Messenger RNAs in metaphase II oocytes correlate with successful embryo development to the blastocyst stage

Biase, Fernando H.; Everts, Robin E.; Oliveira, Rosane; Santos-Biase, Weruska Karyna Freitas; Merighe, Giovana Krempel Fonseca; Smith, Lawrence C.; Martelli, Lúcia Regina; Lewin, Harris A.; Meirelles, Flávio Vieira

doi:10.1017/s0967199412000299

Cited by 22 publications

(16 citation statements)

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“…Genes PRPF4B and PXDC1 within this genomic region could be putative candidate genes for female fertility. The expression of PRPF4B in oocytes may provide supports for embryo development in cows 51 . Meanwhile, PRPF4B is also relevant to polycystic ovary in humans, which is a common cause of infertility 52 .…”

Section: Discussionmentioning

confidence: 99%

Genome-wide Association Studies for Female Fertility Traits in Chinese and Nordic Holsteins

Wang

Sahana

et al. 2017

Sci Rep

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Reduced female fertility could cause considerable economic loss and has become a worldwide problem in the modern dairy industry. The objective of this study was to detect quantitative trait loci (QTL) for female fertility traits in Chinese and Nordic Holsteins using various strategies. First, single-trait association analyses were performed for female fertility traits in Chinese and Nordic Holsteins. Second, the SNPs with P-value < 0.005 discovered in Chinese Holsteins were validated in Nordic Holsteins. Third, the summary statistics from single-trait association analyses were combined into meta-analyses to: (1) identify common QTL for multiple fertility traits within each Holstein population; (2) detect SNPs which were associated with a female fertility trait across two Holstein populations. A large numbers of QTL were discovered or confirmed for female fertility traits. The QTL segregating at 31.4~34.1 Mb on BTA13, 48.3~51.9 Mb on BTA23 and 34.0~37.6 Mb on BTA28 shared between Chinese and Nordic Holsteins were further ascertained using a validation approach and meta-analyses. Furthermore, multiple novel variants identified in Chinese Holsteins were validated with Nordic data as well as meta-analyses. The genes IL6R, SLC39A12, CACNB2, ZEB1, ZMIZ1 and FAM213A were concluded to be strong candidate genes for female fertility in Holsteins.

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Section: Discussionmentioning

confidence: 99%

Genome-wide Association Studies for Female Fertility Traits in Chinese and Nordic Holsteins

Wang

Sahana

et al. 2017

Sci Rep

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“…3C). Accumulated maternal mRNA in the oocyte is crucial for successful embryo development prior to activation of the embryonic genome (Biase et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Effective Oocyte Vitrification and Survival Techniques for Bovine Somatic Cell Nuclear Transfer

Park

Lee

Kim

et al. 2015

Cellular Reprogramming

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Bovine somatic cell nuclear transfer (SCNT) using vitrified-thawed (VT) oocytes has been studied; however, the cloning efficiency of these oocytes is not comparable with that of nonvitrified (non-V) fresh oocytes. This study sought to optimize the survival and cryopreservation of VT oocytes for SCNT. Co-culture with feeder cells that had been preincubated for 15 h significantly improved the survival of VT oocytes and their in vitro developmental potential following SCNT in comparison to co-culture with feeder cells that had been preincubated for 2, 5, or 24 h (p<0.05). Spindle assessment via the Oosight Microscopy Imaging System and microtubule staining revealed that vitrified metaphase II oocytes (VT group) were not suitable for SCNT. However, enucleating and/or activating oocytes prior to freezing enhanced their developmental potential and suitability for SCNT. The cloning efficiency of the enucleated-activated-vitrified-thawed (EAVT) group (21.6%) was better than that of the other vitrification groups [enucleated-vitrified-thawed (EVT) group, 13.7%; VT group, 15.0%; p<0.05] and was comparable with that of the non-V group (25.9%). The reactive oxygen species level was significantly lower in the EAVT group than in the other vitrification groups (p<0.05). mRNA levels of maternal genes (ZAR1, BMP15, and NLRP5) and a stress gene (HSF1) were lower in the vitrification groups than in the non-V group (p<0.05), whereas the level of phospho-p44/42 mitogen-activated protein kinase did not differ among the groups. Among the vitrification groups, blastocysts in the EAVT group had the best developmental potential, as judged by their high mRNA expression of developmental potential-related genes (POU5f1, Interferon-tau, and SLC2A5) and their low expression of proapoptotic (CASP3) and stress (Hsp70) genes. This study demonstrates that SCNT using bovine frozen-thawed oocytes can be successfully achieved using optimized vitrification and co-culture techniques.

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“…The transcriptome profile of cumulus cells [ 11 – 19 ] and oocytes [ 20 – 24 ] reflects the developmental potential for successful fertilization and embryo formation. Nevertheless, little is known regarding the connection between the genes expressed in oocytes and cumulus cells.…”

Section: Introductionmentioning

confidence: 99%

Functional signaling and gene regulatory networks between the oocyte and the surrounding cumulus cells

Biase

Kimble

2018

BMC Genomics

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BackgroundThe maturation and successful acquisition of developmental competence by an oocyte, the female gamete, during folliculogenesis is highly dependent on molecular interactions with somatic cells. Most of the cellular interactions identified, thus far, are modulated by growth factors, ions or metabolites. We hypothesized that this interaction is also modulated at the transcriptional level, which leads to the formation of gene regulatory networks between the oocyte and cumulus cells. We tested this hypothesis by analyzing transcriptome data from single oocytes and the surrounding cumulus cells collected from antral follicles employing an analytical framework to determine interdependencies at the transcript level.ResultsWe overlapped our transcriptome data with putative protein-protein interactions and identified hundreds of ligand-receptor pairs that can transduce paracrine signaling between an oocyte and cumulus cells. We determined that 499 ligand-encoding genes expressed in oocytes and cumulus cells are functionally associated with transcription regulation (FDR < 0.05). Ligand-encoding genes with specific expression in oocytes or cumulus cells were enriched for biological functions that are likely associated with the coordinated formation of transzonal projections from cumulus cells that reach the oocyte’s membrane. Thousands of gene pairs exhibit significant linear co-expression (absolute correlation > 0.85, FDR < 1.8 × 10− 5) patterns between oocytes and cumulus cells. Hundreds of co-expressing genes showed clustering patterns associated with biological functions (FDR < 0.5) necessary for a coordinated function between the oocyte and cumulus cells during folliculogenesis (i.e. regulation of transcription, translation, apoptosis, cell differentiation and transport).ConclusionOur analyses revealed a complex and functional gene regulatory circuit between the oocyte and surrounding cumulus cells. The regulatory profile of each cumulus-oocyte complex is likely associated with the oocytes’ developmental potential to derive an embryo.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4738-2) contains supplementary material, which is available to authorized users.

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Messenger RNAs in metaphase II oocytes correlate with successful embryo development to the blastocyst stage

Cited by 22 publications

References 69 publications

Genome-wide Association Studies for Female Fertility Traits in Chinese and Nordic Holsteins

Genome-wide Association Studies for Female Fertility Traits in Chinese and Nordic Holsteins

Effective Oocyte Vitrification and Survival Techniques for Bovine Somatic Cell Nuclear Transfer

Functional signaling and gene regulatory networks between the oocyte and the surrounding cumulus cells

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