Metabolic and ultrastructural characteristics of biosynthetic processes in the cerebral cortex at 3 months following a prolonged hemorrhagic shock

Кожура, В. Л.; Solov'eva, Zh V; Novoderzhkina, I. S.; Смирнова, Н. В.

doi:10.1016/0300-9572(91)90081-9

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…Therefore, we attempted to investigate the effects of these peptides as components of mixtures with their doses reduced to subthreshold. Simultaneous intraarterial infusion of FMRFa and TRH in subthreshold doses (0.01 mg/kg/min) improved the recovery of arterial pressure after hemorrhagic shock.It is especially interesting to analyze the effects of such a mixture on the behavioral reactions in the posthemorrhagic period, when persistent changes develop in the metabolic and ultrastructural charac- teristics of biosynthetic processes in the brain [8]. Hence, we analyzed the aftereffect of this peptide mixture on the neurological status.…”

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confidence: 99%

Effect of peptidergic correction of hemorrhagic shock on the behavior of rats in the posthemorrhagic period

Bastrikova¹,

Krushinskaya²,

Дубынин³

et al. 1995

Bull Exp Biol Med

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Hemorrhagic shock is shown to disturb the behavior of rats in the open field test. Infusion of a mixture of thyrotropin releasing hormone and FMRFa (Phe-Met-Arg-Phe amide) in subthreshold doses led to a partial recovery of the neurological status. Key Words: hemorrhagic shock; thyrotropin releasing hormone; FMRFa; naloxone; neurological statusAdministration of opioid antagonists such as naloxone is one way to correct hemorrhagic shock [7], However, some claim that naloxone is ineffective and even exerts negative effects in hemorrhagic shock [2,6]. Hence, we decided to investigate the mode of action of endogenous antiopioids.Previously we showed that some peptides (thyrotropin releasing hormone -TRH and Phe-MetArg-Phe amide -FMRFa) suppressing the effects of opioids can exert a pronounced protective effect on the cardio-and hemodynamics in narcotized and awake rats with hemorrhagic shock, although in many cases it is associated with side effects. Therefore, we attempted to investigate the effects of these peptides as components of mixtures with their doses reduced to subthreshold. Simultaneous intraarterial infusion of FMRFa and TRH in subthreshold doses (0.01 mg/kg/min) improved the recovery of arterial pressure after hemorrhagic shock.It is especially interesting to analyze the effects of such a mixture on the behavioral reactions in the posthemorrhagic period, when persistent changes develop in the metabolic and ultrastructural charac- teristics of biosynthetic processes in the brain [8]. Hence, we analyzed the aftereffect of this peptide mixture on the neurological status. MATERIALS AND METHODSHemorrhagic shock was induced in awake rats (outbred females) by letting 40% of the blood through a catheter implanted in the carotid artery under Nembutal (40 mg/kg) narcosis. Immediately after bloodletting, a 40-rain intraarterial infusion of a mixture of FMRFa and TRH (Sigma) in subthreshold doses (0.01 mg/kg/min) was started. Equivalent volumes of normal saline were infused to control animals.The orienting-exploratory activity and emotional status of the animals were assessed on days 2, 4, and 7 after blood loss in the open field test (a stress-free modification) [5]. Horizontal motor activity (HMA), vertical activity, grooming movements, and the number of radial transpositions into the center of the field were visually recorded for 2 min.For additional control, in order to single out the effects of the operation and hemorrhagic shock on behavior, two groups of animals were examined: intact and sham-hemorrhagic. An operation without bloodletting was performed in sham-hemorrhagic rats.

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confidence: 99%

Effect of peptidergic correction of hemorrhagic shock on the behavior of rats in the posthemorrhagic period

Bastrikova¹,

Krushinskaya²,

Дубынин³

et al. 1995

Bull Exp Biol Med

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“…Severe disturbances of ultrastructure and biochemical parameters in the cerebral cortex neurons occur in the late posthemorrhagic period [4]. They may bring about some impairments of the behavioral reactions in animals.…”

Section: Abstract: Hemorrhagic Shock Arterial Hypertension Anxietymentioning

confidence: 99%

Influence of hemorrhagic shock on learning activity in the late posthemorrhagic period

et al. 1998

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Anxiety and learning activity (acquisition of the conditioned behavior reaction trained with a bonus) were investigated in rats during 4 weeks after hemorrhagic shock. Some phasic behavioral alterations have been found in animals undergone hemorrhagic shock. Key words: hemorrhagic shock, arterial hypertension, anxiety, learningNotable alterations develop in animal behavior in the early posthemorrhagic period, manifested as worse memorization of experimental situation [2].Severe disturbances of ultrastructure and biochemical parameters in the cerebral cortex neurons occur in the late posthemorrhagic period [4]. They may bring about some impairments of the behavioral reactions in animals.We studied the process of learning in rats at different stages of the recovery period after hemorrhagic shock (HS). MATERIALS AND METHODSExperiments were performed on 91 outbred male albino rats (280-340 g). The animals, 6 to 7 in a cage, were housed in standard vivarium conditions with natural day-lighting. Arterial hypotension down to the mean pressure of 40 mmHg was produced in anesthetized rats (Nembutal, 40 mg/kg) by letting of the blood via a catheter inserted into the tail artery. Arterial pressure (AP) was maintained at this level during 1 hour, then the drained blood was reinfused. Learning consisted in acquisition of the food search learning task in T-maze [1] with arm length of 30 cm each. On day 1, deprived of food rats were placed for 1 h into the maze, where balls of bread were scattered, with the aim of adaptation and fatigue of orienting-exploratory activity. During the next 4 days animals were trained. Each day they were placed for 3 min or .less into the maze 5 times, successively. As a bonus were used bread balls. The following parameters were recorded: the latency (period between placing into and leaving starting compartment); the reaction time (the time needed for reaching and taking food); the number of mistakes

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“…Было установлено, что мембраны всех клеточных типов нервной и сосудистой тканей мозга об наруживают ряд существенных и одинаковых по своей природе дефектов (образование ламиллярных структур, отсутствие репаративных процессов). Мембраны всех клеточных типов нервной ткани представляют монослой веществ средней электронной плотностью [8, [20][21][22].…”

Section: вклад профессора в л кожуры в развитие реаниматологииunclassified

Contribution of Professor V. L. Kozhura to the Development of Reanimatology

Новодержкина¹,

Molchanova²,

Poroshenko³

2009

rmt

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Metabolic and ultrastructural characteristics of biosynthetic processes in the cerebral cortex at 3 months following a prolonged hemorrhagic shock

Cited by 4 publications

References 7 publications

Effect of peptidergic correction of hemorrhagic shock on the behavior of rats in the posthemorrhagic period

Effect of peptidergic correction of hemorrhagic shock on the behavior of rats in the posthemorrhagic period

Influence of hemorrhagic shock on learning activity in the late posthemorrhagic period

Contribution of Professor V. L. Kozhura to the Development of Reanimatology

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