Mesorhizobium delmotii and Mesorhizobium prunaredense are two new species containing rhizobial strains within the symbiovar anthyllidis

Mohamad, Roba; Willems, Anne; Quéré, Antoine Le; Maynaud, Géraldine; Pervent, Marjorie; Bonabaud, Maurine; Dubois, Emeric; Cleyet-Marel, Jean-Claude; Brunel, Brigitte

doi:10.1016/j.syapm.2017.01.004

Cited by 30 publications

(12 citation statements)

References 42 publications

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“…Both type strains were first isolated from the root nodules of the legume plant Anthyllis vulneraria subsp. carpatica, grown on soils sampled at the border of the Avinières mine and at a non-mining site in France (Mohamad et al, 2017). It was shown that the strains M. delmotii STM4623 T and M. prunaredense STM4891 T were able to form nodules…”

Section: Resultsmentioning

confidence: 99%

“…and exhibit nitrogen-fixing activity with their original host plant Anthyllis vulneraria, which is widespread in Europe and is resistant to heavy metals (Mohamad et al, 2017). Cluster II was formed by isolates Gu1/B2-3, Gu5/KM2-3 and type strains M. delmotii STM4623 T and M. prunaredense STM4891 T , with which these isolates showed a 100 % level of rrs-similarity (Table 2; Fig.…”

Section: Symbio-geneticsmentioning

confidence: 99%

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Genetic diversity of rhizobial strains isolated from the relict legumes Gueldenstaedtia monophylla and G. verna growing in the republics of Altai and Buryatia (Russia)

Karlov

Sazanova

Guro

et al. 2022

BioComm

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For the first time, bacteria were isolated and identified from the root nodules of relict legumes Gueldenstaedtia monophylla Fisch. and G. verna (Georgi) Boriss. growing in the republics of Altai and Buryatia. The taxonomic position of the 29 obtained isolates was determined by sequencing the 16S rRNA gene (rrs). Showing a significant biodiversity, the isolates from G. monophylla and G. verna belonged to five genera of the order Rhizobiales: Mesorhizobium and Phyllobacterium (family Phyllobacteriaceae), Rhizobium (family Rhizobiaceae), Bosea (family Boseaceae), Bradyrhizobium (family Bradyrhizobiaceae). Three isolates which belonged to the species Bradyrhizobium valentinum and Rhizobium alamii showed 100 % of rrs-similarity with the type strains B. valentinum LmjM3T and R. alamii GBV016T, respectively. Six isolates of the genera Bosea and Rhizobium had a low level of rrs-similarity with the closest type strains (less than 99.5 %), which indicates that they may be assigned to new species. The data obtained can be used to itemise taxonomy within the order Rhizobiales, as well as to reveal the mechanisms of the formation of specific plant-microbial relationships during the evolution of symbiosis by studying the intermediate link between the extinct and modern rhizobia-legume symbiotic systems.

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Section: Resultsmentioning

confidence: 99%

Section: Symbio-geneticsmentioning

confidence: 99%

Genetic diversity of rhizobial strains isolated from the relict legumes Gueldenstaedtia monophylla and G. verna growing in the republics of Altai and Buryatia (Russia)

Karlov

Sazanova

Guro

et al. 2022

BioComm

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“…vulneraria isolated from moderately and uncontaminated sites, respectively [32]. The study from Mohamad and colleagues [31] clearly suggested a greater diversity among Anthyllis mesorhizobia in uncontaminated soils.…”

Section: Introductionmentioning

confidence: 95%

“…vulneraria (Aminobacter anthyllidis [27]). Phylogenetic trees were inferred as described previously [32]. Briefly, phylogenetic trees were reconstructed by maximum-likelihood (ML).…”

Section: Dna Extraction Sequencing and Phylogenetic Analysesmentioning

confidence: 99%

Mesorhizobium ventifaucium sp. nov. and Mesorhizobium escarrei sp. nov., two novel root-nodulating species isolated from Anthyllis vulneraria

Mohamad

Willems

Quéré

et al. 2022

Systematic and Applied Microbiology

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“…2008; Mohamad et al . 2017) reported within genus Mesorhizobium . For this putative new symbiovar, the name ‘aridi’ is proposed.…”

Section: Introductionmentioning

confidence: 99%

Identification of the endosymbionts from Sulla spinosissima growing in a lead mine tailings in Eastern Morocco as Mesorhizobium camelthorni sv. aridi

Lamin

Alami

Bouhnik

et al. 2020

J. Appl. Microbiol.

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Aims To identify the bacteria nodulating Sulla spinosissima growing profusely in a lead and zinc mine tailings in Eastern Morocco. Methods and Results In all, 32 rhizobial cultures, isolated from root nodules of S. spinosissima growing in soils of the mining site, were tolerant to different heavy metals. The ERIC‐polymerase chain reaction (PCR) fingerprinting analysis clustered the isolates into seven different groups, and the analysis of the 16S rRNA sequences of four selected representative strains, showed they were related to different species of the genus Mesorhizobium. The atpD, glnII and recA housekeeping genes analysis confirmed the affiliation of the four representative strains to Mesorhizobium camelthorni CCNWXJ40‐4T, with similarity percentages varying from 96·30 to 98·30%. The sequences of the nifH gene had 97·33–97·78% similarities with that of M. camelthorni CCNWXJ40‐4T; however, the nodC phylogeny of the four strains diverged from the type and other reference strains of M. camelthorni and formed a separated cluster. The four strains nodulate also Astragalus gombiformis and A. armatus but did not nodulate A. boeticus, Vachellia gummifera, Prosopis chilensis, Cicer arietinum, Lens culinaris, Medicago truncatula, Lupinus luteus or Phaseolus vulgaris. Conclusions Based on similarities of the nodC symbiotic gene and differences in the host range, the strains isolated from S. spinosissima growing in soils of the Sidi Boubker mining site may form a different symbiovar within Mesorhizobium for which the name aridi is proposed. Significance and Impact of the Study In this work, we show that strains of M. camelthorni species nodulating S. spinosissima in the arid area of Eastern Morocco constitute a distinct phylogenetic clade of nodulation genes; we named symbiovar aridi, which encompasses also mesorhizobia from other Mediterranean desert legumes.

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Mesorhizobium delmotii and Mesorhizobium prunaredense are two new species containing rhizobial strains within the symbiovar anthyllidis

Cited by 30 publications

References 42 publications

Genetic diversity of rhizobial strains isolated from the relict legumes <em>Gueldenstaedtia monophylla</em> and <em>G. verna</em> growing in the republics of Altai and Buryatia (Russia)

Genetic diversity of rhizobial strains isolated from the relict legumes <em>Gueldenstaedtia monophylla</em> and <em>G. verna</em> growing in the republics of Altai and Buryatia (Russia)

Mesorhizobium ventifaucium sp. nov. and Mesorhizobium escarrei sp. nov., two novel root-nodulating species isolated from Anthyllis vulneraria

Identification of the endosymbionts from Sulla spinosissima growing in a lead mine tailings in Eastern Morocco as Mesorhizobium camelthorni sv. aridi

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