Metabolic characterization of a strain (BM90) of Delftia tsuruhatensis showing highly diversified capacity to degrade low molecular weight phenols

Juárez-Jiménez, B.; Manzanera, Maximino; Rodelas, B.; Martı́nez-Toledo, M.V.; González‐López, Jesús; Crognale, Silvia; Pesciaroli, Chiara; Fenice, Massimiliano

doi:10.1007/s10532-009-9317-4

Cited by 41 publications

(26 citation statements)

References 47 publications

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“…Delftia sp. was described as forming part of cellulolytic communities in several ecological niches (Juárez-Jiménez et al, 2010), including soils (Mehnaz et al, 2010). This genus was also isolated from a filter paper culture of native soil (Talia et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Lignocellulosic Diets on Bacterial Microbiota and Hydrolytic Enzyme Activities in the Gut of the Cotton Boll Weevil (Anthonomus grandis)

et al. 2016

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Cotton boll weevils, Anthonomus grandis, are omnivorous coleopteran that can feed on diets with different compositions, including recalcitrant lignocellulosic materials. We characterized the changes in the prokaryotic community structure and the hydrolytic activities of A. grandis larvae fed on different lignocellulosic diets. A. grandis larvae were fed on three different artificial diets: cottonseed meal (CM), Napier grass (NG) and corn stover (CS). Total DNA was extracted from the gut samples for amplification and sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. Proteobacteria and Firmicutes dominated the gut microbiota followed by Actinobacteria, Spirochaetes and a small number of unclassified phyla in CM and NG microbiomes. In the CS feeding group, members of Spirochaetes were the most prevalent, followed by Proteobacteria and Firmicutes. Bray–Curtis distances showed that the samples from the CS community were clearly separated from those samples of the CM and NG diets. Gut extracts from all three diets exhibited endoglucanase, xylanase, β-glucosidase and pectinase activities. These activities were significantly affected by pH and temperature across different diets. We observed that the larvae reared on a CM showed significantly higher activities than larvae reared on NG and CS. We demonstrated that the intestinal bacterial community structure varies depending on diet composition. Diets with more variable and complex compositions, such as CS, showed higher bacterial diversity and richness than the two other diets. In spite of the detected changes in composition and diversity, we identified a core microbiome shared between the three different lignocellulosic diets. These results suggest that feeding with diets of different lignocellulosic composition could be a viable strategy to discover variants of hemicellulose and cellulose breakdown systems.

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Section: Discussionmentioning

confidence: 99%

Effect of Different Lignocellulosic Diets on Bacterial Microbiota and Hydrolytic Enzyme Activities in the Gut of the Cotton Boll Weevil (Anthonomus grandis)

et al. 2016

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“…It is a gramnegative, straight, or slightly curved rod and a facultative methylotroph capable of growing on a variety of C1 and multicarbon compounds 21) . Bands 5-7 and band 8 showed 99% and 97% similarity, respectively, to Delftia tsuruhatensis BM90, which is a gramnegative, motile rod with a broad capacity for utilizing fatty, organic, and amino acids, although its capacity for utilizing carbohydrates is very poor 22) . Band 9 showed 92% similarity to Spirochaetes bacterium SA-10.…”

Section: Identification Of Bacteria By Pcr-dgge Analysismentioning

confidence: 99%

Detection of Dissimilatory Iron-Reducing <i>Deltaproteobacteria</i> in Anaerobic Sludge with High Mineral Content

Sekikawa

Hayashi

Iwahori

2013

Japanese J. Wat. Treat. Biol.

