Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish

Abstract

Obesity is a worldwide public health problem with increasing prevalence and affects 80% of diabetes mellitus type 2 cases. Zebrafish (Danio rerio) is an established model organism for studying obesity and diabetes including diabetic microvascular complications. We aimed to determine whether physical activity is an appropriate tool to examine training effects in zebrafish and to analyse metabolic and transcriptional processes in trained zebrafish. A 2- and 8-week experimental training phase protocol with adult zebrafish in a swim tunnel system was established. We examined zebrafish basic characteristics before and after training such as body weight, body length and maximum speed and considered overfeeding as an additional parameter in the 8-weeks training protocol. Ultimately, the effects of training and overfeeding on blood glucose, muscle core metabolism and liver gene expression using RNA-Seq were investigated. Zebrafish maximum speed was correlated with body length and was significantly increased after 2 weeks of training. Maximum swim speed further increased after 8 weeks of training in both the normal-fed and the overfed groups, but training was found not to be sufficient in preventing weight gain in overfed fish. Metabolome and transcriptome profiling in trained fish exhibited increased blood glucose levels in the short-term and upregulated energy supply pathways as well as response to oxidative stress in the long-term. In conclusion, swim training is a valuable tool to study the effects of physical activity in zebrafish, which is accompanied by metabolic and transcriptional adaptations.

The importance of mechanosensitive cell mediated prostaglandin and nitric oxide synthesis in the pathogenesis of apical periodontitis: comparative with chronic periodontitis

“…This means that inflammatory conditions can increase the formation of reactive oxygen species (ROS), which disrupt the redox balance in the body and cause oxidative damage. Therefore, triggered oxidative damage can damage many tissues, including protein and lipid oxidation as well as DNA damage [ 25 ]. In recent years, evidence has been presented that oxidative stress may be more than a simple consequence of inflammation and that some antioxidants that lower oxidative stress markers may provide additional therapeutic support, although not yet conclusively proven, in cases where standard treatment of periodontitis has failed [ 26 ].…”

The translation initiation factor homolog eif4e1c regulates cardiomyocyte metabolism and proliferation during heart regeneration

“…For zebrafish kidney, we obtained datasets from two different studies [two replicates from : and , and three replicates from : , , ( Sun et al, 2022 )]. The zebrafish liver datasets were derived from one study, ( Heinkele et al, 2021 ) consisting of six replicates ( ). The spinal cord datasets were obtained from two studies: (two replicates) ( Saraswathy et al, 2022 ) and (one replicate) ( Mokalled et al, 2016 ).…”

The Translation Initiation Factor Homolog,eif4e1c, Regulates Cardiomyocyte Metabolism and Proliferation During Heart Regeneration

“…For zebrafish kidney, we obtained datasets from 2 different studies (2 replicates from GSE158079: GSM4790350 and GSM4790351, and 3 replicates from GSE193630: GSM5815254, GSM5815255, GSM5815256 (Sun et al, 2022)). The zebrafish liver datasets were derived from 1 study, GSE183023 (Heinkele et al, 2021) consisting of 6 replicates (GSM5549094). The spinal cord datasets were obtained from 2 studies: GSE183644 (2 replicates) (Saraswathy et al, 2022) and GSE77025 (1 replicate) (Mokalled et al, 2016).…”