Mesos components (CaCl2, MgSO4, KH2PO4) are critical for improving pear micropropagation

Wada, Sugae; Niedz, Randall P.; DeNoma, Jeanine; Reed, Barbara M.

doi:10.1007/s11627-013-9508-x

Cited by 37 publications

(21 citation statements)

References 21 publications

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“…Adjustment of mineral elements in the culture medium for micropropagation was found beneficial in "three 'Vriesea bromeliads' (Aranda-Peres and Martinelli 2009) and Picconia azorica (Mendonça et al 2015). Similar results were also observed in hazelnuts (Nas and Read 2004), Dalbergia sissoo (Vibha et al 2014) and Pyrus species (Wada et al 2013). The cultures were maintained (Fig.…”

Section: Effect Of Pgrs and Modified Media On Shoot Multiplicationmentioning

confidence: 55%

“…Similarly, potassium is required for protein synthesis and to maintain turgor pressure of the cell. Hence Mg and K play a predominant role in plant growth during shoot development (Ramage and Williams 2002;Hermans et al 2004;Wada et al 2013). Further, ammonium sulphate is suggested to promote the nitrate absorption and buffers the nutrient medium, thus improve the overall growth/health of cultures (Lodha et al 2014a).…”

Section: Effect Of Pgrs and Modified Media On Shoot Multiplicationmentioning

confidence: 99%

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A high-frequency in vitro multiplication, micromorphological studies and ex vitro rooting of Cadaba fruticosa (L.) Druce (Bahuguni): a multipurpose endangered medicinal shrub

Lodha

Patel

Shekhawat

2015

Physiol Mol Biol Plants

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An efficient and reproducible in vitro propagation protocol has been established for Cadaba fruticosa (L.) Druce. Surface-sterilized nodal stem segments of mature plant were used as explants for culture establishment. Multiple shoots were optimally differentiated from the nodal stem explants through bud breaking on Murashige and Skoog (1962) medium containing 3.0 mg l(-1) benzyladenine (BA). The effect of different plant growth regulators and minerals were studied on different stages of micropropagation procedure (i.e., explant establishment, shoot multiplication/growth and ex vitro rooting). Additionally, for enhancing shoot multiplication during subculture, MS medium was modified (MMS) with higher levels of magnesium, potassium and sulphate ions. Out of these, MMS3 medium containing 0.25 mg l(-1) each of BA and Kin (N6-furfuryladenine), with 0.1 mg l(-1) NAA (α-naphthalene acetic acid) was found the best for shoot multiplication (42.45 ± 3.82 per culture vessel). The in vitro regenerated shoots were rooted under ex vitro conditions on treating the shoot base with 500 mg l(-1) of IBA (indole-3 butyric acid) for 3 min on sterile Soilrite®. The ex vitro rooted plants were hardened in the greenhouse and transferred to the field with ≈85 % survival rate. There were not any visual differences between wild and micropropagated plants in the field, although the later underwent significant changes during acclimatization. Micromorphological changes on leaf surface characters from in vitro to acclimatized plantlets were studied in terms of development of glandular trichomes, changes in vein spacing and vein structure in order to understand the nature of plant responses towards environmental conditions. The method developed and defined can be applied for commercial cultivation, which may be important for extraction of bioactive compounds and may facilitate conservation of this multipurpose endangered medicinal shrub.

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Section: Effect Of Pgrs and Modified Media On Shoot Multiplicationmentioning

confidence: 55%

Section: Effect Of Pgrs and Modified Media On Shoot Multiplicationmentioning

confidence: 99%

A high-frequency in vitro multiplication, micromorphological studies and ex vitro rooting of Cadaba fruticosa (L.) Druce (Bahuguni): a multipurpose endangered medicinal shrub

Lodha

Patel

Shekhawat

2015

Physiol Mol Biol Plants

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“…There is much statistical software for optimizing the growth medium for in vitro plant culture (Gago et al, 2010; Gallego et al, 2011). Response surface method (RSM) is one of the software that has been repeatedly used to optimize growth medium for in vitro culture of pear genotypes (Reed et al, 2013a,b; Wada et al, 2013, 2015). Previous studies detected that ANN-GA models had a substantially higher accuracy of prediction than RSM models (Sedghi et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Predicting In vitro Culture Medium Macro-Nutrients Composition for Pear Rootstocks Using Regression Analysis and Neural Network Models

