Metabolic analysis of elicited cell suspension cultures of Cannabis sativa L. by 1H-NMR spectroscopy

Peč, Jaroslav; Flores-Sánchez, Isvett Josefina; Choi, Young Hae; Verpoorte, Robert

doi:10.1007/s10529-010-0225-9

Cited by 22 publications

(11 citation statements)

References 18 publications

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“…In hemp suspension cells, elicitation with biotic and abiotic elicitors did not induce an increase in cannabinoids ( Flores-Sanchez et al, 2009 ); however, jasmonic acid was shown to elicit the production of the antioxidant tyrosol ( Pec et al, 2010 ).…”

Section: Cannabis Biotechnology: Challenges and Prospectsmentioning

confidence: 99%

Cannabis sativa: The Plant of the Thousand and One Molecules

2016

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Cannabis sativa L. is an important herbaceous species originating from Central Asia, which has been used in folk medicine and as a source of textile fiber since the dawn of times. This fast-growing plant has recently seen a resurgence of interest because of its multi-purpose applications: it is indeed a treasure trove of phytochemicals and a rich source of both cellulosic and woody fibers. Equally highly interested in this plant are the pharmaceutical and construction sectors, since its metabolites show potent bioactivities on human health and its outer and inner stem tissues can be used to make bioplastics and concrete-like material, respectively. In this review, the rich spectrum of hemp phytochemicals is discussed by putting a special emphasis on molecules of industrial interest, including cannabinoids, terpenes and phenolic compounds, and their biosynthetic routes. Cannabinoids represent the most studied group of compounds, mainly due to their wide range of pharmaceutical effects in humans, including psychotropic activities. The therapeutic and commercial interests of some terpenes and phenolic compounds, and in particular stilbenoids and lignans, are also highlighted in view of the most recent literature data. Biotechnological avenues to enhance the production and bioactivity of hemp secondary metabolites are proposed by discussing the power of plant genetic engineering and tissue culture. In particular two systems are reviewed, i.e., cell suspension and hairy root cultures. Additionally, an entire section is devoted to hemp trichomes, in the light of their importance as phytochemical factories. Ultimately, prospects on the benefits linked to the use of the -omics technologies, such as metabolomics and transcriptomics to speed up the identification and the large-scale production of lead agents from bioengineered Cannabis cell culture, are presented.

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Section: Cannabis Biotechnology: Challenges and Prospectsmentioning

confidence: 99%

Cannabis sativa: The Plant of the Thousand and One Molecules

2016

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“…In this work, several compounds were tested on cell suspensions obtained from the industrial hemp variety Futura to identify the best elicitor treatment that is able to induce secondary metabolism and to elucidate the metabolic pathways activated by the elicitation. The elicitors were chosen based on results achieved both on cannabis cell cultures [18] and on other species ([19] and references therein). Namely, CHT, SA, JA, and MeJA were tested in this study, as well as a combination of elicitation and precursor feeding.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of tyrosol, an antioxidant compound mainly found in olive oil and in plants of the Rhodiola species, was described for the first time in cell suspensions of a drug-type hemp variety by Peč et al [18]. These authors obtained two types of cell lines, green and greenish-brown cells, that produced more tyrosol than olive oil after JA elicitation.…”

Section: Discussionmentioning

confidence: 99%

“…As far as cannabinoids are concerned, callus and cell suspension cultures are not able to produce these kinds of molecules probably because their synthesis is restricted to specialized tissues, the trichomes [16]. However, other interesting secondary metabolites are synthesized by different hemp plant tissues and could be obtained by plant cell cultures [15,17,18]. In a previous study on several hemp varieties, we reported the presence of flavonoid compounds from leaves, mainly apigenin- and luteolin-7-O-glucoside [7].…”

