Meta-Analysis of Anti-Muscarinic Receptor Type 3 Antibodies for the Diagnosis of Sjögren Syndrome

Deng, Chuiwen; Hu, Chaofan; Chen, Si; Li, Jingjing; Wen, Xiaozhong; Wu, Ziyan; Li, Yuanyuan; Zhang, Fengchun; Li, Yongzhe

doi:10.1371/journal.pone.0116744

Cited by 11 publications

(6 citation statements)

References 13 publications

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“…Antinuclear antiboides, anti‐Ro positivity and low complements were more common in Asian populations. Higher sensitivity of anti‐muscarinic receptor type 3 and anti‐α‐fodrin antibodies were reported in Asian patients with pSS . It has also been shown that cryoglobulinemia is less prevalent in Asians .…”

mentioning

confidence: 98%

Primary Sjögren's syndrome in Asia: Yin and Yang?

Sandhya

Danda

2017

Int J of Rheum Dis

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mentioning

confidence: 98%

Primary Sjögren's syndrome in Asia: Yin and Yang?

Sandhya

Danda

2017

Int J of Rheum Dis

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“…However, the functional data have not been reconciled with the sensitivity and specificity of the anti-M3R autoantibodies in screening trials [ 10 ]. Although anti-M3R autoantibodies have been detected in 9–100 % of SS patients depending on the method and antigens used [ 34 ], a recent meta-analysis study concluded that the anti-M3R antibody has high specificity (0.95) but relatively low sensitivity (0.43) to diagnose SS [ 35 ]. In this aspect, the anti-AQP5 autoantibodies identified in the current study could complement anti-M3R autoantibodies.…”

Section: Discussionmentioning

confidence: 99%

Detection of autoantibodies against aquaporin-5 in the sera of patients with primary Sjögren’s syndrome

et al. 2016

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The pathophysiology of exocrine dysfunction observed in Sjögren’s syndrome (SS) is not fully understood. The purpose of this study was to investigate whether autoantibodies against human AQP5 are present in the sera of SS patients. Frozen sections of mouse submandibular salivary glands, CHO cells over-expressing a human AQP5–GFP fusion protein or GFP, and MDCK cells over-expressing AQP5 were used in the indirect immunofluorescence assay to detect anti-AQP5 autoantibodies in the sera from patients with primary SS. The lysates of HEK-293 cells over-expressing the AQP5–GFP fusion protein or GFP were used for immunoprecipitation. Serum IgG from the SS patients but not from the control subjects stained acinar cells in the mouse salivary glands, the signals of which colocalized with those of AQP5-specific antibodies. Serum IgG from the SS patients also selectively stained AQP5–GFP expressed in CHO cells. However, both the control and SS sera immunoprecipitated the AQP5–GFP, suggesting that autoantibodies against AQP5 were also present in the control sera. The screening of 53 control and 112 SS samples by indirect immunofluorescence assay using the AQP5-expressing MDCK cells revealed the presence of significantly higher levels of anti-AQP5 IgG in the SS samples than in the control samples with sensitivity of 0.73 and a specificity of 0.68. Furthermore, the presence of anti-AQP5 autoantibodies was associated with low resting salivary flow in SS patients. In conclusion, anti-AQP5 autoantibodies were detected in the sera from SS patients, which could be a novel biomarker of SS and provide new insight into the pathogenesis of SS.Electronic supplementary materialThe online version of this article (doi:10.1007/s12026-016-8786-x) contains supplementary material, which is available to authorized users.

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“…It is suspected that antibodies towards M3R may potentially inhibit saliva secretion . Some have reported a 60–80% concordance of anti‐M3R with Sjögren's syndrome but the usage has been hampered by reproducibility issues and anti‐M3R is therefore not widely used as a biomarker in clinical settings. Improvement in analytical techniques may change this.…”

Section: Diagnostic Markers In Serum/plasma For Primary Sjögren's Synmentioning

confidence: 99%

Current concepts on Sjögren's syndrome – classification criteria and biomarkers

Jönsson

Brokstad

Jonsson

et al. 2018

European J Oral Sciences

114

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Sjögren's syndrome is a lymphoproliferative disease with autoimmune features characterized by mononuclear cell infiltration of exocrine glands, notably the lacrimal and salivary glands. These lymphoid infiltrations lead to dryness of the eyes (keratoconjunctivitis sicca), dryness of the mouth (xerostomia), and, frequently, dryness of other surfaces connected to exocrine glands. Sjögren's syndrome is associated with the production of autoantibodies because B-cell activation is a consistent immunoregulatory abnormality. The spectrum of the disease extends from an organ-specific autoimmune disorder to a systemic process and is also associated with an increased risk of B-cell lymphoma. Current treatments are mainly symptomatic. As a result of the diverse presentation of the syndrome, a major challenge remains to improve diagnosis and therapy. For this purpose an international set of classification criteria for primary Sjögren's syndrome has recently been developed and validated and seems well suited for enrolment in clinical trials. Salivary gland biopsies have been examined and histopathology standards have been developed, to be used in clinical trials and patient stratification. Finally, ultrasonography and saliva meet the need of non-invasive imaging and sampling methods for discovery and validation of disease biomarkers in Sjögren's syndrome.

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Meta-Analysis of Anti-Muscarinic Receptor Type 3 Antibodies for the Diagnosis of Sjögren Syndrome

Cited by 11 publications

References 13 publications

Primary Sjögren's syndrome in Asia: Yin and Yang?

Primary Sjögren's syndrome in Asia: Yin and Yang?

Detection of autoantibodies against aquaporin-5 in the sera of patients with primary Sjögren’s syndrome

Current concepts on Sjögren's syndrome – classification criteria and biomarkers

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