Metabolic and digestive enzyme activity of<i>Pangasianodon hypophthalmus</i>(Sauvage, 1878) fingerlings in response to alternate feeding of different protein levels in the diet

Tok, Nagung Camder; Jain, Kartik; Prabu, D Linga; Sahu, Narottam Prasad; Munilkumar, S.; Pal, A. K.; Siddiah, Gunnur M; Kumar, Pankaj

doi:10.1111/are.13122

Cited by 21 publications

(6 citation statements)

References 71 publications

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“…However, the discrimination of SOD activity in this study may be due to the different life stages of L. vannamei . AKP can catalyse the hydrolysis of phosphate monoesters and transfer phosphate groups, which is of great significance to animal survival (Tok et al, ). This experiment showed the appropriate level of dietary n‐3HUFA supplementation enhanced AKP activity both in subadult and adult L. vannamei ; however, further increasing the level of n‐3HUFA caused a decrease.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary n‐3HUFA on different growth stages of the white shrimp,Litopenaeus vannamei: Growth, haematological characteristics, enzyme activities and fatty acid profiles

et al. 2019

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This study investigated the effects of n-3 high unsaturated fatty acid (n-3HUFA) levels on the growth performance, antioxidant enzyme activities and fatty acid profiles of both subadult and adult Litopenaeus vannamei (L. vannamei). Seven iso-nitrogenous and iso-lipidic diets were used, containing n-3HUFA concentrations of 1.6 (control), 4.8, 7.4, 13.9, 23.9, 29.2 and 34.4 g/kg, respectively. Two 8-week feeding trials were conducted to determine the dietary n-3HUFA requirements of L. vannamei with an initial body weight of 4.25 ± 0.00 g (subadults) and 8.50 ± 0.01 g (adults). The results showed that the dietary n-3HUFA level significantly affected the weight gain rate (WGR), specific growth rate, the feed conversion ratio and the hepatosomatic index (HSI) (p < 0.05), but did not significantly affect the survival rate (p > 0.05). At appropriate level, dietary n-3HUFA improved growth performance and HSI of both subadult and adult L. vannamei. Both subadults and adults showed significant differences in body composition (p < 0.05), except for moisture and crude ash (p > 0.05). Cholesterol and low-density lipoprotein significantly decreased with increasing dietary n-3HUFA both in subadults and adults (p < 0.05); however, triglyceride showed no significant change (p > 0.05). High-density lipoprotein (HDL) in subadults was significantly affected by dietary n-3HUFA (p < 0.05), but followed no apparent regularity; HDL significantly changed in adults and showed an upward trend followed by a downward trend (p < 0.05). There was no significant effect on aspartate transaminase (AST) activity in subadults, but AST in adults and alanine transaminase (ALT) in subadults and adults were significantly affected (p < 0.05). Dietary n-3HUFA significantly affected serum polyphend oxidase, malic dehydrogenase, alkaline phosphatase, superoxide dismutase and sodium-potassium adenosine triphosphatase enzyme activities in gills

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Section: Discussionmentioning

confidence: 99%

Effects of dietary n‐3HUFA on different growth stages of the white shrimp,Litopenaeus vannamei: Growth, haematological characteristics, enzyme activities and fatty acid profiles

et al. 2019

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“…Protein:energy ratio is the main key to optimize fish growth and not let feed be converted into body fat. Protein, the most expensive feed ingredient, is necessary for metabolism and growth directly affecting fish performance (Tok et al., ). Lipids are an energy source for growth and metabolism, but when included in the wrong proportion to protein, they can affect feed consumption and body fat (Cho et al., ).…”

Section: Discussionmentioning

confidence: 99%

Dietary protein:lipid ratio changes growth, digestive enzyme activity, metabolic profile and haematological parameters in hybrid surubim (Pseudoplatystoma fasciatum × Leiarius marmoratus)

