Mesoporous polymeric microspheres with high affinity for phosphorylated biomolecules

Shinde, Sudhirkumar; Selvalatchmanan, Jayashree; İncel, Anıl; Akhoundian, Maedeh; Bendt, Anne K.; Torta, Federico

doi:10.1039/c8nj01114a

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…A preliminarily evaluation was carried out by comparing the extraction and elution curves of S1P from 1 mL of spiked solvent IPA/MeOH (1/1, v/v) and 1 mL of spiked human serum sample. These experiments were performed using an SPE cartridge packed with 30 mg of PD–C materials and previously optimized extraction protocols [ 28 – 30 ]. The results, depicted in Fig.…”

Section: Resultsmentioning

confidence: 99%

Enhanced selective capture of phosphomonoester lipids enabling highly sensitive detection of sphingosine 1-phosphate

Grasso,

Sommella,

Merciai

et al. 2023

Anal Bioanal Chem

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Sphingolipids play crucial roles in cellular membranes, myelin stability, and signalling responses to physiological cues and stress. Among them, sphingosine 1-phosphate (S1P) has been recognized as a relevant biomarker for neurodegenerative diseases, and its analogue FTY-720 has been approved by the FDA for the treatment of relapsing–remitting multiple sclerosis. Focusing on these targets, we here report three novel polymeric capture phases for the selective extraction of the natural biomarker and its analogue drug. To enhance analytical performance, we employed different synthetic approaches using a cationic monomer and a hydrophobic copolymer of styrene-DVB. Results have demonstrated high affinity of the sorbents towards S1P and fingolimod phosphate (FTY-720-P, FP). This evidence proved that lipids containing phosphate diester moiety in their structures did not constitute obstacles for the interaction of phosphate monoester lipids when loaded into an SPE cartridge. Our suggested approach offers a valuable tool for developing efficient analytical procedures. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Enhanced selective capture of phosphomonoester lipids enabling highly sensitive detection of sphingosine 1-phosphate

Grasso,

Sommella,

Merciai

et al. 2023

Anal Bioanal Chem

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“…The development of abiotic phosphate receptors is crucial for alternative robust, reproducible, and low-cost binders as efficient tools to target and detect biologically, chemically, and environmentally important phosphates. , Even though much effort has been devoted, the synthesis of specific binders for oxyanions particularly targeting phosphorylated and sulfated compounds is still demanding in the field of supramolecular chemistry. ,− To this end, highly selective and refined synthetic receptors capable of recognition of oxyanion guests have been developed based on neutral host functional monomers by the imprinting approach. ,,,,, Functional monomers are allowed to interact with a template followed by polymerization in the presence of a cross-linking agent. Subsequent removal of the template from the cross-linked polymer matrix leaves behind three-dimensional binding sites that are complementary in size, shape, and functionality, which allows an affinity for the template or a template substructure containing larger molecules.…”

Section: Introductionmentioning

confidence: 99%

Role of Comonomers in the Recognition of Anionic Biomolecules in Water: Hydrogen-Bonded Imprinted Polymeric Receptor

Rouhi

İncel

Shinde

2021

ACS Appl. Polym. Mater.

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The field of anion recognition in aqueous media is dominated by multiple positive charges and/or metal ions containing synthetic receptors. Here, we report the synthesis of a highly water-compatible oxyanion-imprinted neutral receptor by cross-linking the polymerizable diaryl urea host monomer and phenyl phosphonic acid (PPA) complex in the presence of a large number of comonomers containing hydroxyl or methyl ester groups. We have demonstrated that the affinity of the imprinted receptor for anions is easily tuned by decorating the hydrophilic and hydrophobic end groups around the binding pocket of the imprinted receptor. The binding characteristics of the synthesized artificial binders were evaluated in aqueous and nonaqueous environments. We have demonstrated practical applicability of the PPA-imprinted receptor by packing in the solid-phase extraction cartridge which was used to separate the ubiquitous pesticide glyphosate from water. Also, the developed receptor can selectively bind to phosphorylated tyrosine amino acids and phosphorylated tyrosine octapeptides in aqueous buffered media than to other phosphorylated and nonphosphorylated analogues. The approach utilized here to use an inexpensive dummy template can be utilized for the synthesis of imprinted receptors for anions with phosphate motifs.

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TiO2-modified fibrous core-shell mesoporous material to selectively enrich endogenous phosphopeptides with proteins exclusion prior to CE-MS analysis

Azhar

Feng

Liu

et al. 2021

Talanta

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Mesoporous polymeric microspheres with high affinity for phosphorylated biomolecules

Abstract: Bis-imidazolium functionalized mesoporous microspheres selectively extract phosphorylated peptides/lipids from biofluids.

Cited by 3 publications

References 23 publications

Enhanced selective capture of phosphomonoester lipids enabling highly sensitive detection of sphingosine 1-phosphate

Enhanced selective capture of phosphomonoester lipids enabling highly sensitive detection of sphingosine 1-phosphate

Role of Comonomers in the Recognition of Anionic Biomolecules in Water: Hydrogen-Bonded Imprinted Polymeric Receptor

TiO2-modified fibrous core-shell mesoporous material to selectively enrich endogenous phosphopeptides with proteins exclusion prior to CE-MS analysis

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