Messenger RNAs from the transforming region of bovine papilloma virus type I

Stenlund, Arne; Zabielski, J.; Ahola, Harri; Moreno‐López, J.; Pettersson, Ulf

doi:10.1016/0022-2836(85)90240-2

Cited by 119 publications

(119 citation statements)

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“…Although no TATA homologies or any other regulatory elements were found upstream of the 5' termini mapped above, minor viral transcripts mapping to analogous genomic positions have been found in BPV-l-induced fibropapillomas (Baker & HoMey, 1987) and in BPV-1-transformed mouse C127 fibroblasts (Stenlund et al, 1985). Multiple initiation sites were mapped around nt 2440 and nt 3080 (Ahola et al, 1987;Baker & HoMey, 1987), the latter giving rise to transcripts potentially encoding the E2 transcriptional repressor protein (Lambert et aL, 1987).…”

Section: Protein-coding Potential Of the Minor Transcriptsmentioning

confidence: 94%

“…However, transcriptional analyses have revealed a complex 0000-8456 © 1988 SGM mRNA splicing pattern in papillomaviruses which frequently joins parts of different ORFs to create new genes (Stenlund et al, 1985;Baker & Howley, 1987;Chow et al, 1987a, b), the functions of which may differ from those of the individual ORFs assayed in vitro. In such cases, functional dissection may be more realistically undertaken by the expression of cDNAs in tissue culture assay systems.…”

Section: Introductionmentioning

confidence: 99%

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Mapping of Two Novel Transcripts of Bovine Papillomavirus Type 4

1988

Journal of General Virology

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SUMMARYA cDNA library was synthesized using RNA from bovine papillomavirus type 4 (BPV-4)-induced papillomas. The major viral transcript was characterized by sequencing of its cDNA, primer extension mapping and S 1 nuclease protection studies. The transcript initiates at multiple sites between nucleotide (nt) 777 and nt 902, contains a splice junction between nt 1016 and nt 3376 and terminates at nt 4034, using the early polyadenylation signal at nt 4009. Sequencing of viral cDNAs and mRNA revealed deviations from the reported genomic sequence between nt 3412 and nt 3460. These resulted in a temporary frameshift, abolished the translation termination codon of the E5a open reading flame (ORF), and introduced a new termination codon in the E40RF. The new uninterrupted E50RF was found to have greater homology to the E40RFs of other papillomaviruses than to their E50RFs. The E50RF of BPV-4 is joined to a five codon ORF in the leader exon of the major transcript, in the same way as the E40RF in the major transcripts of BPV-1 and human papillomavirus type 11. On this basis, it has been redesignated E4, and the viral genomic sequence revised. Two novel transcriptional promoters of BPV-4 were defined: a putative controller of the multiple major RNA start sites around nt 870 and a minor TATA box at nt 691. In addition, minor early region transcripts were mapped which initiate between nt 3071 and nt 3152. None of these transcripts utilizes the splice site at nt 3376. These messages may express E2-encoded functions.

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Section: Protein-coding Potential Of the Minor Transcriptsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Mapping of Two Novel Transcripts of Bovine Papillomavirus Type 4

1988

Journal of General Virology

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“…The generation of the fusion transcripts is a direct consequence of the integration mode of HPV18 DNA in the cells since disruption of the HPV18 early region within the El -E2 segment removes part or all of the 3'-terminal processing signals for maturation of the early mRNAs from the upstream transcribed E6/E6*-E7-El sequences. These 3' signals include a splice acceptor site located in the E2-E4 segment and a polyadenylation signal at the 3'-end of the early transcription unit which has been identified in early mRNAs of BPV1 (Stenlund et al, 1985;Yang et al, 1985), CRPV (Danos et al, 1985;Nasseri and Wettstein, 1984) and HPV6b (Lehn et al, 1984). Integration within El -E2 and synthesis of viralcellular fusion transcripts may be necessary in the malignant tumor cells in order: (i) to disrupt intragenomic regulation mechanisms for HPV gene expression, (ii) to abolish expression of the 3' ORFs of the HPV early transcription unit, and (iii) to stabilize the mRNAs coding for the E6/E6*/E7 proteins thereby assuring expression of the viral functions necessary for maintenance of the malignant phenotype.…”

Section: T T a A Taaggt T G T T A A Ttaggt T G T T A A Ttcgtt T G C Tmentioning

confidence: 99%

Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes.

Schneider-Gädicke¹,

Schwarz²

1986

The EMBO Journal

416

306

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Neuenheimer Feld 280, 6900 Heidelberg, FRG Communicated by H.zur Hausen Transcription of human papillomavirus type 18 (HPV18) DNA in the human cervical carcinoma cell lines HeLa, C41 and SW756 was studied by nucleotide sequence analysis of HPV18-positive cDNA clones isolated from a HeLa, C41 and SW756 cDNA library, respectively, and the cDNA sequences were used to predict the potential encoded proteins. The cDNA clones from all three cell lines were found to be derived from virus-cell fusion transcripts in which 3'-terminal host cell sequences (different for each cell line) were spliced to 5'-tenminal exon sequences from the HPV18 E6-E7-E1 region.Three different types of cDNA clones can be distinguished according to the splicing patterns observed in the 5' tenminal HPV18 sequences. They carry as potential protein-coding regions the HPV18 specific open reading frames E6 and E6* (generated by splicing and identical with E6 up to the E6* splice junction), E7 and El (only in HeLa). Translation of specific cellular genes from the chimeric viral-cellular transcripts seems to be unlikely. The mapping of the 5'-ends of the virus-cell fusion transcripts indicates that transcription is initiated at a viral promoter. The similar patterns of HPV18 transcription in the three different cervical carcinoma cell lines suggest a functional role of HPV18 early genes for the malignant phenotype of these cells.

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“…1 and indicates the positions of the viral open reading frames (ORFs), the sizes and locations of the viral transcripts and some of the relevant regulatory functions. The BPV1 genome is a double-stranded DNA molecule of 7945 nucleotides whose sequence was first determined by Chen et al [6] and by Stenlund et al [7]. It is conventionally shown as a linear map opened at the unique Hpal site with numbering starting at the G of this recognition site.…”

Section: Introductionmentioning

confidence: 99%

“…The BPV-specific polyadenylated RNA transcripts and their sizes as found in transformed cells in culture [35] or in papillomas [13] are shown as arrowed lines below the map. indicates the presence of an extra guanosine residue at position 3444, thus extending the E2 ORF to co-ordinate 3864 [7]. The E2 gene product was initially implicated as a transforming protein, based on mutational analysis [20].…”

Section: Introductionmentioning

confidence: 99%