“…Qin et al successfully constructed nanocomposites of mPDA and short peptide RL-QN15 for skin wound healing. 29 With the slow release of RL-QN15, the healing ability of the nanocomposites in injured or burn wounds was increased by up to 50 times compared with RL-QN15 alone.…”