et al. 2018

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Studies investigating improved protein:lipid ratios based on their effects on growth, nutrient utilization, digestive enzyme activities, blood metabolites and erythogram in tropical carnivorous fish are very scarce. This study evaluated the effect of different protein:lipid ratios on these parameters in the hybrid surubim Pseudoplatystoma corruscans × Leiarius marmoratus. Juvenile fish (8.90 ± 0.94 g initial weight) were fed 3% of total biomass for 60 days using four isoenergetic experimental diets with different protein:lipid ratios (9.00, 4.60, 3.54 and 1.78). Higher growth performance parameters were observed at higher protein:lipid ratios. The activities of intestine total alkaline protease, trypsin and lipase (U/mg protein) were not affected by dietary treatments. Chymotrypsin (U/mg protein) was higher in the 4.60 protein:lipid ratio group. Amylase (U/mg protein) was higher in intermediate groups. Blood glucose, total plasma protein, triglycerides, cholesterol and free amino acids decreased as the protein:lipid ratio decreased. Haematocrit was higher at the 9.00 and 4.60 protein:lipid ratios. Hybrid surubim showed metabolic adaptation to the different protein:lipid ratios tested. These results suggest that the 4.60 protein:lipid ratio showed the best protein‐sparing effect of lipids.

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“…In the conditions of decrease in live weight of carp infected by B. acheilognathi, the deposition of lipids and reserve proteins in the carp tissues significantly reduces, negatively affecting their need for metabolic energy in winter, and thus -their survival (Rehulka et al, 2004;Yuskiv, 2006;Yuskiv & Yuskiv, 2020). According to some authors (Nanware et al, 2012), the survival of carp largely depends on their total body weight, which is affected the most by the level and quality of feeding and diseases (Rehulka & Minařík, 2007;Tok et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

The synthesis of lipids and proteins in vitro in tissues of Cyprinus carpio infected with Bothriocephalus acheilognathi

Yuskiv

2021

Regul. Mech. Biosyst.

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The problem of the mechanisms of regulation of biochemical processes in carp Cyprinus carpio (Linnaeus, 1758) tissues and organs caused by infection with Bothriocephalus (Schyzocotyle) acheilognathi (Yamaguti, 1934) at different intensities of invasion remains practically unstudied. The purpose of this study was to dedetrmine the intensity of lipid and protein synthesis in vitro when [6-14C]glucose and [2-14C]lysine are used as their precursor in the tissues of the intestine, hepatopancreas and skeletal muscles of carp. The study was conducted on this-year carp with body weight 14.5–20.5 g, at different invasion rates of the helminth B. acheilognathi, which belongs to the family Bothriocephalidae of the Pseudophyllidae order of the Cestoda class of the Plathelminthes phylum. The examined carp were divided into three groups: 1st group of fish was free from intestinal helminths of B. acheilognathi (control); 2nd group of fish was weakly infected with helminths (intensity of invasion was 1–3 helminths per fish); the 3rd group of fish was highly infected (the invasion intensity was 4 worms and more per fish). Our results showed that in fish infected with the helminth B. acheilognathi in comparison to uninfected, the intensity of lipid synthesis in the intestinal wall, hepatopancreas, skeletal muscle was much lower when [6-14C]glucose was used as a predecessor than when [2-14C]lysine was used as a predecessor. In the examined tissues, significant decrease was observed in the synthesis of reserve lipids (mono-, di- and triacylglycerols) in comparison to the structural (phospholipids and cholesterol), which depends on the intensity of the B. acheilognathi invasion. In the metabolic processes in the wall of the intestine, hepatopancreas, skeletal muscle of this-year carp infectd with B. acheilognathi helminths, under in vitro conditions, [6-14C]glucose was used more than [2-14C]lysine. The intensity of protein synthesis in the intestinal wall, hepatopancreas, skeletal muscles of this-year carp infected with the helminth B. acheilognathi under in vitro conditions increased when [6-14C]glucose was added to the incubation medium, on average 7.1–28.3% and decreased when [2-14C]lysine was added, on average 7.8–25.7%.

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Metabolic and digestive enzyme activity ofPangasianodon hypophthalmus(Sauvage, 1878) fingerlings in response to alternate feeding of different protein levels in the diet

Cited by 21 publications

References 71 publications

Effects of dietary n‐3HUFA on different growth stages of the white shrimp,Litopenaeus vannamei: Growth, haematological characteristics, enzyme activities and fatty acid profiles

Effects of dietary n‐3HUFA on different growth stages of the white shrimp,Litopenaeus vannamei: Growth, haematological characteristics, enzyme activities and fatty acid profiles

Dietary protein:lipid ratio changes growth, digestive enzyme activity, metabolic profile and haematological parameters in hybrid surubim (Pseudoplatystoma fasciatum × Leiarius marmoratus)

The synthesis of lipids and proteins in vitro in tissues of Cyprinus carpio infected with Bothriocephalus acheilognathi

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