Metabolic and oxidative stress markers in Wistar rats after 2 months on a high-fat diet

Auberval, N.; Dal, Stéphanie; Bietiger, W.; Pinget, M.; Jeandidier, N.; Maillard-Pedracini, Elisa; Schini‐Kerth, Valérie B.; Sigrist, S.

doi:10.1186/1758-5996-6-130

Cited by 60 publications

(60 citation statements)

References 48 publications

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“…Interestingly, the increase in liver triacylglycerol concentrations occurred independently of any changes in fasting plasma triacylglycerol, as plasma levels were previously found to be normal (Sweazea et al, ). These findings are in contrast with studies that administered adult rats a mixed high‐carbohydrate and HFD for longer durations (Auberval et al, ).…”

Section: Discussioncontrasting

confidence: 88%

Six‐Week High‐Fat Diet Alters the Gut Microbiome and Promotes Cecal Inflammation, Endotoxin Production, and Simple Steatosis without Obesity in Male Rats

Crawford

Whisner

Al‐Nakkash

et al. 2019

Lipids

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Energy‐dense foods can alter gut microbial diversity. However, the physiological effects of diet‐induced microbial changes on the development of nonalcoholic fatty liver disease (NAFLD) remain debatable. We hypothesized that high‐fat intake for 6 weeks would promote intestinal dysbiosis by increasing gram‐positive bacteria, inducing the intestinal production of proinflammatory cytokines and subsequent hepatic lipid infiltration in young male rats. Six‐week old male Sprague–Dawley rats were divided into two groups and fed either a standard rodent chow or a 60% high‐fat diet (HFD) for 6 weeks. Chromogenic endotoxin quantification assays indicate an increase in lipopolysaccharide concentration in the plasma of HFD rats (p = 0.032). Additionally, Western blot analyses of the cecum showed significantly greater protein expression of the transcription factor, nuclear factor kappa B (NF‐kB), (p = 0.037) and the proinflammatory cytokine, interleukin‐1β (IL‐1β), (p = 0.042) in rats fed HFD. Linear discriminate analysis of effect size (LEfSe) showed greater abundance of Firmicutes and Actinobacteria in the samples collected from the cecum of HFD rats compared to chow. Consistent with the development of steatosis, the Oil‐Red‐O‐stained area was increased in liver sections from HFD rats. Hepatic triacylglycerol concentrations (p < 0.001) and plasma alanine aminotransferase (p < 0.001) were significantly increased in HFD‐fed animals compared to chow. These findings show that a short duration of high‐fat consumption can have profound deleterious effects on gastrointestinal health and the inflammatory state of these young male Sprague–Dawley rats.

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Section: Discussioncontrasting

confidence: 88%

Six‐Week High‐Fat Diet Alters the Gut Microbiome and Promotes Cecal Inflammation, Endotoxin Production, and Simple Steatosis without Obesity in Male Rats

Crawford

Whisner

Al‐Nakkash

et al. 2019

Lipids

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“…Oxidative stress in rats due to high fat diet feeding also increases the glucose intolerance and insulin resistance [42, 43]. Our investigation also revealed that high fat diet increased plasma and tissues level of oxidative stress markers.…”

Section: Discussionsupporting

confidence: 55%

Supplementation of Syzygium cumini seed powder prevented obesity, glucose intolerance, hyperlipidemia and oxidative stress in high carbohydrate high fat diet induced obese rats

