Metabolic Clearance Rate of Growth Hormone in Mice during Various Physiological States*

Sinha, Y. N.; Baxter, S. R.; Vanderlaan, W. P.

doi:10.1210/endo-105-3-685

Cited by 13 publications

(3 citation statements)

References 17 publications

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“…Neonatal pigs in this study exhibited ipPRL clearance rates comparable to those of humans (Molitch et al, 19871, sheep (Akbar et al, 19741, cattle (Akers et al, 19801, and rats (Koch et al, 1971). For ipGH, the apparent MCRkilogram correspond well with data reported by Althen and Gerrits (1976) for older pigs, as well as with values reported in other species (Thompson et al, 1972;Sinha et al, 1979;Power et al, 1988). Some investigators have chosen to express MCRiunit of metabolic BW (kg.75), which permits the expression of MCR of an animal independent of its body size (Davis and Borger, 1973).…”

Section: Discussionsupporting

confidence: 78%

“…The pharmacokinetic characteristics of ipPRL and ipGH were assessed using the single injection method, which should provide the same results as the constant infusion method (Tait, 1963) and which has been used to assess MCR of PRL and GH in pigs (Spencer et al, 1986;Arbona et al, 1988) and in other species (Sinha et al, 1979;Power et al, 1988). This approach was used because of the young age of the suckling pigs.…”

Section: Discussionmentioning

confidence: 99%

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Prolactin and growth hormone clearance in neonatal boars

Brown‐Borg

Klemcke²,

Borg³

et al. 1993

Journal of Animal Science

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The pharmacokinetics of prolactin (PRL) and growth hormone (GH) are not known in neonatal pigs. In this study, six boars, ages 13 and 26 d, were used to determine metabolic clearance rate (MCR), half-life (t1/2), and volume of distribution (Vd) of immunoreactive porcine PRL (ipPRL) and immunoreactive porcine GH (ipGH). Blood samples were collected through indwelling jugular catheters at 0, 1, 2.5, 5, 10, 15, 30, 45, and 60 min after a 20 ng/g of BW (per hormone) cocktail of porcine PRL and GH. Analysis of the ipPRL disappearance curves indicated an apparent MCR of 13.79 +/- 2.45 and 23.79 +/- 4.10 mL/min for boars at 13 and 26 d of age, respectively (P = .0008). The t1/2 of ipPRL did not differ between ages (P = .9201). The apparent MCR of ipGH at 13 and 26 d were 10.60 +/- 2.09 and 27.30 +/- 4.55 mL/min, respectively, and this difference was still evident after adjustment for BW (P = .0465). The ipGH t1/2 decreased with age (P = .0232), 21.32 +/- 2.55 vs 13.57 +/- 3.35 min for 13 and 26 d, respectively. The apparent distribution volume for ipPRL was higher than that for ipGH at both ages (P = .0021). Within each age, the apparent MCR of ipGH and ipPRL did not differ significantly. These data represent the first clearance measures reported for ipPRL and ipGH in neonatal pigs and indicate that under the conditions of this study the apparent MCR of ipPRL and ipGH are similar but change significantly during early development.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Prolactin and growth hormone clearance in neonatal boars

Brown‐Borg

Klemcke²,

Borg³

et al. 1993

Journal of Animal Science

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“…suggested that the mechanism for this might involve a rise in blood GH concentrations via the appearance of a specific GH binding protein, proposed by Peeters & Friesen (1976) (but see Sinha, Baxter & VanderLaan, 1979). There are no reports of blood or pituitary levels of GH in pregnant Little mice so an explanation of this growth awaits further study.…”

Section: Discussionmentioning

confidence: 99%

Effects of a fragment of human growth hormone-releasing factor in normal and 'Little' mice

Clark¹,

Robinson²

1985

Journal of Endocrinology

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The 'Little' mouse is characterized by a body growth rate 60% of normal due to a defect in the synthesis and storage of GH in the anterior pituitary gland. We have now investigated the effects of GH releasing factor (GRF) in these mice and in normal animals. The pituitary GH content in Little mice was only 4% of that in normal C57: +/+ mice, and was not affected by twice daily i.p. injections of human (h) GRF1-29NH2 (0.2-2 micrograms) for 14 days. This treatment also had no effect on body growth. In anaesthetized normal mice, single i.v. injections of 0.1 or 2 micrograms hGRF1-29NH2 released large amounts of GH into the plasma, whereas this peptide was ineffective in Little mice, whether or not they had been pretreated with GRF. Therefore, although pituitaries of Little mice contain significant amounts of GH, this pool is not releasable by GRF. This suggests that the dwarfism in Little mice may be partly due to a pituitary defect in GRF receptors or their stimulus-secretion coupling, rather than a deficiency in hypothalamic GRF.

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