Metabolic activity of subterranean microbial communities in deep granitic groundwater supplemented with methane and H2

Pedersen, Karsten

doi:10.1038/ismej.2012.144

Cited by 44 publications

(69 citation statements)

References 30 publications

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“…In a previous study, the production of at most 320 mM acetate was observed in FCCSs charged with ONK-PVA6 groundwater, and it was speculated that the acetate was produced by microorganisms using methane as the source of carbon (Pedersen 2013). Several of the SRB in ONK-PVA6 (Table 7) are known as complete acetate-oxidizing genera, i.e., Desulfobacterium (Brysch et al 1987), Desulfobacula, and Desulfotignum (Kuever et al 2001).…”

Section: Effect Of Sulphate On Srb Diversity and Activitymentioning

confidence: 96%

Sulphate-controlled Diversity of Subterranean Microbial Communities over Depth in Deep Groundwater with Opposing Gradients of Sulphate and Methane

Pedersen

Bengtsson²,

Edlund³

et al. 2014

Geomicrobiology Journal

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The groundwater system in Olkiluoto, Finland, is stratified with a mixing layer at a depth of approximately 300 m between sulphaterich, methane-poor and sulphate-poor, methane-rich groundwaters. New sequence library data obtained by 454 pyrotag sequencing of the v4v6 16S rDNA region indicated that sulphate-reducing bacteria (SRB) dominated the mixing layer while SRB could not be detected in the deep sulphate-poor groundwater samples. With the indispensable support of the sequence data, it could be demonstrated that sulphate was the only component needed to trigger a very large community transition in deep sulphate-poor, methane-rich groundwater from a non-sulphate-reducing community comprising Hydrogenophaga, Pseudomonas, Thiobacillus, Fusibacter, and Lutibacter to a sulphate-reducing community with Desulfobacula, Desulfovibrio, Desufobulbaceae, Desulfobacterium, Desulfosporosinus, and Desulfotignum. Experiments with biofilms and planktonic microorganisms in flow cells under in situ conditions confirmed that adding sulphate to the sulphate-poor groundwater generated growth of cultivable SRB and detectable SRB-related sequences. It was also found that the 16S rDNA diversity of the biofilms was conserved over 103 d and that there was great similarity in diversity between the microorganisms in the biofilms and in the flowing groundwater. This work demonstrates that the presence/absence of only one geochemical parameter, i.e., sulphate, in the groundwater significantly influenced the diversity of the investigated subterranean microbial community.

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Section: Effect Of Sulphate On Srb Diversity and Activitymentioning

confidence: 96%

Sulphate-controlled Diversity of Subterranean Microbial Communities over Depth in Deep Groundwater with Opposing Gradients of Sulphate and Methane

Pedersen

Bengtsson²,

Edlund³

et al. 2014

Geomicrobiology Journal

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“…The influence of viruses on microbial processes has been identified as an important factor to include in model studies. In particular, they seem to have an important mitigation effect on sulphide production by SRB (Eydal, 2009;Kyle, 2008a;Pedersen, 2013) Microbial biofilms was found to significantly influence the sorption of radionuclides on glass and rock surfaces (Anderson, et al, 2006a(Anderson, et al, , 2007. In addition, it has been demonstrated that microbial iron oxidising biofilms are strong sorbents for trace elements (Anderson, et al, 2006b;Anderson and Pedersen, 2003).…”

Section: High-level Waste Repositoriesmentioning

confidence: 98%

“…Sampling procedures have been developed and thoroughly tested, as have underground facilities for model studies (Nielsen, 2006;Pedersen, 2012aPedersen, , 2013. The first important parameter to analyse in repository environments is biomass.…”

Section: High-level Waste Repositoriesmentioning

confidence: 99%

“…Of particular interest is the H2 produced during the anaerobic corrosion of iron components in a repository, for example, the rock bolts installed to secure the tunnel from falling rocks. Such corrosion, evolving H2, may lead to the local production of large amounts of sulphide (Pedersen, 2012a(Pedersen, , 2012b(Pedersen, , 2013. In HLRW repositories with sulphate-rich groundwater there is a strong potential for microbial sulphate reduction to sulphide via two metabolic processes.…”

Section: High-level Waste Repositoriesmentioning

confidence: 99%

“…First, in addition to the naturally occurring H2 in groundwater, iron in water-filled deep underground repository construction is bound to corrode anaerobically with the concomitant production of H2 (Reardon, 1995), which may induce SRB growth and sulphide production. Second, in the case where sulphate-rich groundwater mixes with deep methane-rich groundwater SRB growth and activity can be induced (Pedersen, 2013). Regarding the anaerobic metabolism of methane, the situation is obscure.…”

Section: High-level Waste Repositoriesmentioning

confidence: 99%

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Analysis of mineralogical and deformation-induced transformations of Nizhnekansky Massif rocks to estimate their retention capacity in geological disposal and isolation of radioactive waste

