MesT, a Unique Epoxide Hydrolase, is Essential for Optimal Growth of
            <i>Mycobacterium Tuberculosis</i>
            in the Presence of Styrene Oxide

Chownk, Manisha; Sharma, Aashish; Singh, Kashmir; Kaur, Jagdeep

doi:10.2217/fmb-2016-0206

Cited by 17 publications

(13 citation statements)

References 35 publications

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“…The possible role of several lipases/esterases in the life of M.tb had been investigated extensively till date. These were involved in the various functions such as synthesis and maintenance of M.tb unique cell envelop, degradation of host cell lipids, xenobiotic degradation, toxic epoxide degradation, evasion and modulation of host immune system, elicit humoral response, dentritic cell maturation and antigen presentation, adaptation during adverse conditions like dormancy and acidification (Deb et al, 2006; Xu et al, 2010; Madan-Lala et al, 2014; Singh et al, 2014c, 2016b; Jadeja et al, 2016; Barisch and Soldati, 2017; Chownk et al, 2017; Johnson, 2017; Kaur et al, 2017a; Kumar et al, 2017b; Vemula et al, 2018). Such variety of functions were possible due to the fact that, these mycobacterial lipolytic enzymes possess many additional activities also like β lactamase, Mycolyltransferase, TDM hydrolase, Eposide Hydrolase, Phospholipase, etc.…”

Section: Introductionmentioning

confidence: 99%

“…Such variety of functions were possible due to the fact that, these mycobacterial lipolytic enzymes possess many additional activities also like β lactamase, Mycolyltransferase, TDM hydrolase, Eposide Hydrolase, Phospholipase, etc. (Zhang et al, 2005; Singh et al, 2016a; Chownk et al, 2017; Queiroz and Riley, 2017). One such activity is mycolyltransferase and TDM hydrolase activity.…”

Section: Introductionmentioning

confidence: 99%

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Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid

Maan

Kumar

Kaur

2018

Front. Cell. Infect. Microbiol.

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Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study. In the present study, Rv1288 and its domains (Est and Lyt) were cloned individually from M.tb into E. coli for expression and purification. The purified rRv1288 and rEst proteins exhibited lipolytic activity with medium chain length esters as optimum substrates, while Lyt domain did not show enzymatic activity. However, presence of Lyt domain resulted in enhanced rate of protein aggregation at higher temperature. Both rRv1288 and rEst followed the similar patterns of substrate specificity, temperature and pH activity. Site directed mutagenesis confirmed the Ser-294, Asp-391 and His-425 as catalytic site residues. Rv1288 was found to be present in cell wall fraction of M.tb H37Ra. Peptidoglycan binding activity of Rv1288 and its domains demonstrated that the Lyt domain is essential for anchoring protein to the cell wall. Expression of rv1288 was up regulated in M.tb under nutrient starved condition. Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall. Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content. Rv1288 also leads to increase in drug resistance of M. smegmatis. Rv1288 also enhanced the intracellular survival of M. smegmatis in Raw264.7 cell line. Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid

Maan

Kumar

Kaur

2018

Front. Cell. Infect. Microbiol.

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“…In addition to the Zur regulon, several genes were induced in p HBA-treated M. tuberculosis that are not induced in PPS mutants ( Table 3 ). Some of these genes are upregulated in hypoxia or after damage to the mycomembrane (23, 24). It is possible these genes are specifically induced in response to extracellular aldehyde exposure rather than endogenously-produced aldehyde as is found in PPS mutants.…”

Section: Resultsmentioning

confidence: 99%

Aldehyde inactivation of the RicR regulon sensitizesMycobacterium tuberculosisto copper

Limón

Samhadaneh

Pironti

et al. 2022

Preprint

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Mycobacterium tuberculosis is a major human pathogen and the causative agent of tuberculosis disease. While M. tuberculosis can persist in the presence of host-derived antimicrobials like nitric oxide and copper, bacteria defective for proteasome activity are highly sensitive to these molecules, making the proteasome an attractive target for drug development. Previous work linked nitric oxide susceptibility with the accumulation of at least one aldehyde in an M. tuberculosis mutant lacking proteasomal degradation. In this study, we show that this aldehyde accumulation is also responsible for copper sensitivity in this strain. Furthermore, we show the exogenous addition of aldehydes to wild-type M. tuberculosis cultures sensitizes bacteria to copper. We determined that aldehydes directly affect the activity of two members of the RicR (regulated in copper repressor) regulon, resulting in the reduced production and function of critical copper-responsive proteins. This study is the first to mechanistically describe how aldehydes can render M. tuberculosis susceptible to an existing host defense, and could support a broader role for aldehydes in controlling M. tuberculosis infections.

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“…kansasii Styrene M . tuberculosis is capable of degrading styrene under hypoxic conditions which may play a role in intracellular survival 54 . Identified previously in breath samples of M .…”

Section: Resultsmentioning

confidence: 99%

Screening of Microbial Volatile Organic Compounds for Detection of Disease in Cattle: Development of Lab-scale Method

Maurer

Ellis

Thacker

et al. 2019

Sci Rep

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The primary hurdle for diagnosis of some diseases is the long incubation required to culture and confirm the presence of bacteria. The concept of using microbial VOCs as “signature markers” could provide a faster and noninvasive diagnosis. Finding biomarkers is challenging due to the specificity required in complex matrices. The objectives of this study were to (1) build/test a lab-scale platform for screening of microbial VOCs and (2) apply it to Mycobacterium avium paratuberculosis ; the vaccine strain of M . bovis Bacillus Calmette-Guérin; and M . kansasii to demonstrate detection times greater those typically required for culture. SPME-GC-MS was used for sampling, sample preparation, and analyses. For objective (1), a testing platform was built for headspace sampling of bacterial cultures grown in standard culture flasks via a biosecure closed-loop circulating airflow system. For (2), results show that the suites of VOCs produced by Mycobacteria ssp. change over time and that individual strains produce different VOCs. The developed method was successful in discriminating between strains using a pooled multi-group analysis, and in timepoint-specific multi- and pair-wise comparisons. The developed testing platform can be useful for minimally invasive and biosecure collection of biomarkers associated with human, wildlife and livestock diseases for development of diagnostic point-of-care and field surveillance.

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MesT, a Unique Epoxide Hydrolase, is Essential for Optimal Growth of Mycobacterium Tuberculosis in the Presence of Styrene Oxide

Cited by 17 publications

References 35 publications

Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid

Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid

Aldehyde inactivation of the RicR regulon sensitizesMycobacterium tuberculosisto copper

Screening of Microbial Volatile Organic Compounds for Detection of Disease in Cattle: Development of Lab-scale Method

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