Meta-Topolin mediated in vitro propagation in an ornamentally important crop Iris × hollandica Tub. cv. professor Blaauw and genetic fidelity studies using SCoT markers

Abstract

Dutch Iris is a commercially important bulbous ornamental crop. Its high demand in global oriculture market necessitates the production of high-quality planting material. In the present investigation, the signi cance of meta-topolin (mT), a novel aromatic cytokinin, was studied on in vitro morphogenesis of Iris × hollandica Tub. cv. Professor Blaauw (Dutch Iris). Cytokinin mT (1.0 mg L − 1 ) as a better substitute to BAP (6-Benzylaminopurine) and Kn (Kinetin), has resulted in maximum shoot induction response (86.11 per cent) and increased emergence of micro shoots (4.95 shoots/explant with average shoot length of 5.65 cm) from twin scale explants. Photoperiod also affected the shoot induction, wherein initial 1-week (w) dark incubation signi cantly increased shoot induction response (94.44 per cent). Both meta-topolin and initial 1w dark incubation resulted in improved quality of regenerated shoots by reducing the incidence of necrosis and preventing vitri cation. The collective consequence of cytokininauxin (1.0 mg L − 1 mT + 0.25 mg L − 1 NAA) showed considerably higher number of shoots (17.53) with mean shoot length (6.90 cm) and maximum number of bulbets (2.74). Positive effect of increased sucrose concentration (90 g L 1 ) and paclobutrazol (5 mg L 1 ) on in vitro bulblet formation and bulblet size was also observed. The superiority of mT over BAP was also found during in vitro rhizogenesis. Shoots derived from mT medium were healthy and long enough, thus showed better rooting response (63.3 %) on ½ MS medium + 0.5 mg L − 1 IAA after 4 weeks. The mT raised plantlets were acclimatized in a greenhouse and showed 89.6 % survival under ex vitro conditions The clonal delity of the in vitro regenerated plants was evaluated using Start Codon Targeted (SCoT) markers. Out of 36 primers, 13 primers showed clearly scorable monomorphic bands, thus displaying genetic uniformity among in vitro regenerated plantlets. This meta-topolin mediated protocol can be routinely used for the rapid large scale production of this valuable oriculture crop.

Application of machine learning in in vitro propagation of endemic Lilium akkusianum R. Gämperle

“…cv. professor Blaauw, MS + 1.0 mg/L mT + 0.25 mg/L NAA resulted in a considerably higher shoot number per explant with 17.53 and a mean shoot length of 7.06 cm [ 27 ]. In agreement with previous studies, MS supplemented with 2.50 mg/L mT with 0.1 mg/L NAA enhanced the formation of healthy and uniformly grown shoots per node and better shoot length in Withania coagulans (Stocks) Dunal [ 45 ].…”

“…It has been indicated that difficulties like hyperhydricity, shoot-tip necrosis, poor rooting ability, and early senescence while creating effective micropropagation protocols can be alleviated using mT alternative to BA [ 24 ]. Additionally, positive effects of mT in micropropagation have been proven in shoot regeneration of Daphne mezereum [ 25 ], Gerbera jamesonii [ 26 ], Iris × hollandica [ 27 ], and Tulipa sp. L. [ 28 ], Hypericum sp.…”