Meta-Analysis of Bovine Digital Dermatitis Microbiota Reveals Distinct Microbial Community Structures Associated With Lesions

Caddey, Ben; Buck, Jeroen De

doi:10.3389/fcimb.2021.685861

Cited by 17 publications

(20 citation statements)

References 49 publications

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“…Most feet were positive for Fusobacterium at multiple timepoints, including samples from mock inoculated and from samples obtained prior to inoculation with lesion material (week 0). While Fusobacterium is a known contributor to foot rot of cattle and small ruminants, its role in DD is not well defined and is controversial ( 11 , 41 , 48 – 54 ). Our data supports an ambiguous role for Fusobacterium in TAHD as most sheep feet, even in the mock inoculated treatment group, were positive for Fusobacterium prior to inoculation and many in the control group did not develop lesions.…”

Section: Resultsmentioning

confidence: 99%

“…Comparing mock to inoculated tissues at three timepoints, 86 OTUs were increased in inoculated samples with the majority found in all three sampling (43 OTUs) timepoints but the highest number was found at week 2 ( Figure 7 ). Others have reported that microbial diversity of DD lesions decreases with time, with treponemes eventually becoming the dominant organism ( 31 , 41 , 44 , 57 ). This was not observed in the current study.…”

Section: Discussionmentioning

confidence: 99%

“…T. medium, T. pedis, and T. phagedenis have consistently been found within TAHD lesions (2, 3) as well as within bovine DD lesions (2,3,15,31,(39)(40)(41)(42)(43)(44)(45)(46)(47). Using species specific primers, one or more of these species were identified on inoculated foot swabs in at least one sampling timepoint (Table 2).…”

Section: Pcrmentioning

confidence: 99%

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Lesion Material From Treponema-Associated Hoof Disease of Wild Elk Induces Disease Pathology in the Sheep Digital Dermatitis Model

et al. 2022

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A hoof disease among wild elk (Cervus elaphus) in the western United States has been reported since 2008. Now present in Washington, Oregon, Idaho, and California, this hoof disease continues to spread among elk herds suggesting an infectious etiology. Causing severe lesions at the hoof-skin junction, lesions can penetrate the hoof-horn structure causing severe lameness, misshapen hooves, and in some cases, sloughed hooves leaving the elk prone to infection, malnutrition, and premature death. Isolated to the feet, this disease has been termed treponeme-associated hoof disease due to the numerous Treponema spp. found within lesions. In addition to the Treponema spp., treponeme-associated hoof disease shares many similarities with digital dermatitis of cattle and livestock including association with several groups of anaerobic bacteria such as Bacteroides, Clostridia, and Fusobacterium, neutrophilic inflammatory infiltrate, and restriction of the disease to the foot and hoof tissues. To determine if there was a transmissible infectious component to this disease syndrome, elk lesion homogenate was used in a sheep model of digital dermatitis. Ten animals were inoculated with lesion material and lesion development was followed over 7 weeks. Most inoculated feet developed moderate to severe lesions at 2- or 4-weeks post-inoculation timepoints, with 16 of 18 feet at 4 weeks also had spirochetes associated within the lesions. Histopathology demonstrated spirochetes at the invading edge of the lesions along with other hallmarks of elk hoof disease, neutrophilic inflammatory infiltrates, and keratinocyte erosion. Treponema-specific PCR demonstrated three phylotypes associated with elk hoof disease and digital dermatitis were present. Serum of infected sheep had increased anti-Treponema IgG when compared to negative control sheep and pre-exposure samples. Analysis of the bacterial microbiome by sequencing of the bacterial 16S rRNA gene showed a community structure in sheep lesions that was highly similar to the elk lesion homogenate used as inoculum. Bacteroidies, Fusobacterium, and Clostridia were among the bacterial taxa overrepresented in infected samples as compared to negative control samples. In conclusion, there is a highly transmissible, infectious bacterial component to elk treponeme-associated hoof disease which includes several species of Treponema as well as other bacteria previously associated with digital dermatitis.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Lesion Material From Treponema-Associated Hoof Disease of Wild Elk Induces Disease Pathology in the Sheep Digital Dermatitis Model

et al. 2022

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“…При развитии болезни из пораженных мест выделяется большое разнообразие микроорганизмов [3,6]. По данным авторов [5] такие бактерии как трепонемы, микоплазмы, порфиромонады и/или фузобактерии могут играть ключевую роль в патогенезе пальцевого дерматита.…”

Section: Introductionunclassified

“…По разным литературным источникам частота встречаемости пальцевого дерматита может достигать до 80% среди поголовья крупного рогатого скота. Чаще распространенность данной болезни составляет около 20% в стаде [1,3,5].…”

Section: Introductionunclassified

Prevalence of mortellaro disease in cows, taking into account lactation (treatment and prevention)

