Metabolic activation of the mutagen azide in biological systems

Owais, W.M.; Kleinhofs, A.

doi:10.1016/0027-5107(88)90101-7

Cited by 76 publications

(40 citation statements)

References 62 publications

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“…NaN 3 causes point mutations in several organisms including bacteria, plants and animals [39][40][41]. A lot of studies showed that mutagenesis mechanism of NaN 3 is associated with its metabolite called L-azidoline [40][41][42]. It can be seen that the lichen extracts inhibit mutagenic activity of NaN 3 on Z. mays seed germination.…”

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confidence: 93%

Mutagenic and antimutagenic properties of some lichen species grown in the Eastern Anatolia Region of Turkey

et al. 2012

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confidence: 93%

Mutagenic and antimutagenic properties of some lichen species grown in the Eastern Anatolia Region of Turkey

et al. 2012

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“…In Salmonella typhimurium sodium azide reverts only base substitution mutants; the mutation process is facilitated in strains deficient in excision-repair mechanisms (4). The first step in the azide mutagenic pathway in barley and Salmonella has been elucidated: O-acetylserine (thiol)-lyase converts O-acetylserine with azide to ,-azidoalanine; azide substitutes for the natural sulfide substrate in the reaction (3,5). Future experiments will have to show whether the f-azidoalanine reacts directly with the DNA bases causing mispairing or whether further metabolic conversions (to, e.g., azidopyruvate) (6) are required for the interaction of the azide group with the DNA bases.…”

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confidence: 99%

“…To date, the nucleotide changes induced by sodium azide have not been investigated, although its high specificity has been noted in Salmonella and E. coli (3,4). Thus, only one missense his mutation (G-46) of Salmonella could be reverted…”

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confidence: 99%

“…Unlike other physical or chemical mutagens, it induces few, if any, chromosome aberrations (1)(2)(3). Up to 78% of chlorophylldeficient mutant seedlings per M1 spike progeny can be scored.…”

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confidence: 99%

“…Future experiments will have to show whether the f-azidoalanine reacts directly with the DNA bases causing mispairing or whether further metabolic conversions (to, e.g., azidopyruvate) (6) are required for the interaction of the azide group with the DNA bases. While sodium azide is a potent mutagen in several other higher plants, Escherichia coli, yeast, and the housefly, it fails to increase mutation frequencies in Arabidopsis, Drosophila, and Neurospora and is only weakly mutagenic in mammalian cells (3).…”

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Sodium azide mutagenesis: preferential generation of A.T-->G.C transitions in the barley Ant18 gene.

Olsen

Wang

Wettstein

1993

Proc. Natl. Acad. Sci. U.S.A.

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The molecular basis for the absence of anthocyanins and proanthocyanidins in four independent sodium azide-induced antl8 mutants of barley was examined by sequencing the gene encoding dihydroflavonol 4-reductase in these mutants. Sodium azide generated 21 base substitutions, which corresponds to 0.17% of the 12,704 nucleotides sequenced. Of the substitutions, 86% were nucleotide transitions, and 14% were transversions. AT -G-C base pair transitions were about 3 times more frequent than G-C -) AT transitions. No deletions or mutation hot spots were found. The absence of dihydroflavonol 4-reductase activity in antl8-159, antl8-162, and antl8-164 plants is caused by missense mutations in the respective genes. By using microprojectile bombardment, a plasmid harboring the wild-type Antl8 gene was introduced into antl8-161 mutant cells and resulted in the development of anthocyanin pigmentation, which demonstrates that the mutation is corrected by expression of the introduced gene. On the other hand, a plasmid derivative with the two antl8-161-specific base transitions at the 5' splice site of intron 3 prevented complementation. It is concluded that the absence of detectable mRNA for dihydroflavonol 4-reductase in antl8-161 cells is due to the mutations in the pre-mRNA splice donor site.

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Biochemical and mutagenic characterization of plant‐activated aromatic amines

Plewa

Gichner

Xia

et al. 1993

Enviro Toxic and Chemistry

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Plant activation is the process by which promutagenic agents are activated into rautagens by plant systems. With the widespread use of agricultural chemicals on crop plants and with the global exposure of plants to pollutants, the possibility exists that plant‐activated agents may be introduced into the human food chain. The plant cell/microbe coincubation assay uses cultured plant cell suspensions as the activating system and bacteria or yeast cells as the genetic indicator organism. After a treatment time, the microbes are plated on selective medium. In this way the activation system and the genetic system can be independently studied. In addition, the viability of the plant cells and the microbial cells can be independently determined so that the toxicity of a test agent can be evaluated. Using cytochrome P450 monooxygenase and peroxidase inhibitors, we are studying the biochemical mechanisms of plant activation of environmental contaminants, especially aromatic amines. We propose a working model for the activation of aromatic amines by cultured tobacco cells into stable frameshift mutagens.

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Metabolic activation of the mutagen azide in biological systems

Cited by 76 publications

References 62 publications

Mutagenic and antimutagenic properties of some lichen species grown in the Eastern Anatolia Region of Turkey

Mutagenic and antimutagenic properties of some lichen species grown in the Eastern Anatolia Region of Turkey

Sodium azide mutagenesis: preferential generation of A.T-->G.C transitions in the barley Ant18 gene.

Biochemical and mutagenic characterization of plant‐activated aromatic amines

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