Meta-analysis reveals benefits of co-inoculation of soybean with Azospirillum brasilense and Bradyrhizobium spp. in Brazil

Barbosa, Julierme Zimmer; Hungría, Mariangela; Sena, João Victor da Silva; Pôggere, Giovana Clarice; Reis, André Rodrigues dos; Corrêa, Rodrigo Studart

doi:10.1016/j.apsoil.2021.103913

Cited by 62 publications

(47 citation statements)

References 73 publications

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“…and common bean (Phaseolus vulgaris L.) (Hungria et al 2013(Hungria et al , 2016Santos et al 2021). In ten years, more than 200 studies have been published with strains CNPSo 2083 and CNPSo 2084 (Barbosa et al 2021;Santos et al 2021) and plant-growth promotion has been associated mainly with the high levels of phytohormones synthesized by the strains, with an emphasis on indole-3-acetic acid (IAA) (Fukami et al 2017), greatly improving root growth and the uptake of water and nutrients. The strains also contribute via the biological nitrogen fixation process, and induction of mechanisms of resistance to abiotic and biotic stresses (Fukami et al 2018b;Santos et al 2021), and the genes for such properties were confirmed in their genomes (Hungria et al 2018).…”

Section: Discussionmentioning

confidence: 99%

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Seed and leaf-spray inoculation of PGPR in brachiarias (Urochloa spp.) as an economic and environmental opportunity to improve plant growth, forage yield and nutrient status

et al. 2021

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Purpose Brazil has 180 Mha of pastures, 86 Mha occupied with Urochloa spp. (syn. Brachiaria), and 70% in some level of degradation. Inoculation with plant-growth-promoting bacteria (PGPR) may represent an economic and environmental feasible strategy to improve pasture production. Methods Two greenhouse and seven field trials were performed to verify the effects of seed inoculation at sowing or leaf-spray inoculation in established pastures of Urochloa with elite strains of Azospirillum brasilense and Pseudomonas fluorescens. All plants received nutrients including 40 kg ha−1 of N at sowing, and half of the treatments a supply of 40 kg ha−1 of N 30 days after emergence. Results A. brasilense increased shoot biomass by an average of 16.8% with both seed and leaf-spray inoculation, whereas P. fluorescens increased by 15.2 and 14.2%, respectively, always higher with the extra supply of N. Seed and leaf-spray inoculation with A. brasilense increased N content by an average of 11.7 and 20.7%, and K by 9.9 and 11.3%, respectively; for P. fluorescens average increases were of 33.3 and 36.6% for P, and of 10.6 and 13.6% for K, respectively. Benefits were mainly attributed to improvements in root architecture by the synthesis of phytohormones. Biological nitrogen fixation in A. brasilense, P acquisition (solubilization of phosphates and siderophores synthesis) and ACC-deaminase in P. fluorescens also contributed to plant growth and nutrient status. Conclusion Inoculation with elite PGPR strains translated into more fodder and improved nutritional value of feed for livestock, representing a promising and environmentally-friendly strategy for tropical pastures.

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Section: Discussionmentioning

confidence: 99%

“…In implanted pastures, there is also the limitation of inoculant application, as the only viable method would be via leaf-spray. However, it is worth mentioning that there are positive indications of success of leaf-spray of A. brasilense in maize (Fukami et al 2016;Barbosa et al 2021), encouraging to verify the feasibility on pastures.…”

Section: Introductionmentioning

confidence: 85%

Seed and leaf-spray inoculation of PGPR in brachiarias (Urochloa spp.) as an economic and environmental opportunity to improve plant growth, forage yield and nutrient status

et al. 2021

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“…For common beans, individual inoculation with R. tropici boosted yield by 8.3%, but the addition of A. brasilense raised this gure to 19.6%. A metadata analysis by Barbosa et al (2021) revealed that soybean co-inoculation was related to a 2.8% increase in grain yield and a 3.2% increase in N concentration in the grains with respect to the inoculation only with Bradyrhizobium. In other 37 eld trials, soybean co-inoculation with Bradyrhizobium and A. brasilense also increased mean yield by 227 kg ha −1 with respect to the inoculation with Bradyrhizobium alone and by 335 kg ha −1 with respect to the uninoculated control ).…”

Section: Discussionmentioning

confidence: 99%

The Interaction between Bradyrhizobium Japonicum E109 and Azospirillum Brasilense Az39 Improves Bradyrhizobium-Soybean Symbiosis: The Secrets Behind Co-Inoculation

