Metabolic Alterations of Toxic and Nonessential Elements by the Treatment of Sempervivum tectorum Extract in a Hyperlipidemic Rat Model

Abstract

A hyperlipidemic rat model was used to examine the therapeutic effect of Sempervivum tectorum plant extract on the metabolic alterations of Al, As, B, Ba, Cd, Hg, Ni, Pb, and Ti in the liver and bile. Hyperlipidemia was produced by lipogenic diet and alcohol and verified by morphological investigation of the liver with the aid of light and an electron microscope. Element concentration in the liver and bile were determined by inductively coupled plasma optical emission spectrometry (ICP-OES). The concentration values in the liver higher than the detection limit (Al, Ba, Ni, Ti) were unambiguous. Significant differences were found for the four groups at p < 0.05 level (ANOVA). A significant difference was observed between Al and B concentration in the bile fluids of the 4 groups ( p < 0.05). The excretion of Al and Ti into the bile fluid increased significantly ( p < 0.05). Following the administration of S. tectorum extract to rats with hyperlipidemia, the excretion of Al, B and Ba increased, whereas the excretion of Ti decreased significantly ( p < 0.05). The favorable action of the extract (protecting the liver in hyperlipidemic rats) was verified by morphological studies, and its detoxicating property was shown by the elimination of Al, Ba, Ni, and Ti from the liver.

Protective Effects of Aqueous Extract of <i>Sempervivum tectorum</i> L (<i>Crassulaceae</i>) on Aluminium-Induced Oxidative Stress in Rat Blood

“…Administration of S. tectorum one month after AS administration was stopped had not the same effect on Al level, which was not significantly lower than in Al group and was significantly higher than in Al+St group. Our results were in accordance with Szentmihályi et al [31] which observed a significant reduction of Al level in liver of hyperlipidemic rats folowing S. tectorum extract administration due to elevation of Al excretion.…”

Cisplatin administration influences on toxic and non-essential element metabolism in rats

“…Inductively coupled plasma optical emission spectrometric (ICP-OES) method was applied for measuring Al, B, Ba, Cr, Li, Ni, Pb, Pt, Sb, Si, Sn, Sr and V content [11] using a Spectro Genesis ICP-OES (Kleve, Germany) equipment. For the standardization of equipment and measurements, Spectro multi-element and Spectrum 3D standards were used.…”

Sex-dependent alterations in erythrocyte trace element levels and antioxidant status after a month of moderate daily red wine consumption

“…Standard solutions in different concentrations (0, 1, 5, 10, 20, 50 mg/ml) were measured for calibration curves. As a matrix effect is present at ICP measurements, standards were prepared in the same matrix (in 5% HNO 3 solutions) as the samples [26,27].…”