Metabolic and endocrine profiles and reproductive parameters in dairy cows under grazing conditions: effect of polymorphisms in somatotropic axis genes

Abstract

BackgroundThe present study hypothesized that GH-AluI and IGF-I-SnabI polymorphisms do change the metabolic/endocrine profiles in Holstein cows during the transition period, which in turn are associated with productive and reproductive parameters.MethodsHolstein cows (Farm 1, primiparous cows, n = 110, and Farm 2, multiparous cows, n = 76) under grazing conditions were selected and GH and IGF-I genotypes were determined. Blood samples for metabolic/endocrine determinations were taken during the transition period and early lactation in both farms. Data was analyzed by farm using a repeated measures analyses including GH and IGF-I genotypes, days and interactions as fixed effects, sire and cow as random effects and calving date as covariate.Results and DiscussionFrequencies of GH and IGF-I alleles were L:0.84, V:0.16 and A:0.60, B:0.40, respectively. The GH genotype was not associated with productive or reproductive variables, but interaction with days affected FCM yield in multiparous (farm 2) cows (LL yielded more than LV cows) in early lactation. The GH genotype affected NEFA and IGF-I concentrations in farm 1 (LV had higher NEFA and lower IGF-I than LL cows) suggesting a better energy status of LL cows.There was no effect of IGF-I genotype on productive variables, but a trend was found for FCM in farm 2 (AB cows yielded more than AA cows). IGF-I genotype affected calving first service interval in farm 1, and the interaction with days tended to affect FCM yield (AB cows had a shorter interval and yielded more FCM than BB cows). IGF-I genotype affected BHB, NEFA, and insulin concentrations in farm 1: primiparous BB cows had lower NEFA and BHB and higher insulin concentrations. In farm 2, there was no effect of IGF-I genotype, but there was an interaction with days on IGF-I concentration, suggesting a greater uncoupling somatropic axis in AB and BB than AA cows, being in accordance with greater FCM yield in AB cows.ConclusionThe GH and IGF-I genotypes had no substantial effect on productive parameters, although IGF-I genotype affected calving-first service interval in primiparous cows. Besides, these genotypes may modify the endocrine/metabolic profiles of the transition dairy cow under grazing conditions.

A polymorphism in the insulin-like growth factor 1 gene is associated with postpartum resumption of ovarian cyclicity in Holstein-Friesian cows under grazing conditions

“…In the current study, allele frequencies of the SNP IGF-1 / Sna BI estimated for the UHFxNZHF group (A: 0.52, B: 0.48) as well as for the total sample (A: 0.59, B: 0.41), are in agreement with those previously published in North American (NA) [24], Polish [25], and Uruguayan [29] HF populations (A: 0.55, 0.52 and 0.60; B: 0.45, 0.48 and 0.40, respectively). On the other hand, allele frequencies in the sample of UHF cows tended to present a different distribution (A: 0.63, B: 0.37), which is similar to that (A: 0.64, B: 0.36) reported by Ge et al [23] in Angus beef cattle.…”

“…In addition, Siadkowska et al [25] and Bonakdar et al [26] reported associations between the AB genotype and higher percentage of milk fat and protein in Polish and Iranian HF cows, respectively. Likewise, previous studies from our group have shown that AB cows tended to yield more FCM than primiparous BB cows at early and middle lactation and than multiparous AA cows over the lactation period [29]. No other reports were found in the literature concerning the effect of the SNP IGF-1 / Sna BI on milk production traits.…”

“…In dairy cattle, some studies have analyzed the association of this SNP with milk production traits [24-29]. However, studies evaluating the effect of this SNP on fertility traits in dairy cows are even more recent and scarce [28-30], with only the work from Ruprechter et al [29] reporting a significant effect of the SNP IGF-1 / Sna BI on CFS interval in primiparous Holstein-Friesian (HF) cows. To our knowledge there is no other published information showing a significant effect of this SNP on reproductive performance either in beef or dairy cattle.…”

The effect of polymorphism in gene of insulin-like growth factor-I on the serum periparturient concentration in Holstein dairy cows

“…Postpartum peripheral IGF-I levels are affected by breed/genotype [16] . Different genotypes can alter the endocrine and metabolic profiles of the transition dairy cow under grazing condition [17] . Several studies reported periparturient changes of IGF-I blood concentration in cattle [18,19] .…”

“…Finally, the genomic DNAs were eluted in a volume of 100 毺L supplied elution buffer. The extracted DNA was diluted to a working concentration of [10][11][12][13][14][15][16][17][18][19][20] ng/毺L, and 2.4 毺L of it was used as a template in PCR.…”