Messenger RNA Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Human Hepatocellular Carcinoma

Abstract

Our results suggest that semiquantitative RT-PCR analysis of MMPs may be helpful in disease management of patients with hepatocellular carcinoma.

The expression of matrix metalloproteinase matrilysin indicates the degree of inflammation in ulcerative colitis

“…23,29 It has been reported that an imbalance of MMPs and TIMPs can induce or exacerbate several diseases. 8,9 We noted that, in the bowel of UC patients, the overexpression of MMPs, mainly matrilysin, and the hypoexpression of TIMPs were observed. These observations are consistent with previous reports.…”

“…These observations are consistent with previous reports. 8 It is noteworthy that the reduced expression of TIMPs compared with the expression of matrilysin, is related to severe inflammation grades. The use of matrilysin as a molecular marker may become a routine part of the management of patients with UC, thus helping to define appropriate therapeutic and follow-up protocols.…”

“…The balance between MMPs and TIMPs, which is critical in maintaining the integrity of the ECM, is often shifted in favor of the proteases during pathological conditions and results in an excessive proteolytic degradation of the ECM. 8,9 The MMPs are involved in tissue remodeling, angiogenesis, and the promotion of leucocyte extravasation in an actively inflamed area of the ulcer base in UC. Several studies have reported the expression of MMPs in intestinal ulcerations, including interstitial collagenase (MMP-1), gelatinase-A (MMP-2), stromelysin-1 (MMP-3), matrilysin (MMP-7), neutrophil collagenase (MMP-8), gelatinase-B (MMP-9), stromelysin-2 (MMP-10), macrophage metalloelastase (MMP-12), Background.…”

Clinical Relevance of the Expression of mRNA of MMP-7, MMP-9, TIMP-1, TIMP-2 and CEA Tissue Samples from Colorectal Liver Metastases

“…Waas et al [17] concluded that the preoperative plasma proMMP-2, proMMP-9 and TIMP-1 levels have no potential as diagnostic or prognostic marker in CLM. In 1999, Yamamoto et al [26] reported that increased mRNA expression of TIMP-1 and TIMP-2 was observed in tumor tissue in half of the patients with hepatocellular carcinoma, but did not correlate with any clinicopathological features using semiquantitative RT-PCR for mRNA determination. We used quantitative RT-PCR, and found that the mRNA level of TIMP-1 expression was statistically significantly associated with DFI and OS.…”

Matrix metalloproteinases in cancer: Prognostic markers and therapeutic targets

“…Similarly, expression of activated MMP-2 is related to regional lymph node and distal metastasis as well as to postresection recurrence of the same tumour. 56 Concomitant overexpression of MMP-2 and MMP-7 is associated with recurrence in hepatocellular cancer, 57 and elevated MMP-9 expression in tumor cells is related to recurrence in superficial carcinoma of the urinary bladder. 58 The expression of certain MMPs in primary tumour can predict the risk of metastasis.…”