Петров¹,

Poluektov²,

Hammer³

et al. 2015

gzh

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JT03354141Complete document available on OLIS in its original format This document and any map included herein are without prejudice to the status of or sovereignty over any territory, to the delimitation of international frontiers and boundaries and to the name of any territory, city or area. . Since then, there have been major developments in a number of national geological disposal programmes and significant experience has been obtained in preparing and reviewing cases for the operational and long-term safety of proposed and operating geological repositories. Especially, three national programmes are now, or will shortly be, at the stage of licence application for a deep geological repository for the disposal of spent nuclear fuel or high-level and other long-lived radioactive waste.Thus, the purpose of this Symposium, "The Safety Case for Deep Geological Disposal of Radioactive Waste: 2013 State of the Art", was to assess the practice, understanding and roles of the safety case, as applied internationally at all stages of repository development, including the interplay of technical, regulatory and societal issues, as they have developed since 2007.The Symposium was organised and hosted by the Nuclear Energy Agency (NEA) of the Organisation for Economic Co-operation and Development (OECD), and co-sponsored by the EC and the IAEA.The 2013 Symposium attracted 168 participants from 65 organisations and 17 countries and international bodies.The Symposium was chaired by Klaus-Jürgen Röhlig, who is also the Chair of the NEA Integration Group for the Safety Case (IGSC). The planning and implementation of the symposium were supported by the efforts of staff of the NEA and of the programme committee.This synopsis was drafted by Trevor Sumerling (Safety Assessment Management Ltd, UK), finalised under the direction of the Chair and Gloria Kwong of the NEA and approved by the programme committee. Deep Geological Repositories (1999). Since then the concept has been elaborated on in NEA documents describing the nature and purpose of safety cases , 2013a and has been the basis of NEA/IGSC collaborative projects and EU projects (EC, 2011). The concept has been taken up in international safety standards as promulgated by the IAEA (2006, 2011) and recently in ICRP recommendations on the application of the system of radiological protection to geological disposal (ICRP, 2013).Meanwhile, the concept has been developed practically and applied within many national radioactive waste disposal programmes and also taken up in some national regulatory guides. The NEA has used the concept as a guide in several international peer reviews of national repository programmes and safety documentation. In Europe, the EU Directive 2011/70/Euratom (EU, 2011) establishes a framework to ensure responsible and safe management of spent fuel and radioactive waste by member states that, inter alia, requires a decision-making process based on safety evidence and arguments that mirrors the safety case concept. Aims of the symposiumThe purpose of thi...

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Adenosine triphosphate (ATP) as a metric of microbial biomass in aquatic systems: new simplified protocols, laboratory validation, and a reflection on data from the literature

Bochdansky

Stouffer

Washington

2021

Limnology & Ocean Methods

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The use of adenosine triphosphate (ATP) as a universal biomass indicator is built on the premise that ATP concentration tracks biomass rather than the physiological condition of cells. However, reportedly high variability in ATP in response to environmental conditions is the main reason the method has not found widespread application. To test possible sources of this variability, we used the diatom Thalassiosira weissflogii as a model and manipulated its growth rate through nutrient limitation and through exposure to three different temperatures (15°C, 20°C, and 25°C). We simplified the ATP protocol with hot‐water or chemical extraction methods, modified a commercially available luciferin‐luciferase assay, and employed single‐photon counting in a scintillation counter, all of which increased sensitivity and throughput. Per‐cell ATP levels remained relatively constant despite changes in growth rates by approximately 10‐fold in the batch culture (i.e., nutrient limitation) experiments, and approximately 2‐fold in response to temperature. The re‐examination of related literature values revealed that average cellular ATP levels differed little among taxonomic groups of aquatic microbes, even at the domain level, and correlated well with bulk properties such as elemental carbon or nitrogen. Fulfilling multiple cellular functions in addition to being the universal energy currency requires ATP to be maintained in a millimolar concentration range. Consequently, ATP relates directly to live cytoplasm volume, while elemental carbon and nitrogen are constrained by an indeterminate pool of detrital material and intracellular storage compounds. The ATP‐biomass indicator is sensitive, economical, and can be readily standardized among laboratories and across environments.

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Metabolic activity of subterranean microbial communities in deep granitic groundwater supplemented with methane and H2

Cited by 44 publications

References 30 publications

Sulphate-controlled Diversity of Subterranean Microbial Communities over Depth in Deep Groundwater with Opposing Gradients of Sulphate and Methane

Sulphate-controlled Diversity of Subterranean Microbial Communities over Depth in Deep Groundwater with Opposing Gradients of Sulphate and Methane

Analysis of mineralogical and deformation-induced transformations of Nizhnekansky Massif rocks to estimate their retention capacity in geological disposal and isolation of radioactive waste

Adenosine triphosphate (ATP) as a metric of microbial biomass in aquatic systems: new simplified protocols, laboratory validation, and a reflection on data from the literature

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