Mesotheliomas in rats following inoculation with acid-leached chrysotile asbestos and other mineral fibres

Monchaux, G.; Bignon, J.; Jaurand, Marie‐Claude; Lafuma, J.; Sébastien, P.; Massé, R.; Hirsch, A; Goni, J.

doi:10.1093/carcin/2.3.229

Cited by 102 publications

(38 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Leaching another sample of the same fibers with HCI for 2 hr reduced their carcinogenic potency, while NaOH treatment for 24 hr did not appear to alter their carcinogenicity (3,4). The reduction of carcinogenicity of acid-leached chrysotile in intrapleural experiments had been observed previously (5,6). However, the authors did not explain the effect by a reduced durability but rather by altered surface properties of the fibers and the loss of carcinogenic components by leaching.…”

Section: Introduction -Early Resultsmentioning

confidence: 60%

Significance of Durability of Mineral Fibers for Their Toxicity and Carcinogenic Potency in the Abdominal Cavity of Rats in Comparison with the Low Sensitivity of Inhalation Studies

Pott¹,

Roller²,

Kamino³

et al. 1994

Environmental Health Perspectives

View full text Add to dashboard Cite

At the same time that carcinogenicity of very thin glass fibers after intrapleural and intraperitoneal (ip) administration was demonstrated (1,2) researchers found that gypsum fibers and HCI-leached chrysotile fibers were easily soluble in the peritoneal cavity. This led to the conclusion that the chemical composition of fibers was not responsible for the carcinogenesis but that the degree of carcinogenic potency of a fiber depended on the extent to which it retained its fibrous structure. A thin glass fiber with a low biodurability did not induce tumors after ip injection of a high dose, although the ip test had been criticized for being "overly sensitive." The ip model has been the most successful for determining carcinogenicity of inorganic fibers and establishing dose-response relationships; but to determine the possibilities and limitations of this test model, very high doses of nonfibrous silicon carbide and of a slightly durable glass fiber type were injected ip in Wistar rats. No obviously acute or chronic toxic effect was observed in 90 weeks, but there was a 40% incidence of serosal tumors in the group treated with glass fibers. A pilot study on the persistence of slag fibers in the omentum of rats after ip injection showed a half-time of about 1 year. It was calculated that an ip injection of 109 fibers would lead to a concentration of fiber numbers in the ash of the omentum in the same range as the concentration in the lung after 2 years of inhalation exposure. The long-term inhalation study with fibers in rats has been called the "gold standard" for risk characterization. However, the tumor risk from inhalation of asbestos fibers in man has been estimated to be about 200 times higher per fiber than the risk in rats. The concentration of crocidolite fibers in the lung of rats in an inhalation study that was negative was more than 1 000-fold higher than the median concentration of amphibole fibers in the lungs of asbestos workers with mesotheliomas. This great difference clearly indicates that the sensitivity of the inhalation model is too low for the identification of the carcinogenic potential of mineral fibers. -Environ Health Perspect 102(Suppl. 5): 145-150 (19941

show abstract

Section: Introduction -Early Resultsmentioning

confidence: 60%

Significance of Durability of Mineral Fibers for Their Toxicity and Carcinogenic Potency in the Abdominal Cavity of Rats in Comparison with the Low Sensitivity of Inhalation Studies

Pott¹,

Roller²,

Kamino³

et al. 1994

Environmental Health Perspectives

View full text Add to dashboard Cite

show abstract

“…inoculation of a mixture composed of 5 mg kg -1 xylazine (ND Rompun 2%) (Bayer Pharma, Puteaux, France) and 40 mg kg -1 of Ketamin (ND Imalgene, Rhône Mérieux, Lyon, France). The method used to permit orthotopic growth of mesothelioma cells consisted in the deposition in the pleural cavity of a cell suspension derived from the procedure developed to inject asbestos fibres in the pleural cavity (Monchaux et al, 1981). The desired amount of mesothelioma cells was suspended in 0.3 ml of phosphatebuffered saline (PBS) and sucked into a 1 ml syringe, then fitted to a 0.8 mm diameter needle.…”

Section: Cell Transplantation Methodsmentioning

confidence: 99%

Pleuro-pulmonary tumours detected by clinical and chest X-ray analyses in rats transplanted with mesothelioma cells

et al. 1999

View full text Add to dashboard Cite

Summary New strategies for cancer therapy must be developed, especially in severe neoplasms such as malignant pleural mesothelioma. Animal models of cancer, as close as possible to the human situation, are needed to investigate novel therapeutical approaches. Orthotopic transplantation of cancer cells is then relevant and efforts should be made to follow up tumour evolution in animals. In the present study, we developed a method for the orthotopic growth of mesothelioma cells in the pleural cavity of Fischer 344 and nude rats, along with a procedure for clinical survey. Two mesothelioma cell lines, of rat and human origin, were inoculated by transthoracic puncture. Body weight determination and chest X-ray analyses permitted the follow-up of tumour evolution by identifying different stages. Autopsies showed that tumours localized on the whole pleural cavity (diaphragm, parietal pleura), mediastinum and pericardium. Tumour morphology and antigenic characteristics were consistent with those of the inoculated cells and were similar in both types of rats inoculated with the same cell type. These results demonstrate that mesothelioma formation in rats can be followed up by clinical and radiographic survey after gentle intrathoracic inoculation of mesothelioma cells, thus allowing the definition of stages of interest for further experimental trials.

show abstract

“…Magnesium is leached from the surface of the chrysotile fiber, apparently causing fragmentation and its faster disappearance from the lung in comparison to amphiboles. If the magnesium is leached before the chrysotile is administered, the ability of the chrysotile to cause mesothe lioma in animals is reduced, although the leached material also contained shorter, thicker fibers than the unleached material, in one report (40).…”

Section: Durjbilitymentioning

confidence: 99%

Airborne Asbestos and Public Health

Davis¹,

Mandula²

1985

Annu. Rev. Public Health

View full text Add to dashboard Cite

Mesotheliomas in rats following inoculation with acid-leached chrysotile asbestos and other mineral fibres

Cited by 102 publications

References 0 publications

Significance of Durability of Mineral Fibers for Their Toxicity and Carcinogenic Potency in the Abdominal Cavity of Rats in Comparison with the Low Sensitivity of Inhalation Studies

Significance of Durability of Mineral Fibers for Their Toxicity and Carcinogenic Potency in the Abdominal Cavity of Rats in Comparison with the Low Sensitivity of Inhalation Studies

Pleuro-pulmonary tumours detected by clinical and chest X-ray analyses in rats transplanted with mesothelioma cells

Airborne Asbestos and Public Health

Contact Info

Product

Resources

About