Metabolic and biological profile of autochthonous Vitis vinifera L. ecotypes

Abstract

Vitis vinifera L. is a plant species rich in phenolic compounds that are usually associated with the health benefits of wine and grape consumption in the diet. Anthocyanins, catechins, flavonol, phenolic acids and stilbenes are key molecular constituents of the Vitis berries, affecting the quality of grape products. The purpose of this work was to identify the metabolic profiles of 37 genetically certified V. vinifera Latial accessions. In particular, qualitative and quantitative analyses of specific secondary metabolites and total phenolic and tannin contents were performed by LC-MS and spectrophotometric analysis. In addition, since plant molecules are well-known for their free radical scavenging properties, the antioxidant effects of the sample extracts were evaluated through two different antiradical assays: DPPH and FRAP tests. Finally, a preliminary screening of the antiproliferative activity of each specimen on HCT-116 human colorectal cancer cells was conducted. All the results showed a great variety and amount of phenolic compounds in all accessions; moreover, we observed a significant correlation in the extracts between the metabolite concentration and bioactivity. Besides, some samples presented extraordinary biological effects, such as reduction of tumor cell growth not associated with cytotoxicity, supporting their use as possible future adjuvants for cancer therapy. In conclusion, the present research increased the scientific knowledge about Italian autochthonous vine ecotypes in order to valorize them and support their reintroduction in the local economic system.

From Robinia pseudoacacia L. nectar to Acacia monofloral honey: biochemical changes and variation of biological properties

“…Total simple phenolic levels were estimated using the Folin–Ciocalteu spectrophotometric method, modified as described by Impei et al . A standard curve (0–30 mg L −1 ; R 2 = 0.994) with pure gallic acid (GA) was calculated to extrapolate the phenolic concentration in each specimen.…”

Inhibitory Effect of Dried Pomegranate Concentration Powder on Melanogenesis in B16F10 Melanoma Cells; Involvement of p38 and PKA Signaling Pathways

“…DPPH radical scavenging assay was performed according to the method of Impei et al [ 66 ]. Briefly, 0.2 mM DPPH solution (Sigma-Aldrich) in methanol was prepared and used immediately.…”

Luteolin induces apoptotic cell death via antioxidant activity in human colon cancer cells

“…Flavonoids are biologically active polyphenolic compounds widely distributed in plants, and luteolin, 3',4',5,7-tetrahydroxyflavone, is rich in vegetables and flowers. Flavonoids including luteolin have been focused on anticancer properties via various anticancer mechanisms (10)(11)(12)(13). Luteolin have antiinflammatory, antiallergic, anticancer, antioxidant and ROS scavenging activities (14)(15)(16).…”