Semenov

Kuznetsova

Nazarova

et al. 2022

jour

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Digital dermatitis, Mortellaro's disease, is one of the most common diseases of the hooves of cattle on farms, which can lead to significant economic losses. Digital dermatitis is considered a polybacterial disease in which the microbiome composition changes as the different stages of the disease progress. According to the literature, bacteria such as treponemas, mycoplasmas, porphyromonas and/or fusobacteria may play a key role in the pathogenesis of digital dermatitis. Cattle digital dermatitis has a staging ranging from minor ulcerations to hyper- and dyskeratic proliferation of the papillary layer of the skin base. The foci are localized on the palmar or plantar surface of the fingers and hooves, between the fingers, along the perimeter of the corolla, under the rudimentary fingers and above the horn capsule. The article evaluates the frequency of its occurrence, the impact on the productivity of dairy cows and compares the effectiveness of the treatment of Mortellaro's disease with the use of beech tar and Terramycin spray. In the studied livestock, digital dermatitis was detected in 1.5% of animals. Most often, digital dermatitis of various stages was identified in cows of II, III, IV and more lactation. A decrease in the average daily milk yield in cows with Mortellaro disease was determined - 21.78 kg of milk, while the average value for the household is 24 kg. Also, the insemination of 14 cows out of 18 sick animals after the first insemination 42.9% were barren. In 90% of the animals, the pelvic limbs were affected, pathological foci were localized in the heel area in 74% of cases, in the interhoof arch in 23%, and in the corolla in 3%. In 78% of the animals one limb was affected. Surgical removal of the affected tissues, the use of a bandage with beech tar reduced the treatment time by 5-7 days compared with the use of Terramycin spray.

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Skin microbiota dynamics of dairy cows affected by digital dermatitis

Ariza

Döpfer

Anklam

et al. 2019

Preprint

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14Bovine digital dermatitis (DD), is a highly prevalent disease among dairy cattle 15 characterized by ulcerative and painful lesions. While multiple risk factors are involved 16 in the disease, its precise etiology remains unclear and the effectiveness of the current 17 control strategies still highly variable. However, and even if the main role of Treponema 18 spp. in the development of the disease is consistently recognized, the importance of 19 other bacterial communities and the factors affecting the skin environment remains 20 uncertain. The objective of this study was to investigate the dynamics of microbiotas 21 from feet affected by DD, before and after the implementation of disinfectant footbaths 22 under field conditions. During this investigation, the diversity, structure, and 23 composition of microbiotas from DD lesions over time were explored according to 24 different clinical factors. The findings of this investigation confirmed a particular 25 lesions evidenced a different structure and diversity in comparison to non-proliferative 30 lesions. The composition of microbiotas changed over time revealing the potential main 31 role of Treponema spp., Fusobacterium spp., Mycoplasma spp. and Porphyromonas 32 spp. in the dynamics of DD lesion progression. Further studies are necessaries to 33 confirm if the clinical evolution of DD lesions is driven by a particular microbiota and 34 how this microbiota induces disease. 35 Highlights 39Multiple bacteria have been identified in DD lesions. However, a large part of these 40 microorganisms are inhabitants of the foot skin and the farm environment. For the first 41 time, the microbiotas of DD lesions were followed over 45 days under field conditions 42 to explore their evolution over time and how footbaths practices may affect their 43 dynamics. The results of this investigation confirmed a particular microbiota related to 44 DD lesions dominated by Treponema spp. and highly different from those microbiotas 45 of healthy skin. The microbiotas from DD lesions evolved over the study period and 46 the differential bacteria were identified. Further studies are guaranteed to determine 47 the role of the bacteria composing these microbiotas on the lesion occurrence and its 48 outcome. 49 50 polymicrobial disease spread across the world and characterized by ulcerative and 55 painful lesions which might persist as a chronic condition. DD lesions progress in 56 dynamic ways conditioned by multiple environmental factors that influence the spread 57 of associated-pathogens and the integrity of the foot skin. Currently, the control 58 strategies implemented in dairy farms such as footbaths or individual treatments, have 59 demonstrated variable effectiveness in the healing and prevention of DD lesions 2,3 . 60Although the precise etiology of DD remains unclear, the maceration of the foot skin 61 and the presence of Treponema spp. have been consistently identified as the major 62 etiological components of DD 4-7 . However, multiple other bacteria have also been 63 associa...

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Meta-Analysis of Bovine Digital Dermatitis Microbiota Reveals Distinct Microbial Community Structures Associated With Lesions

Cited by 17 publications

References 49 publications

Lesion Material From Treponema-Associated Hoof Disease of Wild Elk Induces Disease Pathology in the Sheep Digital Dermatitis Model

Lesion Material From Treponema-Associated Hoof Disease of Wild Elk Induces Disease Pathology in the Sheep Digital Dermatitis Model

Prevalence of mortellaro disease in cows, taking into account lactation (treatment and prevention)

Skin microbiota dynamics of dairy cows affected by digital dermatitis

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