Metabolic characterization of natural and cultured Ophicordyceps sinensis from different origins by 1H NMR spectroscopy

Zhang, Jianshuang; Zhong, Xin; Li, Shaosong; Zhang, Guren; Liu, Xin

doi:10.1016/j.jpba.2015.07.035

Cited by 31 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NMR spectroscopy plays important and multifaceted roles that have benefited and continue to benefit the field of metabolomics. These include biomarker discovery [51,259,260,261,262], kinetic and mechanistic metabolic studies [263,264,265,266,267], metabolite imaging [268,269,270], food and beverage characterization [271,272,273,274], environmental monitoring [275,276,277], and drug toxicity studies [278,279,280,281,282,283,284]. The unique characteristics of NMR, including its high level of reproducibility, its simplicity in sample preparation, its capacity to handle diverse sample types (liquids, solids, gels), its quantitative capabilities, and its utility in identifying unknown metabolites along with its nondestructive nature, have made it particularly useful in many diverse metabolomic disciplines.…”

Section: Concluding Remarks and Future Prospectsmentioning

confidence: 99%

NMR Spectroscopy for Metabolomics Research

et al. 2019

View full text Add to dashboard Cite

Over the past two decades, nuclear magnetic resonance (NMR) has emerged as one of the three principal analytical techniques used in metabolomics (the other two being gas chromatography coupled to mass spectrometry (GC-MS) and liquid chromatography coupled with single-stage mass spectrometry (LC-MS)). The relative ease of sample preparation, the ability to quantify metabolite levels, the high level of experimental reproducibility, and the inherently nondestructive nature of NMR spectroscopy have made it the preferred platform for long-term or large-scale clinical metabolomic studies. These advantages, however, are often outweighed by the fact that most other analytical techniques, including both LC-MS and GC-MS, are inherently more sensitive than NMR, with lower limits of detection typically being 10 to 100 times better. This review is intended to introduce readers to the field of NMR-based metabolomics and to highlight both the advantages and disadvantages of NMR spectroscopy for metabolomic studies. It will also explore some of the unique strengths of NMR-based metabolomics, particularly with regard to isotope selection/detection, mixture deconvolution via 2D spectroscopy, automation, and the ability to noninvasively analyze native tissue specimens. Finally, this review will highlight a number of emerging NMR techniques and technologies that are being used to strengthen its utility and overcome its inherent limitations in metabolomic applications.

show abstract

Section: Concluding Remarks and Future Prospectsmentioning

confidence: 99%

NMR Spectroscopy for Metabolomics Research

et al. 2019

View full text Add to dashboard Cite

show abstract

“…However, one study also reported that the contents of crude fat, total amino acids, and minerals were significantly different between natural and cultured samples [20]. Natural and cultured samples display significant differences in their metabolic profiles [21]. Thus, the available findings are confusing.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years

et al. 2019

View full text Add to dashboard Cite

Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years. Therefore, it is important for cultivated C. sinensis to be recognized in the market. In this research, the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years were detected and compared by HPLC-MS and UV spectrometry. The results indicated that the mean levels of adenosine and cordycepin were significantly higher, whereas the mean levels of mannitol and polysaccharides were remarkably lower in the cultivated type than in the natural type. No distinct difference in the average soluble protein content was observed. The composition of the stiff worms was similar to that of the natural herb, except that the total soluble protein content was higher, and that of mannitol was lower. In addition, the ultraviolet absorption spectroscopy of the three types showed high similarity at 260 nm. This research indicates that the main nutritional composition of cultivated and natural C. sinensis is identical and that cultivated type can be used as an effective substitute.

show abstract

“…As reported by Jing et al [12], the quality of polysaccharides in the fruiting bodies of Flammulina velutipes from three provinces in China were assessed and significant differences could be found in the tested samples. Similarly, some researchers also reported that the Ophicordyceps sinensis obtained from different geographical origins could be discriminated by the levels of mannitol, trehalose and some amino acids [13]. Therefore, the places from which the edible mushrooms collected are crucial if the specimens are to be of good qualities.…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive and Comparative Study of Wolfiporia extensa Cultivation Regions by Fourier Transform Infrared Spectroscopy and Ultra-Fast Liquid Chromatography

Zhang

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

Nowadays, Wolfiporia extensa as a popular raw material in food and medicine industry has received increasing interests. Due to supply shortage, this species of edible and medicinal mushroom has been cultivated in some provinces of China. In the present study, cultivated W. extensa collected from six regions in Yunnan Province of China were analyzed by an integrated method based on Fourier transform infrared (FT-IR) spectroscopy and ultra-fast liquid chromatography (UFLC) coupled with multivariate analysis including partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) in order to investigate the differences and similarities in different origins and parts. In the tested mushroom samples, characteristic FT-IR spectra were obtained for acquiring comprehensive fuzz chemical information and pachymic acid was determinated as a biomarker in the meantime. From the results, the comparison of samples was achieved successfully according to their geographical regions and different parts. All the samples displayed regional dependence and the inner parts showed better quality consistency. In addition, the chemical constituents of cultivated W. extensa could be also affected by the cultivation methods. Meanwhile, there was an interesting finding that the soil properties of cultivation regions may have a relationship with the chemical constituents of the epidermis of soil-cultured W. extensa, rather than the inner parts. Collectively, it demonstrated that the present study could provide comprehensive chemical evidence for the critical complement of quality evaluation on the cultivated W. extensa. Moreover, it may be available for the further researches of complicated mushrooms in practice.

show abstract

Metabolic characterization of natural and cultured Ophicordyceps sinensis from different origins by 1H NMR spectroscopy

Cited by 31 publications

References 27 publications

NMR Spectroscopy for Metabolomics Research

NMR Spectroscopy for Metabolomics Research

Comparative study of the composition of cultivated, naturally grown Cordyceps sinensis, and stiff worms across different sampling years

A Comprehensive and Comparative Study of Wolfiporia extensa Cultivation Regions by Fourier Transform Infrared Spectroscopy and Ultra-Fast Liquid Chromatography

Contact Info

Product

Resources

About