Metabolic Aspects of Aerobic Obligate Methanotrophy⋆

Trotsenko, Yu. A.; Murrell, J. Colin

doi:10.1016/s0065-2164(07)00005-6

Cited by 382 publications

(334 citation statements)

References 169 publications

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“…Aerobic methanotrophs are a unique subset of methylotrophic bacteria that have the ability to grow on methane (CH 4 ) as a sole carbon and energy source (Trotsenko and Murrell, 2008;Chistoserdova and Lidstrom, 2013). A defining characteristic of these microorganisms is the use of methane monooxygenase (MMO) enzymes to catalyze the oxidation of methane to methanol.…”

Section: Introductionmentioning

confidence: 99%

“…A defining characteristic of these microorganisms is the use of methane monooxygenase (MMO) enzymes to catalyze the oxidation of methane to methanol. Two forms of MMO have been found, that is, the membrane-associated or particulate MMO and the soluble MMO (Trotsenko and Murrell, 2008;Semrau et al, 2010). Soluble MMO occurs in some species only, while nearly all known methanotrophs possess particulate MMO.…”

Section: Introductionmentioning

confidence: 99%

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A new cell morphotype among methane oxidizers: a spiral-shaped obligately microaerophilic methanotroph from northern low-oxygen environments

et al. 2016

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Although representatives with spiral-shaped cells are described for many functional groups of bacteria, this cell morphotype has never been observed among methanotrophs. Here, we show that spiral-shaped methanotrophic bacteria do exist in nature but elude isolation by conventional approaches due to the preference for growth under micro-oxic conditions. The helical cell shape may enable rapid motility of these bacteria in water-saturated, heterogeneous environments with high microbial biofilm content, therefore offering an advantage of fast cell positioning under desired high methane/low oxygen conditions. The pmoA genes encoding a subunit of particulate methane monooxygenase from these methanotrophs form a new genus-level lineage within the family Methylococcaceae, type Ib methanotrophs. Application of a pmoA-based microarray detected these bacteria in a variety of high-latitude freshwater environments including wetlands and lake sediments. As revealed by the environmental pmoA distribution analysis, type Ib methanotrophs tend to live very near the methane source, where oxygen is scarce. The former perception of type Ib methanotrophs as being typical for thermal habitats appears to be incorrect because only a minor proportion of pmoA sequences from these bacteria originated from environments with elevated temperatures.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A new cell morphotype among methane oxidizers: a spiral-shaped obligately microaerophilic methanotroph from northern low-oxygen environments

et al. 2016

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“…Their contribution would be even higher without the activity of aerobic methane-oxidizing bacteria (MOB), which act as a biofilter, mitigating emissions to the atmosphere (Reeburgh et al, 1993). MOB use methane as the sole source of carbon and energy, provided oxygen is available (Trotsenko and Murrell, 2008). Owing to this dual dependency, they thrive at oxic-anoxic interfaces, where both substrates are supplied (Brune et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

One millimetre makes the difference: high-resolution analysis of methane-oxidizing bacteria and their specific activity at the oxic–anoxic interface in a flooded paddy soil

et al. 2012

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Aerobic methane-oxidizing bacteria (MOB) use a restricted substrate range, yet >30 species-equivalent operational taxonomical units (OTUs) are found in one paddy soil. How these OTUs physically share their microhabitat is unknown. Here we highly resolved the vertical distribution of MOB and their activity. Using microcosms and cryosectioning, we sub-sampled the top 3-mm of a water-saturated soil at near in situ conditions in 100-μm steps. We assessed the community structure and activity using the particulate methane monooxygenase gene pmoA as a functional and phylogenetic marker by terminal restriction fragment length polymorphism (t-RFLP), a pmoA-specific diagnostic microarray, and cloning and sequencing. pmoA genes and transcripts were quantified using competitive reverse transcriptase PCR combined with t-RFLP. Only a subset of the methanotroph community was active. Oxygen microprofiles showed that 89% of total respiration was confined to a 0.67-mm-thick zone immediately above the oxic–anoxic interface, most probably driven by methane oxidation. In this zone, a Methylobacter-affiliated OTU was highly active with up to 18 pmoA transcripts per cell and seemed to be adapted to oxygen and methane concentrations in the micromolar range. Analysis of transcripts with a pmoA-specific microarray found a Methylosarcina-affiliated OTU associated with the surface zone. High oxygen but only nanomolar methane concentrations at the surface suggested an adaptation of this OTU to oligotrophic conditions. No transcripts of type II methanotrophs (Methylosinus, Methylocystis) were found, which indicated that this group was represented by resting stages only. Hence, different OTUs within a single guild shared the same microenvironment and exploited different niches.

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“…These are distributed among Gammaproteobacteria (type I methanotrophs), Alphaproteobacteria (type II methanotrophs) (reviewed in Trotsenko and Murrell, 2008), filamentous methane oxidizers (Stoecker et al, 2006;Vigliotta et al, 2007) and Verrucomicrobia (Dunfield et al, 2007;Pol et al, 2007;Islam et al, 2008). Methanotrophs oxidize methane to methanol by the enzyme methane monooxygenase (MMO), present either as the particulate form (pMMO) in all characterized methanotrophs (except in the genus Methylocella (Dedysh et al, 2000)) or as the soluble form (sMMO) in some methanotrophs (Trotsenko and Murrell, 2008). Methanol dehydrogenase (MDH) catalyzes the conversion of methanol to formaldehyde in methylotrophs Murrell, 2008, Chistoserdova et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact

et al. 2010

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Lonar Lake is a unique saline and alkaline ecosystem formed by meteor impact in the Deccan basalts in India around 52 000 years ago. To investigate the role of methylotrophy in the cycling of carbon in this unusual environment, stable-isotope probing (SIP) was carried out using the onecarbon compounds methane, methanol and methylamine. Denaturing gradient gel electrophoresis fingerprinting analyses performed with heavy 13 C-labelled DNA retrieved from sediment microcosms confirmed the enrichment and labelling of active methylotrophic communities. Clone libraries were constructed using PCR primers targeting 16S rRNA genes and functional genes. Methylomicrobium, Methylophaga and Bacillus spp. were identified as the predominant active methylotrophs in methane, methanol and methylamine SIP microcosms, respectively. Absence of mauA gene amplification in the methylamine SIP heavy fraction also indicated that methylamine metabolism in Lonar Lake sediments may not be mediated by the methylamine dehydrogenase enzyme pathway. Many gene sequences retrieved in this study were not affiliated with extant methanotrophs or methylotrophs. These sequences may represent hitherto uncharacterized novel methylotrophs or heterotrophic organisms that may have been cross-feeding on methylotrophic metabolites or biomass. This study represents an essential first step towards understanding the relevance of methylotrophy in the soda lake sediments of an unusual impact crater structure.

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Metabolic Aspects of Aerobic Obligate Methanotrophy⋆

Cited by 382 publications

References 169 publications

A new cell morphotype among methane oxidizers: a spiral-shaped obligately microaerophilic methanotroph from northern low-oxygen environments

A new cell morphotype among methane oxidizers: a spiral-shaped obligately microaerophilic methanotroph from northern low-oxygen environments

One millimetre makes the difference: high-resolution analysis of methane-oxidizing bacteria and their specific activity at the oxic–anoxic interface in a flooded paddy soil

Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact

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