Torres

Donadío

López

et al. 2021

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Aims The aim of this work was evaluating the interaction between two of the most widely used strains for soybean inoculation in Argentina, B. japonicum E109 (BjE109) and A. brasilense Az39 (AbAz39). Methods Five treatments were performed: (i) uninoculated seeds; (ii) seeds inoculated with BjE109; (iii) seeds inoculated with AbAz39; (iv) seeds co-inoculated with BjE109 and AbAz39 in a 1:1 proportion (BjE109 + AbAz39) at the seeds sowing and (v) seeds inoculated with a 1:1 proportion of BjE109 and AbAz39 (BjE109-AbAz39) 24 h before seeds sowing. Each treatment was assessed through a seed recovery assay, glasshouse assays and field assays. Results The combination between the two strains improved the ability of BjE109 to survive on soybean seeds after inoculation partially explained by AbAz39’s capacity to produce diverse biologically active molecules. As a result of the greater rhizobial survival on seeds the nodulation values and symbiosis parameters like nodule number, size and biomass and nodulation percentage also increased. In agreement with these observations, combining BjE109 and AbAz39 at strains the grain yield under field conditions were 13,3 and 17,3% greater than single BjE109 inoculation. Conclusions These results here show that the pre-culture combining BjE109 and AbAz39 before the inoculation to the soybean seeds has benefits in plant nodulation and hence production, more than individual inoculation with BjE109 or AbAz39, or the immediate co-inoculation of both strains.

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“…Increasing success has been obtained after the deployment of the technology of co-inoculation with Bradyrhizobium spp. and A. brasilense in 2013, with doubled grain yield benefits culminating in the commercial launch of a technological package containing the two bacteria separately (Hungria et al 2013b , 2015 ; Barbosa et al 2021 ; Santos et al 2021 ). In a recent a meta-analysis of 48 publications covering field trials at 38 different locations in Brazil, Barbosa et al ( 2021 ) detected statistically significant increases in grain yield and other plant growth parameters due to the co-inoculation.…”

Section: Discussionmentioning

confidence: 99%

“…and A. brasilense in 2013, with doubled grain yield benefits culminating in the commercial launch of a technological package containing the two bacteria separately (Hungria et al 2013b , 2015 ; Barbosa et al 2021 ; Santos et al 2021 ). In a recent a meta-analysis of 48 publications covering field trials at 38 different locations in Brazil, Barbosa et al ( 2021 ) detected statistically significant increases in grain yield and other plant growth parameters due to the co-inoculation. In addition, the main published studies in Brazil were recently compiled by Santos et al ( 2021 ), and as an example, the studies of Ferri et al ( 2017 ) and Galindo et al ( 2018 ) indicated increases in grain yield due to the co-inoculation of 20.3 and 11.2%, respectively, compared to the inoculation exclusively with Bradyrhizobium .…”

Section: Discussionmentioning

confidence: 99%

Combining microorganisms in inoculants is agronomically important but industrially challenging: case study of a composite inoculant containing Bradyrhizobium and Azospirillum for the soybean crop

2021

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The increasing global perception of the importance of microbial inoculants to promote productivity and sustainability in agriculture prompts the adoption of bio-inputs by the farmers. The utilization of selected elite strains of nitrogen-fixing and other plant-growth promoting microorganisms in single inoculants creates a promising market for composite inoculants. However, combining microorganisms with different physiological and nutritional needs requires biotechnological development. We report the development of a composite inoculant containing Bradyrhizobium diazoefficiens and Azospirillum brasilense for the soybean crop. Evaluation of use of carbon sources indicates differences between the microbial species, with Bradyrhizobium growing better with mannitol and glycerol, and Azospirillum with malic acid and maleic acid, allowing the design of a formulation for co-culture. Species also differ in their growth rates, and the best performance of both microorganisms occurred when Azospirillum was inoculated on the third day of growth of Bradyrhizobium. The composite inoculant developed was evaluated in five field trials performed in Brazil, including areas without and with naturalized populations of Bradyrhizobium. The composite inoculant resulted in symbiotic performance comparable to the application of the two microorganisms separately. In comparison to the single inoculation with Bradyrhizobium, co-inoculation resulted in average increases of 14.7% in grain yield and 16.4% in total N accumulated in the grains. The performance of the composite inoculant was similar or greater than that of the non-inoculated control receiving a high dose of N-fertilizer, indicating the importance of the development and validation of inoculants carrying multiple beneficial microorganisms.

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Meta-analysis reveals benefits of co-inoculation of soybean with Azospirillum brasilense and Bradyrhizobium spp. in Brazil

Cited by 62 publications

References 73 publications

Seed and leaf-spray inoculation of PGPR in brachiarias (Urochloa spp.) as an economic and environmental opportunity to improve plant growth, forage yield and nutrient status

Seed and leaf-spray inoculation of PGPR in brachiarias (Urochloa spp.) as an economic and environmental opportunity to improve plant growth, forage yield and nutrient status

The Interaction between Bradyrhizobium Japonicum E109 and Azospirillum Brasilense Az39 Improves Bradyrhizobium-Soybean Symbiosis: The Secrets Behind Co-Inoculation

Combining microorganisms in inoculants is agronomically important but industrially challenging: case study of a composite inoculant containing Bradyrhizobium and Azospirillum for the soybean crop

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