Meta-analysis of the clinical value of Astragalus membranaceus in diabetic nephropathy

Li, Mingxin; Wang, Weixin; Xue, Jun; Gu, Yong; Lin, Song

doi:10.1016/j.jep.2010.10.012

Cited by 120 publications

(96 citation statements)

References 11 publications

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“…It has been reported that A. membranaceus Bge mitigates urine protein levels and exerts a protective effect on kidney function in patients with CKD (Chen, 2001;Li et al, 2011). Astragaloside IV, one of the major active components of A. membranaceus, has been detailed in various studies.…”

Section: Discussionmentioning

confidence: 99%

Astragaloside IV Ameliorates Renal Fibrosis via the Inhibition of Mitogen-Activated Protein Kinases and Antiapoptosis In Vivo and In Vitro

Xu¹,

Shao²,

Tian³

et al. 2014

J Pharmacol Exp Ther

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Section: Discussionmentioning

confidence: 99%

Astragaloside IV Ameliorates Renal Fibrosis via the Inhibition of Mitogen-Activated Protein Kinases and Antiapoptosis In Vivo and In Vitro

Xu¹,

Shao²,

Tian³

et al. 2014

J Pharmacol Exp Ther

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“…Bunge (Fabaceae) are folkloric Chinese herbs and are used traditionally for treatment of kidney diseases. In the traditional system of medicine, extracts of the roots of A. membranaceus are used alone or in combination with extracts of A. sinensis roots to treat patients with chronic kidney disease (Li et al, 2011;Zhong et al, 2013). Previous studies by using in vivo animal models have shown their protective benefits against ischaemia-reperfusion injury (Cai et al, 2001), unilateral urinary obstruction and fibrosis in rat (Wojcikowski et al, 2010), chronic puromycinaminonuclease nephrosis (Wang et al, 2004), and improved renal microvascular insufficiency in nephrectomized rats (Song et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Protection against oxidative stress-induced apoptosis in kidney epithelium by Angelica and Astragalus

Shahzad

Small

Morais

et al. 2016

Journal of Ethnopharmacology

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Ethnopharmacological relevance: Astragalus membranaceus either alone or in combination withAngelica sinensis has been used traditionally for kidney disease in East Asia and China for thousands of years. Previous studies using in vivo animal models have shown the benefits of these medicinal herbs in kidney diseases that involve oxidative stress. However, the mechanisms by which these medicinal herbs protect kidney cells remain largely unknown.Aim of the study: To investigate the mechanisms by which ethanol, methanol and aqueous crude extracts of roots of A. membranaceus and A. sinensis afford protection to human kidney proximal tubular epithelial cells, using an in vitro model of oxidative stress. Materials and Methods:Ethanol, methanol and aqueous extracts of roots of A. membranaceus and A. sinensis were prepared by a three-solvent sequential process. HK2 human kidney proximal tubular epithelial cells were treated with H 2 O 2 alone (0.5 mM) or in combination with different concentrations of extracts. Cell mitosis and death (microscopy) and cell viability (MTT assay) were compared. Western immunoblot was used to study expression of apoptosis-related proteins (pro-apoptotic Bax andanti-apoptotic Bcl-X L ), and cell survival (NFκB subunits p65 and p50), pro-inflammatory (TNF-α) and protective (TGFβ1) proteins.Results: H 2 O 2 -induced oxidative stress significantly increased apoptosis and reduced cell survival; upregulated pro-apoptotic and down-regulated Bcl-X L ; increased NFκB (p65, p50); increased TNFα; and decreased TGFβ1. All changes indicated kidney damage and dysfunction.All were modulated by all extracts of both plant species, except for NFκB which was only modulated by extracts of A. membranaceus. Conclusions:In conclusion, in a model of oxidative stress that might occur after nephrotoxicity, the plant extracts were protective via anti-apoptotic and anti-inflammatory mechanisms.3

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“…Astragalus membranaceus is one of the most common traditional Chinese medicines used in clinical treatment by the oral administration to treat a wide variety of diseases and body disorders [38-40] . As-IV is a monomer located in an extract of Astragalus membranaceus [41].…”

Section: Discussionmentioning

confidence: 99%

Astragaloside-IV Protects Against Heat-induced Apoptosis by Inhibiting Excessive Activation of Mitochondrial Ca2+ Uniporter

Dong

Zhang

Chen

et al. 2017

Cell Physiol Biochem

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Background: Heat causes airway damage during inhalation injury because of bronchial epithelial cell damage. Accumulating evidence shows that mitochondrial uniporter (MCU) is involved in cell damage. We investigated the MCU activity after heat treatment and assessed whether Astragaloside-IV (AS-IV) suppresses heat-induced apoptosis in bronchial epithelial cells by inhibiting the activation of the mitochondrial Ca²⁺ uniporter (MCU), mitochondrial depolarisation and reactive oxygen species (ROS) production. Methods: The bronchial epithelial cell line 16HBE14o- was heat treated, and cell apoptosis was induced in vitro and in vivo. AS-IV was inorganically administered to Wistar rats twice a day after thermal inhalation injury, and 16HBE140- cells were treated with AS-IV after incubation at 47°C for 5 min. Protein expression was determined using Western blotting and commercial kits, apoptosis with TUNEL staining, mitochondrial channel activity by patch clamp, reactive oxygen species by MitoSOX^TM fluorescence, ATP levels and enzyme activities by commercial kits as well as mitochondrial respiration and calcium by fluorescence. Results: AS-IV markedly inhibited heat-induced apoptosis, as indicated by the increased expression of the pro-apoptotic genes Bak, Bik and Bmf and increased expression of the apoptosis markers Bax, cleaved parp, cleaved caspase3 and cytochrome C. We found that MCU activation promoted mitochondrial Ca²⁺ overload, ATP depletion, mitochondrial ROS production and cytochrome c release and rapidly induced apoptosis. However, AS-IV treatment reduced excessive MCU activation and led to resistance against mitochondrial Ca²⁺ overload and excessive cytochrome C release; these effects were blocked by the MCU activator spermine. AS-IV treatment elevated ATP production and decreased ROS activity. Conclusions: MCU plays crucial roles in heat-induced mitochondrial apoptosis in 16HBE140- cells, suggesting a potential target for AS-IV treatment.

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Meta-analysis of the clinical value of Astragalus membranaceus in diabetic nephropathy

Cited by 120 publications

References 11 publications

Astragaloside IV Ameliorates Renal Fibrosis via the Inhibition of Mitogen-Activated Protein Kinases and Antiapoptosis In Vivo and In Vitro

Astragaloside IV Ameliorates Renal Fibrosis via the Inhibition of Mitogen-Activated Protein Kinases and Antiapoptosis In Vivo and In Vitro

Protection against oxidative stress-induced apoptosis in kidney epithelium by Angelica and Astragalus

Astragaloside-IV Protects Against Heat-induced Apoptosis by Inhibiting Excessive Activation of Mitochondrial Ca2+ Uniporter

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