Metabolic DNA in Heart and Skeletal Muscle and in the Intestine of Mice

Stroun, Maurice; Charles, P; Anker, Philippe; Pelc, S. R.

doi:10.1038/216716a0

Cited by 44 publications

(16 citation statements)

References 5 publications

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“…The specific activity of the low molecular weight fractions was significantly higher than that of the high molecular weight fractions. The evidence for low molecular weight DNA in mammalian tissues was in accordance with Sampson's low molecular weight DNA identified in plant tissues [149].…”

Section: Macronuclei and Metabolic Dnasupporting

confidence: 63%

“…A low molecular weight fraction (between 5 9 10 5 and 4 9 10 6 ) and high molecular weight fraction (above 4 9 10 6 ) were obtained [149]. The specific activity of the low molecular weight fractions was significantly higher than that of the high molecular weight fractions in the three organs.…”

Section: Pelc's Metabolic Dnamentioning

confidence: 87%

“…The specific activity of the low molecular weight fractions was significantly higher than that of the high molecular weight fractions in the three organs. Stroun et al [149] theorized that the high specific activity of the low molecular weight fraction, especially in non-dividing muscle tissue, was possibly due to this DNA existing as a separate fraction in mammalian cells, and connected this DNA to the DNA responsible for the metabolic activity observed in the previous studies by Pelc, and referred to it as metabolic DNA. Pelc summarized the characteristics of metabolic DNA in 1968: It has been determined that (a) stable and metabolic DNA existed in cells of higher organisms; (b) metabolic DNA is formed immediately after the last mitosis in some organs and may be synthesized during or immediately after premitotic synthesis but before mitosis; (c) metabolic DNA is renewed or repaired periodically instead of continuously; (d) metabolic DNA is closely connected with the functional activity of differentiated cells; and (e) changes in DNA content, incorporation of precursors, or loss of labelled DNA correlates with a definite stage in development or with the stimulation of activity, indicating a definite correlation of metabolic DNA with function [150,151].…”

Section: Pelc's Metabolic Dnamentioning

confidence: 99%

“…Connection between low molecular weight DNA and Pelc's metabolically active DNA -METABOLIC DNA [149].…”

Section: Macronuclei and Metabolic Dnamentioning

confidence: 99%

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A historical and evolutionary perspective on the biological significance of circulating DNA and extracellular vesicles

Aucamp

Bronkhorst

Badenhorst

et al. 2016

Cell. Mol. Life Sci.

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The discovery of quantitative and qualitative differences of the circulating DNA (cirDNA) between healthy and diseased individuals inclined researchers to investigate these molecules as potential biomarkers for non-invasive diagnosis and prognosis of various pathologies. However, except for some prenatal tests, cirDNA analyses have not been readily translated to clinical practice due to a lack of knowledge regarding its composition, function, and biological and evolutionary origins. We believe that, to fully grasp the nature of cirDNA and the extracellular vesicles (EVs) and protein complexes with which it is associated, it is necessary to probe the early and badly neglected work that contributed to the discovery and development of these concepts. Accordingly, this review consists of a schematic summary of the major events that developed and integrated the concepts of heredity, genetic information, cirDNA, EVs, and protein complexes. Cir-DNA enters target cells and provokes a myriad of gene regulatory effects associated with the messaging functions of various natures, disease progression, somatic genome variation, and transgenerational inheritance. This challenges the traditional views on each of the former topics. All of these discoveries can be traced directly back to the iconic works of Darwin, Lamarck, and their followers. The history of cirDNA that has been revisited here is rich in information that should be considered in clinical practice, when designing new experiments, and should be very useful for generating an empirically up-to-date view of cirDNA and EVs. Furthermore, we hope that it will invite many flights of speculation and stimulate further inquiry into its biological and evolutionary origins.

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Section: Macronuclei and Metabolic Dnasupporting

confidence: 63%

Section: Pelc's Metabolic Dnamentioning

confidence: 87%

Section: Pelc's Metabolic Dnamentioning

confidence: 99%

“…Connection between low molecular weight DNA and Pelc's metabolically active DNA -METABOLIC DNA [149].…”

Section: Macronuclei and Metabolic Dnamentioning

confidence: 99%

See 2 more Smart Citations

A historical and evolutionary perspective on the biological significance of circulating DNA and extracellular vesicles

Aucamp

Bronkhorst

Badenhorst

et al. 2016

Cell. Mol. Life Sci.

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show abstract

“…The significance of the weakly labeled nuclei found in many organs has been evaluated and discussed by Pelc (9), who suggests that this type of incorporation is not due to pre-mitotic DNA synthesis, but rather to a metabolic turnover of DNA (9) . The existence of a DNA fraction amounting to approximately 40% of the total DNA, which is characterized by a high turnover, has recently been demonstrated in the intestine, heart, and skeletal muscle of adult mice (21) . It has been suggested (22,23) that "metabolic" DNA consists of additional copies of those genes which are active in the differentiated cells of an organ and that these copies perform the actual function of DNA, e .g ., transcription to mRNA .…”

Section: Discussionmentioning

confidence: 99%

Iii. Decrease of Labeled Dna in Cells of the Adrenal Medulla After Intermittent Exposure to Cold

Pelc

Viola-Magni

1969

The Journal of Cell Biology

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Italico rats were injected with thymidine 3H 6 hr after the end of 300 hr of intermittent cold treatment . This plan of experiment ensured replacement in the adrenal medulla of lost DNA which is specifically sensitive to cold treatment and has a labeling index sufficiently high for statistical evaluation . The labeling index in the adrenal medulla decreases to one-half of the initial value within 10 days in animals subjected to further intermittent cold treatment and within 32 days in animals kept at room temperature . The very low mitotic index and the absence of doubling of the labeling index show that the observed labeling cannot be ascribed to pre-mitotic DNA synthesis . The concept of metabolic DNA adequately explains the findings .

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Early effects of ultraviolet light on DNA synthesis in human skin in vivo

Epstein¹

1969

Archives of Dermatology

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Metabolic DNA in Heart and Skeletal Muscle and in the Intestine of Mice

Cited by 44 publications

References 5 publications

A historical and evolutionary perspective on the biological significance of circulating DNA and extracellular vesicles

A historical and evolutionary perspective on the biological significance of circulating DNA and extracellular vesicles

Iii. Decrease of Labeled Dna in Cells of the Adrenal Medulla After Intermittent Exposure to Cold

Early effects of ultraviolet light on DNA synthesis in human skin in vivo

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