Ulla

Alam

Sikder

et al. 2017

BMC Complement Altern Med

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BackgroundObesity and related complications have now became epidemic both in developed and developing countries. Cafeteria type diet mainly composed of high fat high carbohydrate components which plays a significant role in the development of obesity and metabolic syndrome.This study investigated the effect of Syzygium cumini seed powder on fat accumulation and dyslipidemia in high carbohydrate high fat diet (HCHF) induced obese rats.MethodMale Wistar rats were fed with HCHF diet ad libitum, and the rats on HCHF diet were supplemented with Syzygium cumini seed powder for 56 days (2.5% w/w of diet). Oral glucose tolerance test, lipid parameters, liver marker enzymes (AST, ALT and ALP) and lipid peroxidation products were analyzed at the end of 56 days. Moreover, antioxidant enzyme activities were also measured in all groups of rats.ResultsSupplementation with Syzygium cumini seed powder significantly reduced body weight gain, white adipose tissue (WAT) weights, blood glucose, serum insulin, and plasma lipids such as total cholesterol, triglyceride, LDL and HDL concentration. Syzygium cumini seed powder supplementation in HCHF rats improved serum aspartate amino transferase (AST), alanine amino transferase (ALT), and alkaline phosphatase (ALP) activities. Syzygium cumini seed powder supplementation also reduced the hepatic thiobarbituric acid reactive substances (TBARS) and elevated the antioxidant enzyme superoxide dismutase (SOD) and catalase (CAT) activities as well as increased glutathione (GSH) concentration. In addition, histological assessment showed that Syzygium cumini seed powder supplementation prevented inflammatory cell infiltration; fatty droplet deposition and fibrosis in liver of HCHFD fed rats.ConclusionOur investigation suggests that Syzygium cumini seed powder supplementation prevents oxidative stress and showed anti-inflammatory and antifibrotic activity in liver of HCHF diet fed rats. In addition, Syzygium cumini seed powder may be beneficial in ameliorating insulin resistance and dyslipidemia probably by increasing lipid metabolism in liver of HCHF diet fed rats.

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“…The importance of treatment intervals on tissue and plasma oxidative stress levels was emphasized in several studies. Auberval et al . demonstrated that although short‐term high‐fat diet (HFD) exhibits oxidative stress on pancreatic and hepatic tissues, this effect was not valid for plasma oxidative stress markers.…”

Section: Discussionmentioning

confidence: 99%

Adalimumab mitigates ovarian ischemia–reperfusion injury in rats by regulating oxidative stress, apoptosis and resolution of inflammation

Beyazıt

Bűyűk

Türkön

et al. 2018

J of Obstet and Gynaecol

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Aim: Ovarian torsion is a rare but an important reason of acute lower abdominal pain in women and associated with serious morbidity and mortality, if not treated promptly. The aim of this study was to evaluate the effects of an antitumor necrosis factor-α antibody on ovarian torsion in a rat model of ischemiareperfusion (I/R) injury. Methods: Forty female Wistar Albino rats were used in the present study. The rats were randomly divided into four groups: group I (sham), group II (I/R), group III (I/R + isotonic saline) and group IV (I/R + adalimumab). The I/R model was induced by torsion of both ovaries. Immunohistochemical staining for interleukin-1β (IL-1β), nuclear factor-κB (NF-κB), and inducible nitric oxide synthase was performed. Tissue and serum oxidative stress markers in conjunction with apoptotic index (AI) with the terminal deoxynucleotidyl transferase dUTP nick end labeling method were also calculated. Results: Tissue total oxidant status, oxidative stress index and nitric oxide values were significantly decreased, and tissue total antioxidant status was found to be increased in group IV. Inflammation, vascular congestion and hemorrhagia were significantly lower in adalimumab-treated group. Serum oxidative stress markers and tissue malondialdehyde levels did not differ in study groups. The AI was significantly increased in groups 2 and 3. Adalimumab treatment significantly decreased the AI. Conclusion: Adalimumab therapy in rats attenuated I/R induced ovarian injury, possibly suppressing inflammation, inhibiting oxidative stress, and altering apoptotic pathways.

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Metabolic and oxidative stress markers in Wistar rats after 2 months on a high-fat diet

Cited by 60 publications

References 48 publications

Six‐Week High‐Fat Diet Alters the Gut Microbiome and Promotes Cecal Inflammation, Endotoxin Production, and Simple Steatosis without Obesity in Male Rats

Six‐Week High‐Fat Diet Alters the Gut Microbiome and Promotes Cecal Inflammation, Endotoxin Production, and Simple Steatosis without Obesity in Male Rats

Supplementation of Syzygium cumini seed powder prevented obesity, glucose intolerance, hyperlipidemia and oxidative stress in high carbohydrate high fat diet induced obese rats

Adalimumab mitigates ovarian ischemia–reperfusion injury in rats by regulating oxidative stress, apoptosis and resolution of inflammation

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