Metabolic analysis of four phenolic acids in rat by liquid chromatography–tandem mass spectrometry

Simultaneous determination of caffeic acid phenethyl ester and its metabolite caffeic acid in dog plasma using liquid chromatography tandem mass spectrometry

“…Moreover, a variety of methods has been established to identity or quantify CA in various matrices including plant tissues [38][39][40][41], rat plasma [42,43], human plasma [44,45] and human serum [46]. Those methods involved high performance liquid chromatography coupled with electrochemical detector [47][48][49], HPLC-UV [50], gas chromatography coupled with mass spectrometry [51], LC-MS [39,40] and LC-MS/MS [41,[43][44][45]. Nevertheless, no study has reported a method for the simultaneous determination of CAPE and CA in biological matrices.…”

Simultaneous determination of danshensu, rosmarinic acid, cryptotanshinone, tanshinone IIA, tanshinone I and dihydrotanshinone I by liquid chromatographic–mass spectrometry and the application to pharmacokinetics in rats

“…The in vivo simultaneous quantification analysis of these two kinds of active components from the roots of SMB has become more and more important due to the increasingly extensive application of danshen preparations in worldwide. The in vitro and in vivo quantitative methods of HPLC equipped with UV or MS in detecting either the water-soluble phenolic acids or the lipophilic tanshinone components have been reported [9][10][11][12][13][14][15][16], However, until now, there are very few reports on the in vivo simultaneous detection of the two active components with HPLC-UV methods and the quantitative analyses of these components are very limited [17,18]. Due to the significant differences on the chemical structures and the pharmacological activities between the two groups of the active components, it is very necessary to develop a more efficient method for in vivo simultaneous detection of the two groups of the active components which are as the main active biomarkers in TCM compound preparations containing danshen extracts.…”

Characterization of metabolites in rat plasma after intravenous administration of salvianolic acid A by liquid chromatography/time‐of‐flight mass spectrometry and liquid chromatography/ion trap mass spectrometry

“…The initial identification of the metabolites is discussed as follows. The mass ( m/z 669.1452) of the protonated molecule of M1 was 176 mass units higher than that of SalA ( m/z 493.1134), and glucuronidation is a very common metabolic pathway of drug in vivo ,12–14 suggesting that it might be a conjugate of SalA with a glucuronic acid, namely SalA‐monoglucuronide. The mass ( m/z 683.1611) of the protonated molecule of M2 was 14 mass units higher than that of M1, and the action of catechol‐3‐O‐methyltransferase (COMT) in the biosystem, suggesting that it might be a methylated form of M1, namely monomethyl‐SalA‐monoglucuronide.…”