Section: Introductionmentioning

confidence: 99%

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Cell Suspensions of Cannabis sativa (var. Futura): Effect of Elicitation on Metabolite Content and Antioxidant Activity

et al. 2019

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Cannabis sativa L. is one of the most-studied species for its phytochemistry due to the abundance of secondary metabolites, including cannabinoids, terpenes and phenolic compounds. In the last decade, fiber-type hemp varieties have received interest for the production of many specialized secondary metabolites derived from the phenylpropanoid pathway. The interest in these molecules is due to their antioxidant activity. Since secondary metabolite synthesis occurs at a very low level in plants, the aim of this study was to develop a strategy to increase the production of such compounds and to elucidate the biochemical pathways involved. Therefore, cell suspensions of industrial hemp (C. sativa L. var. Futura) were produced, and an advantageous elicitation strategy (methyl jasmonate, MeJA) in combination with precursor feeding (tyrosine, Tyr) was developed. The activity and expression of phenylalanine ammonia-lyase (PAL) and tyrosine aminotransferase (TAT) increased upon treatment. Through 1H-NMR analyses, some aromatic compounds were identified, including, for the first time, 4-hydroxyphenylpyruvate (4-HPP) in addition to tyrosol. The 4-day MeJA+Tyr elicited samples showed a 51% increase in the in vitro assay (2,2-diphenyl-1-picrylhydrazyl, DPPH) radical scavenging activity relative to the control and a 80% increase in the cellular antioxidant activity estimated on an ex vivo model of human erythrocytes. Our results outline the active metabolic pathways and the antioxidant properties of hemp cell extracts under the effect of specific elicitors.

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“…This method had been successfully used to differentiate Cannabis cultivars [14]. Moreover, 1 H-NMR based-metabolomics had been applied to study the impact of elicitation in cell suspension cultures of Cannabis [15][16]. Our previous work also applied 1 H-NMR-based metabolomics that combined with real time PCR (RT-PCR) technique for studying cannabinoid biosynthesis of Cannabis cultivars over flowering season [17].…”

Section: ■ Introductionmentioning

confidence: 99%

Metabolic Changes in the Trichomes of Cannabis sativa var. bedrobinol Analyzed by 1H-NMR-Based Metabolomics

Happyana

Kayser

2020

Indones. J. Chem.

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Trichomes of Cannabis sativa are the main tissue for synthesizing and storing cannabinoids, the most interesting compounds in this plant. In this report, metabolic changes in the trichomes of C. sativa var. bedrobinol were investigated by 1H-NMR-based metabolomics over the flowering session. Three cannabinoids, including Δ9-tetrahydrocannabinolic acid (THCA), cannabichromenic acid (CBCA), and Δ9-tetrahydrocannabinol (THC), were successfully identified in the chloroform extracts of the Cannabis trichomes. Meanwhile, 20 non-cannabinoid compounds, including sugars, amino acids, and other acidic constituents, were detected in the water extracts. Metabolic changes of the Cannabis trichomes during the monitoring time were successfully revealed using the models of partial least squares discriminant analysis (PLSDA) and 1H-NMR quantitative analysis. Score plots of the PLSDA models classified metabolomes based on the harvest time. Discriminant metabolites for the differentiation were detected in the loading plots of the models. THCA was found as an important discriminant compound in the chloroform extracts, while all quantified water-soluble compounds were detected, contributing to the metabolic changes of the water extracts. The obtained results shed more light on the biosynthesis of metabolites in the Cannabis trichomes over the flowering season.

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Metabolic analysis of elicited cell suspension cultures of Cannabis sativa L. by 1H-NMR spectroscopy

Cited by 22 publications

References 18 publications

Cannabis sativa: The Plant of the Thousand and One Molecules

Cannabis sativa: The Plant of the Thousand and One Molecules

Cell Suspensions of Cannabis sativa (var. Futura): Effect of Elicitation on Metabolite Content and Antioxidant Activity

Metabolic Changes in the Trichomes of <i>Cannabis sativa</i> var. <i>bedrobinol</i> Analyzed by <sup>1</sup>H-NMR-Based Metabolomics

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