Messenger RNA Expression and Genetic Polymorphisms of Cell Cycle Control Genes and Chromosomal Aberrations in Chinese Vinyl Chloride Monomer-Exposed Workers

Qiu, Yulan; Sun, Pin; Wang, Wei; Wang, Tong; Zhang, Xia

doi:10.1097/jom.0b013e3182363bfb

Cited by 6 publications

(6 citation statements)

References 14 publications

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“…Studies have shown that the 40 bp missing genotype (SS) for the mdm2 rs3730485 locus may be a risk factor for uterine fibroids [ 36 ]; other studies also suggested that the SS type of this locus was a risk factor for hepatocellular carcinoma [ 37 ]. Although there was no report regarding the relationship between p53 genetic polymorphisms and ChA in workers exposed to organophosphate pesticides, some researchers have studied the relationship between the three genetic polymorphism loci and micronucleus rate, and found no association between the three polymorphisms and micronucleus rate [ 38 ]. Again, we were also concerned regarding the potential biological mechanism under this interaction model among these SNPs and environmental factors in addition to the statistical significance.…”

Section: Discussionmentioning

confidence: 99%

Interaction between polymorphisms in cell-cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoate

et al. 2018

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Cholinesterase activity (ChA), the effective biomarker for organophosphate pesticide exposure, is possibly affected by single nucleotide polymorphisms (SNPs) in cell-cycle-related genes. One hundred and eighty workers with long-term exposure to omethoate and 115 healthy controls were recruited to explore the gene–gene and gene–environment interactions. The acetylthiocholine and dithio-bis-(nitrobenzoic acid) method was used to detect the cholinesterase activities in whole blood, erythrocytes and plasma. Genetic polymorphisms were determined by the PCR-RFLP and direct PCR electrophoresis methods. Statistical results showed that the cholinesterase activities of whole blood, erythrocytes and plasma in the exposure group were significantly lower than those in the control group (p < 0.001), and erythrocyte cholinesterase activities were associated with gender, smoking and drinking in the exposure group (p < 0.05). Single-locus analyses showed that there is a statistically significant difference in the ChA among the genotypes CC, CA and AA of the p21 rs1801270 locus in the control group (p = 0.033), but not in the exposure group. A significant interaction between genes and environmental factors (i.e. p53, p21, mdm2, gender, smoking and drinking) affecting ChA was found through a generalized multifactor dimensionality reduction analysis. These obtained markers will be useful in further marker-assisted selection in workers with exposure to omethoate.

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Section: Discussionmentioning

confidence: 99%

Interaction between polymorphisms in cell-cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoate

et al. 2018

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“…Among them, the deletion in p53 rs17878362 and MDM2 rs3730485 can be detected by PCR electrophoresis directly; the PCRrestriction fragment length polymorphism (PCR-RFLP) method detected other loci for genotyping, primer sequences, and restriction endonucleases as described previously. [23][24][25][26][27] The PCR was carried out in a volume of 15 μL containing 50 ng of DNA, 7.5 μL of 2× PCR master Mix (Shanghai Lifefeng Biotech Co., Ltd), and 3 pmol of each primer. PCR reaction conditions were as follows: first, incubated at 94°C for 5 min, then entered into the cycling program of denaturation-annealing-extension to select a different annealing temperature depending on the Tm value and pre-experimental results, followed by a final extension at 72°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…Among them, the deletion in p53 rs17878362 and MDM2 rs3730485 can be detected by PCR electrophoresis directly; the PCR–restriction fragment length polymorphism (PCR-RFLP) method detected other loci for genotyping, primer sequences, and restriction endonucleases as described previously. 23–27…”

Section: Methodsmentioning

confidence: 99%

Changes in the expression of genes involved in cell cycle regulation and the relative telomere length in the process of canceration induced by omethoate

et al. 2017

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Organophosphorous pesticides (OPs), with high efficiency, broad-spectrum and low residue, are widely used in China. Omethoate is a broad category of organophosphorous pesticides and is more domestically utilized which has chronic toxic effect on human health caused by long-term, low-dose exposure to Ops, recently its potential genotoxicity has attracted wide attention which can cause chromosomal DNA damage. Thus, the aim of this study is screen susceptible biomarkers and explore the mechanism of canceration induced by omethoate. 180 long-term organophosphorus pesticide-exposed workers and 115 healthy controls were recruited. Quantitative polymerase chain reaction method was applied to determine the relative telomere length in peripheral lymphocyte DNA as well as p53 and p21 gene expression levels. Genetic polymorphisms were determined by the polymerase chain reaction-restriction fragment length polymorphism method. Multiple linear regression was conducted to explore the effects of exposure, expression levels, and polymorphisms in genes on the telomere length. The results showed the relative telomere lengths in the exposure group were significantly longer than that in the control group. The messenger RNA expression levels of p53 and p21 in exposure group were significantly lower than that in the control group; telomere lengths of the CA genotype individuals of p21 rs1801270 polymorphism locus were significantly longer than that of the CC genotype in the control group that were estimated using the Bonferroni method; and bivariate correlation analysis showed that the messenger RNA expression level of gene p53 was negatively correlated with telomere length, and the messenger RNA expression level of gene p21 was positively correlated with telomere length. Multivariate analysis found that p53 messenger RNA and p21 messenger RNA had an impact on telomere length. These results demonstrated that the messenger RNA expression levels of p53 and p21 may have a relationship with the changes in telomere length induced by omethoate and provided strong evidence for the mechanism of canceration induced by poison.

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“…The quantities of mRNA expression of p53 and p21 genes were detected by real-time polymerase chain reaction and the authors found an increased mRNA expression of p53 and a decreased expression of p21 [95].…”

Section: Transcriptomicsmentioning

confidence: 99%

Molecular Fingerprints of Environmental Carcinogens in Human Cancer

Ceccaroli

Pulliero

Geretto

et al. 2015

Journal of Environmental Science and Health, Part C

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Identification of specific molecular changes (fingerprints) is important to identify cancer etiology. Exploitable biomarkers are related to DNA, epigenetics, and proteins. DNA adducts are the turning point between environmental exposures and biological damage. DNA mutational fingerprints are induced by carcinogens in tumor suppressor and oncogenes. In an epigenetic domain, methylation changes occurs in specific genes for arsenic, benzene, chromium, and cigarette smoke. Alteration of specific microRNA has been reported for environmental carcinogens. Benzo(a)pyrene, cadmium, coal, and wood dust hits specific heat-shock proteins and metalloproteases. The multiple analysis of these biomarkers provides information on the carcinogenic mechanisms activated by exposure to environmental carcinogens.

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Messenger RNA Expression and Genetic Polymorphisms of Cell Cycle Control Genes and Chromosomal Aberrations in Chinese Vinyl Chloride Monomer-Exposed Workers

Abstract: Messenger RNA expressions of p53 and p21 are changed with VCM-exposure status.

Cited by 6 publications

References 14 publications

Interaction between polymorphisms in cell-cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoate

Interaction between polymorphisms in cell-cycle genes and environmental factors in regulating cholinesterase activity in people with exposure to omethoate

Changes in the expression of genes involved in cell cycle regulation and the relative telomere length in the process of canceration induced by omethoate

Molecular Fingerprints of Environmental Carcinogens in Human Cancer

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