Metabolic changes during rooting in stem cuttings of five mangrove species

Abstract

Vegetative propagation through rooting in stem cuttings in five tree mangroves namely Bruguiera parviJlora, Cynometra iripa, Excoecaria agallocha, Heritiera fames, and Thespesia populnea using IAA, IBA and NAA was reported. Spectacular increase in the root number was noted in the cuttings of H. fames and C. iripa treated together with IBA (5000 ppm) and NAA (2500 ppm). The highest number of roots w+s obtained with IBA (2500 ppm) and NAA (500 ppm) in E. agallocha. B. parvijora and T populnea responded better to IAA and IBA treatment. The species specific variation in the rooting response to exogenous application of auxins was reflected in the metabolic changes during initiation and development of roots in cuttings. Biochemical analysis showed increase of reducing sugar in the above-girdled tissues at initiation as well as subsequent development of roots which was further enhanced by the use of auxins. Decreases in the total sugar, total carbohydrate and polyphenols and increase in total nitrogen were recorded in the girdled tissues and the high C/N ratio at the initial stage helped in initiation of roots in all the species. Interaction of IBA and NAA promoted starch hydrolysis better than IAA and IBA during root development and subsequently reduced the C/N ratio and increased the protein-nitrogen activity during root development which suggest the auxin influenced mobilization of nitrogen to the rooting zone.Abbreviations: IAA = Indole-3-acetic acid; IBA = Indole-butyric acid; NAA = A-naphthalene acetic acid.

Growth performance of Ceriops decandra (Griff.) Ding Hou propagules as influenced by PGR – a conservation effort

“…In the present study, combined treatment with IBA and NAA also produced highest rooting percentage, root number and length in both the types of cuttings over the controls. The stimulatory effects of auxins on adventitious rooting of stem cuttings and air layers of several other mangrove and non-mangrove species have been reported earlier (Basak et al 1995, 2000, Das et al 1997, Davis and Haissig 1994, Hartmann et al 1997. This study thus establishes good promise of a cost effective and promising technique of propagation for the endangered mangrove species for raising populations of superior clones for planting in seed orchards or directly in the field to aid in our efforts to conserve mangrove species.…”

“…Exogenously applied, gibberellin promoted stolon elongation and inhibited tuber formation and increased fresh weight in potato (Xu et al 1998). Combined auxin treatment was reported to have strong effect on the rooting response of stem cuttings/air layers of difficult-to-root species (Basak et al 1995, Jackson 1986). In the present study, combined treatment with IBA and NAA also produced highest rooting percentage, root number and length in both the types of cuttings over the controls.…”

Physiological Aspects in Propagation of Ninebark (<i>Physocarpus opulifolius </i> Maxim. ‘Red Baron’) by Stem Cuttings Treated with Auxin or Biostimulator

“…In tomato and sweet pepper, biostimulators Asahi SL i Biochicol 020 reduced the levels of polyphenolic acids relative to untreated controls (Cwalina-Ambroziak and Amarowicz, 2012). Basak et al (1995) observed a drop in polyphenolic acids resulting from application of the mixture of IAA and IBA: from 15% in cuttings of Cynometra iripa, Heritiera fomes and Thespesia populnea to 40% in Bruguiera parviflora.…”

Shoot Girdling Improves Rooting Performance of Kalamata Olive Cuttings by Upregulating Carbohydrates, Polyamines and Phenolic Compounds

“…Basak et al [11] found that girdling increased soluble sugar concentration and the rooting ability in mangrove trees. Additionally, girdling was found to be the most effective method for the rooting of cv.…”

“…Girdling of the shoot phloem is a horticultural practice that blocks the downward translocation of carbohydrates, hormones, and other possible root-promoting factors [4,11]. The resulting series of physiological changes, such as the accumulation of various organic substances that occur above the ring, affect the hormonal balance of the shoot, thus promoting root initiation [12,13].…”