Metabolic efficiency in yeast Saccharomyces cerevisiae in relation to temperature dependent growth and biomass yield

Zakhartsev, Maksim; Yang, Xuelian; Reuß, Matthias; Pörtner, Hans‐Otto

doi:10.1016/j.jtherbio.2015.05.008

Cited by 42 publications

(29 citation statements)

References 51 publications

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“…Deleterious effects of high temperature have been associated to protein inactivation due to denaturation, but there is no evidence that such occurs below 45°C (Caspeta & Nielsen, 2015;Zakhartsev et al, 2015). Whereas low energy for maintenance (ATPm) and high glucose consumption direct metabolism at 30°C, very-high ATPm and "mild" glucose uptake rate lead metabolism at 40°C (Roels, 2009;Zakhartsev et al, 2015). Whereas low energy for maintenance (ATPm) and high glucose consumption direct metabolism at 30°C, very-high ATPm and "mild" glucose uptake rate lead metabolism at 40°C (Roels, 2009;Zakhartsev et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Optimal and maximum growth temperatures for yeast species range from 30°C to 34°C and 40°C to 45°C, respectively (Salvadó et al, 2011). The specific rates of growth and ethanol production decreased rapidly as temperature raised to the maximum (Caspeta & Nielsen, 2015;Zakhartsev, Yang, Reuss, & Pörtner, 2015). Deleterious effects of high temperature have been associated to protein inactivation due to denaturation, but there is no evidence that such occurs below 45°C (Caspeta & Nielsen, 2015;Zakhartsev et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Facts rather suggested that S. cerevisiae suppression is because of imbalances in cellular energy. Whereas low energy for maintenance (ATPm) and high glucose consumption direct metabolism at 30°C, very-high ATPm and "mild" glucose uptake rate lead metabolism at 40°C (Roels, 2009;Zakhartsev et al, 2015). ATPm is mainly required for keeping H + gradients and electrical potentials in the yeast plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

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Engineering high‐gravity fermentations for ethanol production at elevated temperature with Saccharomyces cerevisiae

Caspeta

Coronel

et al. 2019

Biotech & Bioengineering

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Thermal damage, high osmolarity, and ethanol toxicity in the yeast Saccharomyces cerevisiae limit titer and productivity in fermentation to produce ethanol. We show that long-term adaptive laboratory evolution at 39.5°C generates thermotolerant yeast strains, which increased ethanol yield and productivity by 10% and 70%, in 2% glucose fermentations. From these strains, which also tolerate elevated-osmolarity, we selected a stable one, namely a strain lacking chromosomal duplications. This strain (TTY23) showed reduced mitochondrial metabolism and high proton efflux, and therefore lower ethanol tolerance. This maladaptation was bolstered by reestablishing proton homeostasis through increasing fermentation pH from 5 to 6 and/or adding potassium to the media. This change allowed the TTY23 strain to produce 1.3-1.6 times more ethanol than the parental strain in fermentations at 40°C with glucose concentrations~300 g/L. Furthermore, ethanol titers and productivities up to 93.1 and 3.87 g·L −1 ·hr −1 were obtained from fermentations with 200 g/L glucose in potassium-containing media at 40°C. Albeit the complexity of cellular responses to heat, ethanol, and high osmolarity, in this study we overcome such limitations by an inverse metabolic engineering approach.ethanol tolerance, high-temperature fermentation, S. cerevisiae, thermotolerance Abbreviations: AcetylCoA, acetyl coenzyme-A; ADH, alcohol dehydrogenase; ADH4, alcohol dehydrogenase (alcohol dehydrogenase isoenzyme type IV); ADP, adenosine-diphosphate; ALD2, aldehyde dehydrogenase (cytoplasmic aldehyde dehydrogenase); ATP, adenosine-triphosphate; ATP_F0, ATP synthase, F1 complex (membrane-embedded portion; protons are transferred through this); ATP_F1, ATP synthase, F1 complex (hydrophilic catalytic portion which is sticking into matrix); ATP-Stalk, ATP synthase stalk (it is considered as part of F1); bc1, cytochrome B (mitochondrial cytochrome bc1 complex); CytC, cytochrome C oxidase; Glucose-P, glucose-6-phosphate; Glyceraldehyde-P, glyceraldehyde-3-phosphate; GND1, 6-phosphogluconate dehydrogenase; HSP30, heat shock protein 30 (negative regulator of the H + -ATPase PMA1); IDP1, isocitrate dehydrogenase, NADP-specific (mitochondrial NADP-specific isocitrate dehydrogenase); KHA1, K/H ion antiporter (putative K + /H + antiporter); MDH1, malate dehydrogenase (mitochondrial malate dehydrogenase); MPC1, mitochondrial pyruvate carrier (mitochondrial inner membrane complex that mediates pyruvate uptake); MPC3, mitochondrial pyruvate carrier (highly conserved subunit of the mitochondrial pyruvate carrier); NADPH, nicotinamide adenine dinucleotide reduced; NDE1, NADH dehydrogenase, external (mitochondrial external NADH dehydrogenase; type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH); NDE2, NADH dehydrogenase external (mitochondrial external NADH dehydrogenase; catalyzes the oxidation of cytosolic NADH); NDI1, NADH dehydrogenase internal (NADH: ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in respiratory chain ...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Engineering high‐gravity fermentations for ethanol production at elevated temperature with Saccharomyces cerevisiae

