Meta-analysis of RNA-seq studies reveals genes responsible for life stage-dominant functions in <i>Schistosoma mansoni</i>

Lu, Zhigang; Berriman, Matthew

doi:10.1101/308189

Cited by 13 publications

(15 citation statements)

References 52 publications

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“…Transcript profiling by RNA-seq showed high transcript levels of Sm-tdc in bM compared to sM and no transcripts in bF or sF. The meta-analysis of all life stages showed that Sm-tdc is stage- and gender-specific expressed in males, being significantly up-regulated after pairing (Lu and Berriman, 2018). Similarly, the amount of Sm-ebony transcripts dominated in bM but also in sF, whereas sM and bF showed significantly reduced transcript levels.…”

Section: “Nervous” Male Schistosomementioning

confidence: 99%

“…Similarly, the amount of Sm-ebony transcripts dominated in bM but also in sF, whereas sM and bF showed significantly reduced transcript levels. The meta-analysis showed stage-specific expression in adults with a significant up-regulation in bM and sF (Lu and Berriman, 2018). A comprehensive quantitative reverse transcription-PCR analysis with RNA from males after pairing, separation, and re-pairing in vitro finally confirmed the direct influence of pairing on the transcript levels of both genes with a clear bias toward bM (Haeberlein et al, 2019).…”

Section: “Nervous” Male Schistosomementioning

confidence: 99%

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Males, the Wrongly Neglected Partners of the Biologically Unprecedented Male–Female Interaction of Schistosomes

Spänig

Weth

et al. 2019

Front. Genet.

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Schistosomes are the only platyhelminths that have evolved separate sexes, and they exhibit a unique reproductive biology because the female’s sexual maturation depends on a constant pairing contact with the male. In the female, pairing leads to gonad differentiation, which is associated with substantial morphological changes, and controls among others the expression of gonad-associated genes. In the male, no morphological changes have been observed after pairing, although first data indicated an effect of pairing on gene transcription. Comprehensive transcriptomic approaches have revealed an unexpected high number of genes that are differentially transcribed in the male after pairing. Their identities suggest roles for the male that are not restricted to feeding and enhanced muscular power to transport paired female and, as assumed before, to induce its sexual maturation by one “magic” factor. Instead, a more complex picture emerges in which both partners live in a reciprocal sender-recipient relationship that not only affects the gonads of both genders but may also involve tactile stimuli, transforming growth factor β signaling, nutritional parts, and neuronal processes, including neuropeptides and G protein-coupled receptor signaling. This review provides a summary of transcriptomics including an overview of genes expressed in a pairing-dependent manner in schistosome males. This may stimulate further research in understanding the role of the male as the recipient of the female’s signals upon pairing, the male’s “capacitation,” and its subsequent competence as a sender of information. The latter process finally transforms a sexually immature, autonomous female without completely developed gonads into a sexually mature, partially non-autonomous female with fully differentiated gonads and enormous egg production capacity.

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Section: “Nervous” Male Schistosomementioning

confidence: 99%

Section: “Nervous” Male Schistosomementioning

confidence: 99%

Males, the Wrongly Neglected Partners of the Biologically Unprecedented Male–Female Interaction of Schistosomes

Spänig

Weth

et al. 2019

Front. Genet.

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“…A recent meta-analysis of published RNA-seq experiments provides a comprehensive view of gene expression changes across all life stages of Schistsoma mansoni (Lu and Berriman 2018). However, as it's not possible to cover all genes in one publication, it's very likely that there are more interesting candidates that were not mentioned in the analysis.…”

Section: Introductionmentioning

confidence: 99%

A web portal for gene expression across all life stages of Schistosoma mansoni

Zhang

Berriman

2018

Preprint

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RNA-seq approach can provide useful information about gene expression. Although several studies have been conducted in the parasite Schistosoma mansoni, the gene expression data is often limited to differential analysis between certain life stages. A recent meta-analysis of RNA-seq studies generated valuable expression data across all life stages of S. mansoni. To facilitate the use and visualisation of these data, we established an interactive web portal implementing not only data from above-mentioned analysis, but also functional aspects including conserved domains and associated pathways, as a complement to main databases for S. mansoni. Users can also visualise and analyse their own data via the web portal. The interactive visualisation implemented in the web portal can facilitate characterising schistosome genes for the research community.

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“…This enzyme domain shows homology with the human ADP ribose (ADPR) pyrophosphatase NUDT9, a feature characteristic of TRMP2 channels [14][15][16][17]. Analysis of Sm.TRPM PZQ in transcriptomic datasets evidences expression across various parasite life cycle stages [18], and analysis of flatworm genomes revealed expression of closely related TRPM channels in both free-living and parasitic flatworms that exhibit sensitivity to PZQ ( Supplementary Figure 3).…”

Section: Resultsmentioning

confidence: 99%

“…Finally, consistent with Sm.TRPM PZQ being a target of this clinically important therapeutic, Sm.TRPM PZQ is expressed through various parasite life cycle stages [18] and is present in other flatworms sensitive to PZQ ( Supplementary Figure 3).…”

Section: Discussionmentioning

confidence: 99%