Metabolic and physiological responses to progressive drought stress in bread wheat

Itam, Michael; Mega, Ryosuke; Tadano, Shota; Abdelrahman, Mostafa; Sachiko, Matsunaga; Yamasaki, Yuji; Akashi, Kinya; Tsujimoto, Hisashi

doi:10.1038/s41598-020-74303-6

Cited by 75 publications

(48 citation statements)

References 61 publications

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“…Another widely recognized mechanism of plants to minimize stress-induced oxidative damage to cells and cellular components is the alteration in the levels of various metabolites which finally shape up different metabolic pathways [ 39 , 40 ]. Since direct exposure to UV-B radiation could lower the antioxidant capacity of plants [ 41 ], consequently, metabolic alterations could compromise the plant defense system.…”

Section: Discussionmentioning

confidence: 99%

β-Sitosterol differentially regulates key metabolites for growth improvement and stress tolerance in rice plants during prolonged UV-B stress

Shahzad

Ewas

Harlina

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Elevated ultraviolet-B (UV-B) radiation is potentially deleterious to many organisms specifically crop plants and has become a global challenge. Rice is an exceptionally important staple food which is grown worldwide, and many efforts have been done recently to improve rice varieties against UV-B stress. This current study aims to investigate the effects of exogenous application of β-sitosterol (βSito) on growth improvement and tolerance level of rice plants against prolonged UV-B stress. The physiological and metabolic responses were evaluated in rice plants not supplemented with βSito (Nβ) and those supplemented with βSito (Sβ). Results The Nβ and Sβ plants were grown under non-stress (ns) and under prolonged UV-B stress (uvs) conditions and termed as Nβns, Sβns and Nβuvs, Sβuvs, respectively. The application of βSito contributes positively under non-stress and specifically to UV-B stress in terms of improving numerous physiological parameters associated with growth and development such as shoot and root length, RWC, whole plant biomass, chlorophyll pigments, and photosynthetic-related parameters (Pn, Gs, Tr, WUEi, Fv/Fm, and NPQ) in Sβ compared with Nβ plants. Moreover, enhanced oxidative stress tolerance of Sβuvs vs. Nβuvs plants under stress was attributed to low levels of ROS and substantial trigger in activities of antioxidant enzymes (SOD, POD, CAT, and APX). Metabolic analysis was performed using GC-TOFMS, which revealed higher accumulation of several key metabolites including organic acids, sugars, amino acids, and others in Sβuvs vs. Nβuvs plants, which were mainly reduced in Nβ plants under stress vs. non-stress conditions. Conclusion These results provide useful data regarding the important role of βSito on growth maintenance and modulation of several metabolites associated with osmotic and redox adjustments during UV-B stress tolerance in rice plants. Importantly, βSito-regulated plasticity could further be explored specifically in relation to different environmental stresses in other economically useful crop plants.

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Section: Discussionmentioning

confidence: 99%

β-Sitosterol differentially regulates key metabolites for growth improvement and stress tolerance in rice plants during prolonged UV-B stress

Shahzad

Ewas

Harlina

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…The aspartate family, comprising aspartic acid and asparagine, plays a crucial role in plants against drought stress. Accumulation of aspartate family, notably asparagine in plants during water deficit, has been reported [72]. The authors reported that the concentration of asparagine increased throughout the drought period from 4 to 10 days in bread wheat.…”

Section: Organic Acids Metabolic Changes To Drought Stressmentioning

confidence: 97%

Metabolomics Response to Drought Stress in Morus alba L. Variety Yu-711

Ackah

Shi

et al. 2021

Plants

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Mulberry is an economically significant crop for the sericulture industry worldwide. Stresses such as drought exposure have a significant influence on plant survival. Because metabolome directly reflects plant physiological condition, performing a global metabolomic analysis is one technique to examine this influence. Using a liquid chromatography-mass spectrometry (LC-MS) technique based on an untargeted metabolomic approach, the effect of drought stress on mulberry Yu-711 metabolic balance was examined. For this objective, Yu-711 leaves were subjected to two weeks of drought stress treatment and control without drought stress. Numerous differentially accumulated metabolic components in response to drought stress treatment were revealed by multivariate and univariate statistical analysis. Drought stress treatment (EG) revealed a more differentiated metabolite response than the control (CK). We found that the levels of total lipids, galactolipids, and phospholipids (PC, PA, PE) were significantly altered, producing 48% of the total differentially expressed metabolites. Fatty acyls components were the most abundant lipids expressed and decreased considerably by 73.6%. On the other hand, the prenol lipids class of lipids increased in drought leaves. Other classes of metabolites, including polyphenols (flavonoids and cinnamic acid), organic acid (amino acids), carbohydrates, benzenoids, and organoheterocyclic, had a dynamic trend in response to the drought stress. However, their levels under drought stress decreased significantly compared to the control. These findings give an overview for the understanding of global plant metabolic changes in defense mechanisms by revealing the mulberry plant metabolic profile through differentially accumulated compounds.

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“…Aspartate family, comprising aspartic acid and asparagine, plays a crucial role in plants against drought stress. Accumulation of aspartate family, notably asparagine in plants during water deficit, has been reported [77]. The authors reported that the concentration of asparagine increased throughout the drought period from 4 to 10 days in bread wheat.…”

Section: Organic Acids Metabolic Changes To Drought Stressmentioning

confidence: 97%

Metabolomics Response to Drought Stress in Morus alba L. variety Yu-711

Ackah¹,

Shi²,

Wu³

et al. 2021

Preprint

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Mulberry is an economically significant crop for the sericulture industry worldwide. Stresses such as drought exposure have a significant influence on plant survival. Metabolome directly reflects plant physiological status; thus, a way to assess this impact is to perform a global metabolomic analysis. This study investigated the effect of drought stress on mulberry Yu-711 metabolic balance using a liquid chromatography-mass spectrometry (LC-MS) based on an untargeted metabolomic approach. For this objective, Yu-711 leaves were subjected to two weeks of drought stress treatment and control without drought stress. Multivariate and univariate statistical analyses highlighted numerous differentially-accumulated metabolic elements as a function of time and treatment. Drought stress led to a more differentiated metabolites response than the control. We found that the levels of total lipids and galactolipids, and phospholipids (PC, PA, PE) were significantly altered, producing 48% of the total differentially expressed metabolites. Fatty acyls were the most abundant lipids expressed and decreased considerably by 73.6%. Prenol lipids class of lipids increased in drought leaves. Other classes of metabolites, including polyphenols( flavonoids and cinnamic acid), organic acid (amino acids), carbohydrates, benzenoids, and organoheterocyclic, all had a dynamic trend in response to the drought stress. However, their levels under drought stress generally decreased significantly compared to the control. These results provide an overview of the metabolic profile of the mulberry plant through differentially-accumulated compounds and provide a better understanding of global plant metabolic changes in defense mechanisms.

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Metabolic and physiological responses to progressive drought stress in bread wheat

Cited by 75 publications

References 61 publications

β-Sitosterol differentially regulates key metabolites for growth improvement and stress tolerance in rice plants during prolonged UV-B stress

β-Sitosterol differentially regulates key metabolites for growth improvement and stress tolerance in rice plants during prolonged UV-B stress

Metabolomics Response to Drought Stress in Morus alba L. Variety Yu-711

Metabolomics Response to Drought Stress in Morus alba L. variety Yu-711

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