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Several species of dissimilatory iron-reducing Deltaproteobacteria (DIRD) have been isolated and studied worldwide; they have various applications in microbial fuel cells and in bioremediation strategies such as dissimilatory iron reduction. In this study, we detected DIRD in anaerobic sludge with a high mineral content (Na, K, Ca, and Fe) taken from an anaerobic tank at a landfill leachate treatment system (LLTS) and the first anaerobic filter chamber of an advanced Johkasou system that uses iron electrolysis (AJIE). Ferrihydrite media treated with LLTS or AJIE samples turned black and showed magnetic response. A bacterium closely related to Desulfuromonas palmitatis SDBY1 was found in the LLTS anaerobic sludge by PCR-denaturing gradient gel electrophoresis analysis after incubation. However, no single clear band derived from DIRD was found in the AJIE sample after incubation, although it produced magnetite. The number of DIRD in the anaerobic sludge was quantified by quantitative real-time PCR using a DIRD-specific primer set, and subsequently PCR amplicons derived from DIRD were detected in media containing the LLTS and AJIE samples. DNA sequence analysis of a PCR amplicon from the AJIE sample identified a bacterium closely related to Geobacter lovleyi SZ.

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“…In this context, the search of new high EPS producers is still important to find new applications or better fit traditional uses. Moreover, strain physiologic and metabolic characterization is extremely useful to understand and optimize microbial productions [30,31]. …”

Section: Introductionmentioning

confidence: 99%

Physiologic and metabolic characterization of a new marine isolate (BM39) of Pantoea sp. producing high levels of exopolysaccharide

et al. 2013

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BackgroundMarine environments are the widest fonts of biodiversity representing a resource of both unexploited or unknown microorganisms and new substances having potential applications. Among microbial products, exopolysaccharides (EPS) have many physiological functions and practical applications. Since EPS production by many bacteria is too scarce for practical use and only few species are known for their high levels of production, the search of new high EPS producers is of paramount importance. Many marine bacteria, that produce EPS to cope with strong environmental stress, could be potentially exploited at the industrial level.ResultsA novel bacterium, strain BM39, previously isolated from sediments collected in the Tyrrhenian Sea, was selected for its production of very high levels of EPS. BM39 was affiliated to Pantoea sp. (Enterobacteriaceae) by 16S rRNA gene sequencing and biochemical tests. According to the phylogenetic tree, this strain, being quite far from the closest known Pantoea species (96% identity with P. agglomerans and P. ananatis) could belong to a new species. EPS production was fast (maximum of ca. 21 g/L in 24 h on glucose medium) and mainly obtained during the exponential growth. Preliminary characterization, carried out by thin layer and gel filtration chromatography, showed that the EPS, being a glucose homopolymer with MW of ca. 830 kDa, appeared to be different from those of other bacteria of same genus. The bacterium showed a typical slightly halophilic behavior growing optimally at NaCl 40 ‰ (growing range 0-100 ‰). Flow cytometry studies indicated that good cell survival was maintained for 24 h at 120 ‰. Survival decreased dramatically with the increase of salinity being only 1 h at 280 ‰. The biochemical characterization, carried out with the Biolog system, showed that MB39 had a rather limited metabolic capacity. Its ability, rather lower than that of P. agglomerans, was almost only confined to the metabolization of simple sugars and their derivatives. Few alcohols, organic acids and nitrogen compounds were partially used too.ConclusionsStrain BM39, probably belonging to a new species, due to its remarkable EPS production, comparable to those of known industrial bacterial producers, could be suggested as a new microorganism for industrial applications.

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Metabolic characterization of a strain (BM90) of Delftia tsuruhatensis showing highly diversified capacity to degrade low molecular weight phenols

Cited by 41 publications

References 47 publications

Effect of Different Lignocellulosic Diets on Bacterial Microbiota and Hydrolytic Enzyme Activities in the Gut of the Cotton Boll Weevil (Anthonomus grandis)

Effect of Different Lignocellulosic Diets on Bacterial Microbiota and Hydrolytic Enzyme Activities in the Gut of the Cotton Boll Weevil (Anthonomus grandis)

Detection of Dissimilatory Iron-Reducing <i>Deltaproteobacteria</i> in Anaerobic Sludge with High Mineral Content

Physiologic and metabolic characterization of a new marine isolate (BM39) of Pantoea sp. producing high levels of exopolysaccharide

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