Caspeta

Coronel

et al. 2019

Biotech & Bioengineering

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“…Environmental condition of the Saccharomyces cerevisiae was at an unsteady state. The fermentation process took place in line with the constant specific growth rate and did not depend on the change in nutrient concentration [12,13]. Table 1 shows the specific growth rate of Saccharomyces cerevisiae in the medium concentration of 75%.…”

Section: Resultsmentioning

confidence: 99%

Kinetic Growth of Saccharomyces cerevisiae in Non Dairy Creamer Wastewater Medium

2016

J Environ Stud

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Efforts in non dairy creamer (NDC) wastewater treatment can be performed by converting it into raw materials, i.e. as a medium for microorganisms growth. This research had an objective of examining the growth kinetics of Saccharomyces cerevisiae in the fermentation of the non dairy creamer wastewater for protein production. Kinetic study of the growth and fermentation is necessary as an introduction to understanding each process of the fermentation. The research applied an Experimental Design with a full factorial design. Treatments during the experiment consisted of P1 (NDC medium concentration of 25%), P2 (NDC medium concentration of 50%), P3 (NDC medium concentration of 75%), and P4 (NDC medium concentration of 100%). The cellular concentrate intakes were 105, 106, 107, and 108 cell/ml. The experiment resulted in specific growth rate (µ) of 0.240868 (hour-1), maximum specific growth rate (µmax) of 55.7479 (hour-1), with the highest protein contents of 22.15 mg/l at the concentration medium of 75%. IntroductionNon dairy creamer wastewater that migrates from waste disposal has been proven to cause pollution due to inadequate treatment. In addition to reducing water body quality, the wastewater also has bad smell, which also impacts the affected environment. The smell comes from organic compounds within the wastewater. Therefore, proper treatment is important to convert the wastewater into valuable raw materials.There have been many studies concerning wastewater as a more affordable growth medium for microorganisms in order to produce single cell proteins. However, none of the studies has done so with non dairy creamer wastewater. The use of Saccharomyces cerevisiae in the treatment of non dairy creamer wastewater is expected to have a promising prospect in producing single cell proteins. Single cell proteins are dry cells, which can be found in khamir, bacteria, and algae. These biomass microorganisms are potential sources of proteins for human food and livestock feed. The single cell proteins can be an alternative to fulfill the future needs for proteins because they contain particular proteins as well as carbohydrates, fat, mineral, and other nutrients for both human and animals, cited from [1][2][3]. The use of Saccharomyces cerevisiae in the non dairy creamer wastewater treatment is expected to be effective in the production of the single cell proteins.According to laboratory analysis, the non dairy creamer wastewater contained organic matters, in particular carbon in the form of carbohydrate, protein, and fat, so that they have a great potential to be a growth medium for microorganisms. One of the treatments of the non dairy creamer industry wastewater can be converting the wastewater into raw materials. These raw materials are useful for the medium of the microorganism fermentation developed in the production of the single cell proteins.The fermentation process can be assessed through fermentation kinetics. Kinetics study of the growth and fermentation is important as an introduction to understand...

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“…1b). Several studies have revealed that the metabolic changes involved a decreased biomass and a prolonged cell cycle 31, 32 . In addition, research in Daphnia and in rats suggested another flame retardant, Firemaster 550, might also cause metabolic interference 33, 34 .…”

Section: Discussionmentioning

confidence: 99%

The Toxic Effects of Tetrachlorobisphenol A in Saccharomyces cerevisiae Cells via Metabolic Interference

Tian

Wang

et al. 2017

Sci Rep

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Tetrachlorobisphenol A (TCBPA) is a common flame retardant detected in different environments. However, its toxic effects on animals and humans are not fully understood. Here, the differential intracellular metabolites and associated gene expression were used to clarify the metabolic interference of TCBPA in Saccharomyces cerevisiae, a simple eukaryotic model organism. The results indicated that TCBPA treatment promoted the glycolysis pathway but inhibited the tricarboxylic acid (TCA) cycle, energy metabolism and the hexose monophosphate pathway (HMP) pathway. Thus, the HMP pathway produced less reducing power, leading to the accumulation of reactive oxygen species (ROS) and aggravation of oxidative damage. Accordingly, the carbon flux was channelled into the accumulation of fatty acids, amino acids and glycerol instead of biomass production and energy metabolism. The accumulation of these metabolites might serve a protective function against TCBPA stress by maintaining the cell membrane integrity or providing a stable intracellular environment in S. cerevisiae. These results enhance our knowledge of the toxic effects of TCBPA on S. cerevisiae via metabolic interference and pave the way for clarification of the mechanisms underlying TCBPA toxicity in animals and humans.

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Metabolic efficiency in yeast Saccharomyces cerevisiae in relation to temperature dependent growth and biomass yield

Cited by 42 publications

References 51 publications

Engineering high‐gravity fermentations for ethanol production at elevated temperature with Saccharomyces cerevisiae

Engineering high‐gravity fermentations for ethanol production at elevated temperature with Saccharomyces cerevisiae

Kinetic Growth of Saccharomyces cerevisiae in Non Dairy Creamer Wastewater Medium

The Toxic Effects of Tetrachlorobisphenol A in Saccharomyces cerevisiae Cells via Metabolic Interference

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