et al. 2016

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Two modeling techniques [artificial neural network-genetic algorithm (ANN-GA) and stepwise regression analysis] were used to predict the effect of medium macro-nutrients on in vitro performance of pear rootstocks (OHF and Pyrodwarf). The ANN-GA described associations between investigating eight macronutrients (NO3-, NH4+, Ca2+, K+, Mg2+, PO42-, SO42-, and Cl−) and explant growth parameters [proliferation rate (PR), shoot length (SL), shoot tip necrosis (STN), chlorosis (Chl), and vitrification (Vitri)]. ANN-GA revealed a substantially higher accuracy of prediction than for regression models. According to the ANN-GA results, among the input variables concentrations (mM), NH4+ (301.7), and NO3-, NH4+ (64), SO42- (54.1), K+ (40.4), and NO3- (35.1) in OHF and Ca2+ (23.7), NH4+ (10.7), NO3- (9.1), NH4+ (317.6), and NH4+ (79.6) in Pyrodwarf had the highest values of VSR in data set, respectively, for PR, SL, STN, Chl, and Vitri. The ANN-GA showed that media containing (mM) 62.5 NO3-, 5.7 NH4+, 2.7 Ca2+, 31.5 K+, 3.3 Mg2+, 2.6 PO42-, 5.6 SO42-, and 3.5 Cl− could lead to optimal PR for OHF and optimal PR for Pyrodwarf may be obtained with media containing 25.6 NO3-, 13.1 NH4+, 5.5 Ca2+, 35.7 K+, 1.5 Mg2+, 2.1 PO42-, 3.6 SO42-, and 3 Cl−.

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“…“Pasadena” (Niedz et al, 2014); CaCl 2 .2H 2 O, MgSO 4 .7H 2 O and KH 2 PO 4 in Pyrus sp . (Wada et al, 2013) and Rubus idaeus L. (Poothong and Reed, 2014, 2015); KH 2 PO 4 , K 2 SO 4 and NH 4 NO 3 in Corylus avellana L. (Akin et al, 2017b) ; KH 2 PO 4 and MgSO 4 .7H 2 O in Prunus armeniaca Lam (Kovalchuk et al, 2017) in vitro culture have been reported. The use of salts as factors, in all of these studies, implies a problem of ion confounding, being difficult to identify exactly corresponding ion(s) impacting the parameter (Niedz and Evens, 2006).…”

Section: Discussionmentioning

confidence: 98%

Combining DOE With Neurofuzzy Logic for Healthy Mineral Nutrition of Pistachio Rootstocks in vitro Culture

et al. 2018

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The aim of this study was to determine the effects of Murashige and Skoog (MS) salts on optimal growth of two pistachio rootstocks, P. vera cv. “Ghazvini” and “UCB1” using design of experiments (DOE) and artificial intelligence (AI) tools. MS medium with 14 macro—and micro-elements was used as base point and its concentration varied from 0 to 5 × MS concentrations. Design of experiments (DOE) software was used to generate a five-dimensional design space by categorizing MS salts into five independent factors (NH4NO3, KNO3, mesos, micros and iron), reducing the experimental design space from 3,125 to just 29 treatments. Typical plant growth parameters such as shoot quality (SQ), proliferation rate (PR), shoot length (SL), and some physiological disorders including shoot-tip necrosis (STN) and callus formation at the base of explants (BC) were evaluated for each treatment. The results were successfully modeled using neurofuzzy logic software. The model delivered new insights, by different sets of “IF–THEN” rules, pinpointing the key role of some ion interactions (SO42- × Cl−, K+ × SO42- × EDTA−, and Fe2+ × Cu2+ × NO3-) for SQ, PR, and SL, whilst physiological disorders (STN and BC) were governed mainly by independent ions as Fe2+ and EDTA−, respectively. In our opinion, the methodology and results obtained in this study is extremely useful to understand the effect of mineral nutrients on pistachio in vitro culture, through discovering new complex interactions among macro—and micro-elements which can be implemented to design new media of plant tissue culture and improve healthy plant micropropagation for any plant species.

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Mesos components (CaCl2, MgSO4, KH2PO4) are critical for improving pear micropropagation

Cited by 37 publications

References 21 publications

A high-frequency in vitro multiplication, micromorphological studies and ex vitro rooting of Cadaba fruticosa (L.) Druce (Bahuguni): a multipurpose endangered medicinal shrub

A high-frequency in vitro multiplication, micromorphological studies and ex vitro rooting of Cadaba fruticosa (L.) Druce (Bahuguni): a multipurpose endangered medicinal shrub

Predicting In vitro Culture Medium Macro-Nutrients Composition for Pear Rootstocks Using Regression Analysis and Neural Network Models

Combining DOE With Neurofuzzy Logic for Healthy Mineral Nutrition of Pistachio Rootstocks in vitro Culture

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